Literature summary for 2.1.3.3 extracted from

Xiong, X.; Anderson, P.M.
Purification and properties of ornithine carbamoyl transferase from liver of Squalus acanthias (1989), Arch. Biochem. Biophys., 270, 198-207.

Search for more literature for 2.1.3.3

Inhibitors

Inhibitors	Comment	Organism	Structure
citrulline	competitive to carbamoyl phosphate	Squalus acanthias
L-norvaline	-	Squalus acanthias
MgCl2	10 mM, 14% inhibition	Squalus acanthias
NaCl	50 mM, 25% inhibition	Squalus acanthias
ornithine	-	Squalus acanthias
phosphate	competitive vs. carbamoylphosphate	Squalus acanthias

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Squalus acanthias	5739	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
38000	-	3 * 38000, SDS-PAGE	Squalus acanthias
113000	-	gel filtration	Squalus acanthias

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
carbamoyl phosphate + L-ornithine	Squalus acanthias	catalyzes the last step of mitochondrial citrulline synthesis	phosphate + L-citrulline	-	?

Organism

Organism	UniProt	Comment	Textmining
Squalus acanthias	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
DEAE-Sephadex, hydoxyapatite C, delta-N-(phosphonacetyl)-L-ornithine affinity chromatography	Squalus acanthias

Reaction

Reaction	Comment	Organism	Reaction ID
carbamoyl phosphate + L-ornithine = phosphate + L-citrulline	mechanism	Squalus acanthias	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Squalus acanthias	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
27.1	-	-	Squalus acanthias

Storage Stability

Storage Stability	Organism
-20°C, 0.05 potassium phosphate buffer, pH 7.6, 1 month, no loss of activity	Squalus acanthias

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
carbamoyl phosphate + L-ornithine	-	Squalus acanthias	phosphate + L-citrulline	-	?
carbamoyl phosphate + L-ornithine	catalyzes the last step of mitochondrial citrulline synthesis	Squalus acanthias	phosphate + L-citrulline	-	?

Subunits

Subunits	Comment	Organism
trimer	3 * 38000, SDS-PAGE	Squalus acanthias

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.06	-	ornithine	-	Squalus acanthias

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Release 2023.1 (January 2023)