Literature summary for 2.1.3.2 extracted from

Vitali, J.; Singh, A.K.; Soares, A.S.; Colaneri, M.J.
Structure of the catalytic chain of Methanococcus jannaschii aspartate transcarbamoylase in a hexagonal crystal form: insights into the path of carbamoyl phosphate to the active site of the enzyme (2012), Acta Crystallogr. Sect. F, 68, 527-534.

Cloned(Commentary)

Cloned (Comment)	Organism
expression of the catalytic trimer in Escherichia coli strain EK1911, which has a deletion in the pyrBI region of the chromosome and contains plasmids pEK406 coding for the Methanococcus jannaschii ATCase catalytic chain and pSJS1240	Methanocaldococcus jannaschii
expression of the regulatiry subunit in Escherichia coli ATCase-deficient derivative strain C600 which contains T7 RNA polymerase gene under lacUV5 control as a lambda lysogen	Methanocaldococcus jannaschii

Crystallization (Commentary)

Crystallization (Comment)	Organism
catalytic chain in the presence of the regulatory chain in the hexagonal space group P6322, with one monomer per asymmetric unit, sitting drop vapor diffusion method, mixing of 0.0013 protein-ligand solution, containing 11 mg/ml protein in 50 mM Tris, pH 8.3, 150 mM NaCl, 2 mM BME, 0.05 mM zinc acetate, with 0.001 ml of reservoir solution containing 2.0 M ammonium sulfate, 0.2 M potassium sodium tartrate tetrahydrate, 0.1 M sodium citrate tribasic dihydrate, pH 5.6, or 2.0 M ammonium sulfate, 0.2 M potassium sodium tartrate tetrahydrate, 0.1 M Tris-HCl, pH 7.5, 22°C, X-ray diffraction structure determination and analysis at 2.50 A resolution, molecular replacement	Methanocaldococcus jannaschii

Crystallization (Comment)

Organism

catalytic chain in the presence of the regulatory chain in the hexagonal space group P6322, with one monomer per asymmetric unit, sitting drop vapor diffusion method, mixing of 0.0013 protein-ligand solution, containing 11 mg/ml protein in 50 mM Tris, pH 8.3, 150 mM NaCl, 2 mM BME, 0.05 mM zinc acetate, with 0.001 ml of reservoir solution containing 2.0 M ammonium sulfate, 0.2 M potassium sodium tartrate tetrahydrate, 0.1 M sodium citrate tribasic dihydrate, pH 5.6, or 2.0 M ammonium sulfate, 0.2 M potassium sodium tartrate tetrahydrate, 0.1 M Tris-HCl, pH 7.5, 22°C, X-ray diffraction structure determination and analysis at 2.50 A resolution, molecular replacement

Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-aspartate + carbamoyl phosphate	Methanocaldococcus jannaschii	-	phosphate + N-carbamoyl-L-aspartate	-	?

Organism

Organism	UniProt	Comment	Textmining
Methanocaldococcus jannaschii	Q58801	regulatory subunit	-
Methanocaldococcus jannaschii	Q58976	catalytic subunit trimer	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-aspartate + carbamoyl phosphate	-	Methanocaldococcus jannaschii	phosphate + N-carbamoyl-L-aspartate	-	?

Synonyms

Synonyms	Comment	Organism
aspartate transcarbamoylase	-	Methanocaldococcus jannaschii
ATCase	-	Methanocaldococcus jannaschii