Literature summary for 2.1.3.2 extracted from

Ruiz-Ramos, A.; Lallous, N.; Grande-Garcia, A.; Ramon-Maiques, S.
Expression, purification, crystallization and preliminary X-ray diffraction analysis of the aspartate transcarbamoylase domain of human CAD (2013), Acta Crystallogr. Sect. F, 69, 1425-1430.

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of the enzyme as part of the N-terminal His6-tagged maltose-binding fusion protein CAD complex in Escherichia coli strain BL21(DE3) pLysS cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified detagged isolated recombinant huATCase, free or in complex with inhibitor N-phosphonoacetyl-L-aspartate, 1.5 mg/ml protein, 0.1 M Tris pH 8.5, 6% PEG 8000, and 9% ethylene glycol is mixed with 0.1 M CAPS pH 10, 150 mM NaCl or 1.5 mg ml1 and 10 mM ZnCl2, 15% PEG 6000, 50 mM sodium acetate pH 4.8, and 0.5 mM inhibitor, X-ray diffraction structure determination and analysis at 2.1 A resolution	Homo sapiens

Crystallization (Comment)

Organism

purified detagged isolated recombinant huATCase, free or in complex with inhibitor N-phosphonoacetyl-L-aspartate, 1.5 mg/ml protein, 0.1 M Tris pH 8.5, 6% PEG 8000, and 9% ethylene glycol is mixed with 0.1 M CAPS pH 10, 150 mM NaCl or 1.5 mg ml1 and 10 mM ZnCl2, 15% PEG 6000, 50 mM sodium acetate pH 4.8, and 0.5 mM inhibitor, X-ray diffraction structure determination and analysis at 2.1 A resolution

Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
N-phosphonoacetyl-L-aspartate	binding structure, overview	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
34800	-	3 * 34800, detagged isolated recombinant enzyme, SDS-PAGE	Homo sapiens
102000	-	detagged isolated recombinant enzyme, gel filtration	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P27708	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from the N-terminal His6-tagged maltose-binding fusion protein CAD complex from Escherichia coli strain BL21(DE3) pLysS cells by ncikel affinity chromatography, ion-exchange chromatography, tag cleavage, and gel filtration. Three extra residues are left at the N-terminus of huATCase (GPM1915SP) after removal of the tag	Homo sapiens

Subunits

Subunits	Comment	Organism
homotrimer	3 * 34800, detagged isolated recombinant enzyme, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
aspartate transcarbamoylase	-	Homo sapiens
ATCase	-	Homo sapiens

General Information

General Information	Comment	Organism
evolution	in animals, CPSase, ATCase and DHOase are part of a 243 kDa multifunctional polypeptide named CAD	Homo sapiens
physiological function	upregulation of CAD, comprising CPSase, ATCase and DHOase activities, is essential for normal and tumour cell proliferation	Homo sapiens