Any feedback?
Literature summary for 2.1.3.3 extracted from
- Ornithine transcarbamylase from Neurospora crassa: purification and properties (1985), Arch. Biochem. Biophys., 239, 172-183.
General Stability
| General Stability | Organism |
|---|---|
| glycerol, 20% v/v, stabilizes during freeze-thawing | Neurospora crassa |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
3 * 37000, SDS-PAGE | Neurospora crassa |
| 110000 | - |
SDS-PAGE of dimethyl suberimidate cross-linked enzyme | Neurospora crassa |
| 115000 | - |
gel filtration | Neurospora crassa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carbamoyl phosphate + L-ornithine | Neurospora crassa | enzyme is involved in the biosynthesis of arginine in many organisms and participates in the urea cycle of mammals | phosphate + L-citrulline | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neurospora crassa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, 70°C for 25 min, gel filtration, ion-exchange chromatography | Neurospora crassa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 251 | - |
- |
Neurospora crassa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carbamoyl phosphate + L-ornithine | - |
Neurospora crassa | phosphate + L-citrulline | - |
? | |
| carbamoyl phosphate + L-ornithine | enzyme is involved in the biosynthesis of arginine in many organisms and participates in the urea cycle of mammals | Neurospora crassa | phosphate + L-citrulline | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 37000, SDS-PAGE | Neurospora crassa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Neurospora crassa |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
