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Literature summary for 2.1.3.3 extracted from
- Ornithine transcarbamylase from Salmonella typhimurium: purification, subunit composition, kinetic analysis, and immunological cross-reactivity (1977), J. Bacteriol., 129, 1387-1396.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| arginine | 5 mM, 42% inhibition | Salmonella enterica subsp. enterica serovar Typhimurium |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
3 * 39000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
| 116000 | - |
sucrose density gradient ultracentrifugation | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
- |
- |
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| protamine sulfate, ammonium sulfate, diethylaminoethyl-cellulose, Sephadex G-150, hydroxylapatite | Salmonella enterica subsp. enterica serovar Typhimurium |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| carbamoyl phosphate + L-ornithine = phosphate + L-citrulline | mechanism | Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1901 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carbamoyl phosphate + L-ornithine | - |
Salmonella enterica subsp. enterica serovar Typhimurium | phosphate + L-citrulline | - |
? | |
| carbamoyl phosphate + L-ornithine | - |
Pisum sativum | phosphate + L-citrulline | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | - |
Pisum sativum |
| trimer | 3 * 39000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
| 8 | - |
- |
Pisum sativum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | 50% of maximal activity at pH 9.0, 70% at pH 7.0 | Salmonella enterica subsp. enterica serovar Typhimurium |
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