BRENDA - Enzyme Database show
show all sequences of 2.1.4.2

Enzymic studies on the biosynthesis of streptomycin

Walker, M.S.; Walker, J.B.; J. Biol. Chem. 241, 1262-1270 (1966)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-arginine + 1-amino-1-deoxy-scyllo-inositol 4-phosphate
Streptomyces griseus
-
L-ornithine + 1-guanidino-1-deoxy-scyllo-inositol 4-phosphate
-
-
?
L-arginine + 1-amino-1-deoxy-scyllo-inositol 4-phosphate
Streptomyces griseocarneus
-
L-ornithine + 1-guanidino-1-deoxy-scyllo-inositol 4-phosphate
-
-
?
L-arginine + 1-amino-1-deoxy-scyllo-inositol 4-phosphate
Streptomyces bluensis
-
L-ornithine + 1-guanidino-1-deoxy-scyllo-inositol 4-phosphate
-
-
?
L-arginine + 1-amino-1-deoxy-scyllo-inositol 4-phosphate
Streptomyces kanamyceticus
-
L-ornithine + 1-guanidino-1-deoxy-scyllo-inositol 4-phosphate
-
-
?
L-arginine + 1-amino-1-deoxy-scyllo-inositol 4-phosphate
Streptomyces bikiniensis
biosynthesis of streptomycin
L-ornithine + 1-guanidino-1-deoxy-scyllo-inositol 4-phosphate
-
-
?
L-arginine + 1-amino-1-deoxy-scyllo-inositol 4-phosphate
Streptomyces griseus W4
-
L-ornithine + 1-guanidino-1-deoxy-scyllo-inositol 4-phosphate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
no activity in Streptomyces griseus
-
-
-
no activity in Streptomyces griseus ATCC 10971
-
-
-
Streptomyces bikiniensis
-
ATCC 11062
-
Streptomyces bluensis
-
var. bluensis NRRL 2876
-
Streptomyces griseocarneus
-
ATCC 12628
-
Streptomyces griseus
-
; ATCC 12475
-
Streptomyces griseus W4
-
-
-
Streptomyces kanamyceticus
-
ATCC 12853
-
Purification (Commentary)
Commentary
Organism
-
Streptomyces bluensis
-
Streptomyces bikiniensis
Source Tissue
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Streptomyces kanamyceticus
-
mycelium
-
Streptomyces griseocarneus
-
mycelium
-
Streptomyces bikiniensis
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
arginine + 2-deoxystreptamine 6-phosphate
-
485981
Streptomyces bikiniensis
N-amidino-2-deoxystreptamine 6-phosphate + ornithine
-
-
-
?
arginine + 2-deoxystreptamine 6-phosphate
-
485981
Streptomyces bluensis
N-amidino-2-deoxystreptamine 6-phosphate + ornithine
-
-
-
?
arginine + scyllo-inosamine 4-phosphate
-
485981
Streptomyces bikiniensis
ornithine + N-amidino-scyllo-inosamine-4-phosphate
-
-
-
?
arginine + scyllo-inosamine 4-phosphate
reversibility of transamidinations with the monoamidinated inosamine phosphate has not yet been demonstrated
485981
Streptomyces bluensis
ornithine + N-amidino-scyllo-inosamine-4-phosphate
-
-
-
?
arginine + streptamine 6-phosphate
-
485981
Streptomyces bluensis
ornithine + N-amidinostreptamine 6-phosphate
-
-
-
?
L-arginine + 1-amino-1-deoxy-scyllo-inositol 4-phosphate
-
485981
Streptomyces griseus
L-ornithine + 1-guanidino-1-deoxy-scyllo-inositol 4-phosphate
-
-
-
?
L-arginine + 1-amino-1-deoxy-scyllo-inositol 4-phosphate
-
485981
Streptomyces griseocarneus
L-ornithine + 1-guanidino-1-deoxy-scyllo-inositol 4-phosphate
-
-
-
?
L-arginine + 1-amino-1-deoxy-scyllo-inositol 4-phosphate
-
485981
Streptomyces bluensis
L-ornithine + 1-guanidino-1-deoxy-scyllo-inositol 4-phosphate
-
-
-
?
L-arginine + 1-amino-1-deoxy-scyllo-inositol 4-phosphate
-
485981
Streptomyces kanamyceticus
L-ornithine + 1-guanidino-1-deoxy-scyllo-inositol 4-phosphate
-
-
-
?
L-arginine + 1-amino-1-deoxy-scyllo-inositol 4-phosphate
biosynthesis of streptomycin
485981
Streptomyces bikiniensis
L-ornithine + 1-guanidino-1-deoxy-scyllo-inositol 4-phosphate
-
-
-
?
L-arginine + 1-amino-1-deoxy-scyllo-inositol 4-phosphate
-
485981
Streptomyces griseus W4
L-ornithine + 1-guanidino-1-deoxy-scyllo-inositol 4-phosphate
-
-
-
?
L-arginine + 1-amino-1-deoxy-scyllo-inositol-4-phosphate
-
485981
Streptomyces griseus
L-ornithine + 1-guanidino-1-deoxy-scyllo-inositol 4-phosphate
-
-
-
?
L-arginine + 1-amino-1-deoxy-scyllo-inositol-4-phosphate
-
485981
Streptomyces bikiniensis
L-ornithine + 1-guanidino-1-deoxy-scyllo-inositol 4-phosphate
-
-
-
?
L-arginine + 1-amino-1-deoxy-scyllo-inositol-4-phosphate
-
485981
Streptomyces griseocarneus
L-ornithine + 1-guanidino-1-deoxy-scyllo-inositol 4-phosphate
-
-
-
?
L-arginine + 1-amino-1-deoxy-scyllo-inositol-4-phosphate
-
485981
Streptomyces bluensis
L-ornithine + 1-guanidino-1-deoxy-scyllo-inositol 4-phosphate
-
-
-
?
L-arginine + 1-amino-1-deoxy-scyllo-inositol-4-phosphate
-
485981
Streptomyces kanamyceticus
L-ornithine + 1-guanidino-1-deoxy-scyllo-inositol 4-phosphate
-
-
-
?
L-arginine + 1-amino-1-deoxy-scyllo-inositol-4-phosphate
-
485981
Streptomyces griseus W4
L-ornithine + 1-guanidino-1-deoxy-scyllo-inositol 4-phosphate
-
-
-
?
streptidine phosphate + glycylglycine
-
485981
Streptomyces bikiniensis
?
-
-
-
?
streptidine phosphate + glycylglycine
-
485981
Streptomyces griseocarneus
?
-
-
-
?
streptidine phosphate + glycylglycine
-
485981
Streptomyces bluensis
?
-
-
-
?
streptidine phosphate + glycylglycine
-
485981
Streptomyces kanamyceticus
?
-
-
-
?
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-arginine + 1-amino-1-deoxy-scyllo-inositol 4-phosphate
Streptomyces griseus
-
L-ornithine + 1-guanidino-1-deoxy-scyllo-inositol 4-phosphate
-
-
?
L-arginine + 1-amino-1-deoxy-scyllo-inositol 4-phosphate
Streptomyces griseocarneus
-
L-ornithine + 1-guanidino-1-deoxy-scyllo-inositol 4-phosphate
-
-
?
L-arginine + 1-amino-1-deoxy-scyllo-inositol 4-phosphate
Streptomyces bluensis
-
L-ornithine + 1-guanidino-1-deoxy-scyllo-inositol 4-phosphate
-
-
?
L-arginine + 1-amino-1-deoxy-scyllo-inositol 4-phosphate
Streptomyces kanamyceticus
-
L-ornithine + 1-guanidino-1-deoxy-scyllo-inositol 4-phosphate
-
-
?
L-arginine + 1-amino-1-deoxy-scyllo-inositol 4-phosphate
Streptomyces bikiniensis
biosynthesis of streptomycin
L-ornithine + 1-guanidino-1-deoxy-scyllo-inositol 4-phosphate
-
-
?
L-arginine + 1-amino-1-deoxy-scyllo-inositol 4-phosphate
Streptomyces griseus W4
-
L-ornithine + 1-guanidino-1-deoxy-scyllo-inositol 4-phosphate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Streptomyces bluensis
-
Streptomyces bikiniensis
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Streptomyces kanamyceticus
-
mycelium
-
Streptomyces griseocarneus
-
mycelium
-
Streptomyces bikiniensis
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
arginine + 2-deoxystreptamine 6-phosphate
-
485981
Streptomyces bikiniensis
N-amidino-2-deoxystreptamine 6-phosphate + ornithine
-
-
-
?
arginine + 2-deoxystreptamine 6-phosphate
-
485981
Streptomyces bluensis
N-amidino-2-deoxystreptamine 6-phosphate + ornithine
-
-
-
?
arginine + scyllo-inosamine 4-phosphate
-
485981
Streptomyces bikiniensis
ornithine + N-amidino-scyllo-inosamine-4-phosphate
-
-
-
?
arginine + scyllo-inosamine 4-phosphate
reversibility of transamidinations with the monoamidinated inosamine phosphate has not yet been demonstrated
485981
Streptomyces bluensis
ornithine + N-amidino-scyllo-inosamine-4-phosphate
-
-
-
?
arginine + streptamine 6-phosphate
-
485981
Streptomyces bluensis
ornithine + N-amidinostreptamine 6-phosphate
-
-
-
?
L-arginine + 1-amino-1-deoxy-scyllo-inositol 4-phosphate
-
485981
Streptomyces griseus
L-ornithine + 1-guanidino-1-deoxy-scyllo-inositol 4-phosphate
-
-
-
?
L-arginine + 1-amino-1-deoxy-scyllo-inositol 4-phosphate
-
485981
Streptomyces griseocarneus
L-ornithine + 1-guanidino-1-deoxy-scyllo-inositol 4-phosphate
-
-
-
?
L-arginine + 1-amino-1-deoxy-scyllo-inositol 4-phosphate
-
485981
Streptomyces bluensis
L-ornithine + 1-guanidino-1-deoxy-scyllo-inositol 4-phosphate
-
-
-
?
L-arginine + 1-amino-1-deoxy-scyllo-inositol 4-phosphate
-
485981
Streptomyces kanamyceticus
L-ornithine + 1-guanidino-1-deoxy-scyllo-inositol 4-phosphate
-
-
-
?
L-arginine + 1-amino-1-deoxy-scyllo-inositol 4-phosphate
biosynthesis of streptomycin
485981
Streptomyces bikiniensis
L-ornithine + 1-guanidino-1-deoxy-scyllo-inositol 4-phosphate
-
-
-
?
L-arginine + 1-amino-1-deoxy-scyllo-inositol 4-phosphate
-
485981
Streptomyces griseus W4
L-ornithine + 1-guanidino-1-deoxy-scyllo-inositol 4-phosphate
-
-
-
?
L-arginine + 1-amino-1-deoxy-scyllo-inositol-4-phosphate
-
485981
Streptomyces griseus
L-ornithine + 1-guanidino-1-deoxy-scyllo-inositol 4-phosphate
-
-
-
?
L-arginine + 1-amino-1-deoxy-scyllo-inositol-4-phosphate
-
485981
Streptomyces bikiniensis
L-ornithine + 1-guanidino-1-deoxy-scyllo-inositol 4-phosphate
-
-
-
?
L-arginine + 1-amino-1-deoxy-scyllo-inositol-4-phosphate
-
485981
Streptomyces griseocarneus
L-ornithine + 1-guanidino-1-deoxy-scyllo-inositol 4-phosphate
-
-
-
?
L-arginine + 1-amino-1-deoxy-scyllo-inositol-4-phosphate
-
485981
Streptomyces bluensis
L-ornithine + 1-guanidino-1-deoxy-scyllo-inositol 4-phosphate
-
-
-
?
L-arginine + 1-amino-1-deoxy-scyllo-inositol-4-phosphate
-
485981
Streptomyces kanamyceticus
L-ornithine + 1-guanidino-1-deoxy-scyllo-inositol 4-phosphate
-
-
-
?
L-arginine + 1-amino-1-deoxy-scyllo-inositol-4-phosphate
-
485981
Streptomyces griseus W4
L-ornithine + 1-guanidino-1-deoxy-scyllo-inositol 4-phosphate
-
-
-
?
streptidine phosphate + glycylglycine
-
485981
Streptomyces bikiniensis
?
-
-
-
?
streptidine phosphate + glycylglycine
-
485981
Streptomyces griseocarneus
?
-
-
-
?
streptidine phosphate + glycylglycine
-
485981
Streptomyces bluensis
?
-
-
-
?
streptidine phosphate + glycylglycine
-
485981
Streptomyces kanamyceticus
?
-
-
-
?
Other publictions for EC 2.1.4.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
485977
Fritsche
The ligand-induced structural ...
Streptomyces griseus
J. Biol. Chem.
274
3026-3032
1999
-
-
-
-
-
-
-
-
-
-
2
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485976
Bedekar
-
The comparative amino acid seq ...
Streptomyces bluensis, Streptomyces consensus, Streptomyces galbus, Streptomyces glaucescens strB1, Streptomyces glaucescens strB2, Streptomyces griseus, Streptomyces griseus ATCC 10137, Streptomyces griseus SPH, Streptomyces griseus strB2
Comp. Biochem. Physiol. B
119B
677-690
1998
-
-
-
-
-
-
-
-
-
-
-
11
-
11
-
-
-
-
-
3
-
-
25
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
11
-
-
-
-
-
3
-
-
25
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485989
Fritsche
Crystal Structure of L-Arginin ...
Streptomyces galbus, Streptomyces galbus strB1, Streptomyces glaucescens strB1, Streptomyces glaucescens strB2, Streptomyces griseus, Streptomyces griseus strB1
Biochemistry
37
17664-17672
1998
-
-
1
1
-
-
-
-
-
-
-
8
-
7
-
-
1
-
-
2
-
-
28
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
8
-
-
-
1
-
2
-
-
28
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485973
Humm
-
Recombinant expression and iso ...
Streptomyces griseus
Biochem. J.
322
771-776
1997
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485971
Guthmiller
Cloning and sequencing of rat ...
Streptomyces griseus
J. Biol. Chem.
269
17556-17560
1994
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485985
Tohyama
Nucleotide sequence of the str ...
Streptomyces bikiniensis, Streptomyces griseus
Nucleic acids Res.
15
1819-1833
1987
-
-
-
-
-
-
-
-
-
-
1
2
-
4
-
-
-
-
-
1
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
1
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485984
Walker
L-Arginine:inosamine-P amindin ...
Streptomyces bikiniensis, Streptomyces galbus, Streptomyces griseocarneus, Streptomyces humidus, Streptomyces hygroscopicus, Streptomyces ornatus
Methods Enzymol.
43
451-458
1975
-
-
-
-
-
-
10
-
-
-
-
6
-
6
-
-
2
-
-
6
1
-
26
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
6
-
-
-
2
-
6
1
-
26
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485983
Walker
Biosynthesis of the monoguanid ...
Streptomyces hygroscopicus
J. Biol. Chem.
249
2397-2404
1974
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485964
Walker
-
Amidinotranferases ...
Streptomyces bikiniensis, Streptomyces bluensis, Streptomyces galbus, Streptomyces griseocarneus, Streptomyces griseus, Streptomyces humidus, Streptomyces hygroscopicus
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
9
497-509
1973
-
-
-
-
-
-
-
-
-
-
-
7
-
7
-
-
1
-
-
5
-
-
23
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
1
-
5
-
-
23
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
485981
Walker
Enzymic studies on the biosynt ...
no activity in Streptomyces griseus, no activity in Streptomyces griseus ATCC 10971, Streptomyces bikiniensis, Streptomyces bluensis, Streptomyces griseocarneus, Streptomyces griseus, Streptomyces griseus W4, Streptomyces kanamyceticus
J. Biol. Chem.
241
1262-1270
1966
-
-
-
-
-
-
-
-
-
-
-
6
-
8
-
-
2
-
-
3
-
-
21
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
2
-
3
-
-
21
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-