Activating Compound | Comment | Organism | Structure |
---|---|---|---|
4-aminobutyrate | 400% activation | Enterococcus faecalis | |
beta-Alanine | 600% activation | Enterococcus faecalis | |
Butyrate | 5700% activation | Enterococcus faecalis | |
Carbamoyl-beta-alanine | 5000% activation | Enterococcus faecalis | |
D-Aspartate | 5700% activation | Enterococcus faecalis | |
formate | activates | Enterococcus faecalis | |
Isobutyrate | 5800% activation | Enterococcus faecalis | |
L-2-aminobutyrate | 1400% activation | Enterococcus faecalis | |
L-alanine | 800% activation | Enterococcus faecalis | |
additional information | every type of anion tested, including ATP and CTP can stimulate the reaction to a certain extent | Enterococcus faecalis | |
propionate | 5600% activation | Enterococcus faecalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
HgCl2 | 0.001 mM, 50% inhibition | Enterococcus faecalis | |
Mersalyl | 0.01 mM, 50% inhibition | Enterococcus faecalis | |
p-mercuribenzoate | 0.01 mM, 50% inhibition | Enterococcus faecalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
Carbamoyl phosphate | at high salt concentrations | Enterococcus faecalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32500 | - |
4 * 32500, SDS-PAGE | Enterococcus faecalis |
125000 | - |
sucrose density gradient centrifugation | Enterococcus faecalis |
128000 | - |
sedimentation equilibrium | Enterococcus faecalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbamoylphosphate + L-aspartate | Enterococcus faecalis | - |
phosphate + N-carbamoyl-L-aspartate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
pH 4.8, hydroxylapatite, ammonium sulfate, DEAE-Sephadex | Enterococcus faecalis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate | reaction mechanism | Enterococcus faecalis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
350 | - |
- |
Enterococcus faecalis |
Storage Stability | Organism |
---|---|
-20°C, 0.1-1 mg protein/ml, at least 2 years, no loss of activity 4°C, 0.1-1 mg/ml, at least 2 months, no loss of activity | Enterococcus faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbamoyl phosphate + L-aspartate | - |
Enterococcus faecalis | phosphate + N-carbamoyl-L-aspartate | - |
? | |
carbamoylphosphate + L-aspartate | - |
Enterococcus faecalis | phosphate + N-carbamoyl-L-aspartate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 32500, SDS-PAGE | Enterococcus faecalis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.6 | - |
stable | Enterococcus faecalis |