BRENDA - Enzyme Database
show all sequences of 2.1.3.15

The structure of the carboxyltransferase component of acetyl-CoA carboxylase reveals a zinc-binding motif unique to the bacterial enzyme

Bilder, P.; Lightle, S.; Bainbridge, G.; Ohren, J.; Finzel, B.; Sun, F.; Holley, S.; Al-Kassim, L.; Spessard, C.; Melnick, M.; Newcomer, M.; Waldrop, G.; Biochemistry 45, 1712-1722 (2006)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BL21-Gold (DE3) and Escherichia coli BL21(DE3)pLysS cells
Staphylococcus aureus
expressed in Escherichia coli BL21-Gold (DE3) and Escherichia coli BL21(DE3)pLysS cells
Escherichia coli
Crystallization (Commentary)
Crystallization (Commentary)
Organism
vapor diffusion method, using 1 M NaH2PO4, 1 M K2HPO4, 0.2 M Li2SO4, and 0.1 M CAPS at pH 9.5
Escherichia coli
vapor diffusion method, using 30% (w/v) PEG-400, 0.1 M HEPES at pH 8.0, and 0.2 M MgCl2
Staphylococcus aureus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
[biotin carboxyl-carrier protein]-N6-carboxybiotinyl-L-lysine + acetyl-CoA
Staphylococcus aureus
-
[biotin carboxyl-carrier protein]-N6-biotinyl-L-lysine + malonyl-CoA
-
-
?
[biotin carboxyl-carrier protein]-N6-carboxybiotinyl-L-lysine + acetyl-CoA
Escherichia coli
-
[biotin carboxyl-carrier protein]-N6-biotinyl-L-lysine + malonyl-CoA
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Escherichia coli
P0ABD5
-
-
Staphylococcus aureus
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
Ni-NTA resin column chromatography and Superdex 200 gel filtration
Staphylococcus aureus
Ni-NTA resin column chromatography, Hi-Trap column chromatography, and Superdex 200 gel filtration
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
[biotin carboxyl-carrier protein]-N6-carboxybiotinyl-L-lysine + acetyl-CoA
-
744305
Staphylococcus aureus
[biotin carboxyl-carrier protein]-N6-biotinyl-L-lysine + malonyl-CoA
-
-
-
?
[biotin carboxyl-carrier protein]-N6-carboxybiotinyl-L-lysine + acetyl-CoA
-
744305
Escherichia coli
[biotin carboxyl-carrier protein]-N6-biotinyl-L-lysine + malonyl-CoA
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
acetyl-CoA carboxylase carboxyltransferase domain
-
Staphylococcus aureus
acetyl-CoA carboxylase carboxyltransferase domain
-
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21-Gold (DE3) and Escherichia coli BL21(DE3)pLysS cells
Staphylococcus aureus
expressed in Escherichia coli BL21-Gold (DE3) and Escherichia coli BL21(DE3)pLysS cells
Escherichia coli
Crystallization (Commentary) (protein specific)
Crystallization
Organism
vapor diffusion method, using 1 M NaH2PO4, 1 M K2HPO4, 0.2 M Li2SO4, and 0.1 M CAPS at pH 9.5
Escherichia coli
vapor diffusion method, using 30% (w/v) PEG-400, 0.1 M HEPES at pH 8.0, and 0.2 M MgCl2
Staphylococcus aureus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
[biotin carboxyl-carrier protein]-N6-carboxybiotinyl-L-lysine + acetyl-CoA
Staphylococcus aureus
-
[biotin carboxyl-carrier protein]-N6-biotinyl-L-lysine + malonyl-CoA
-
-
?
[biotin carboxyl-carrier protein]-N6-carboxybiotinyl-L-lysine + acetyl-CoA
Escherichia coli
-
[biotin carboxyl-carrier protein]-N6-biotinyl-L-lysine + malonyl-CoA
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA resin column chromatography and Superdex 200 gel filtration
Staphylococcus aureus
Ni-NTA resin column chromatography, Hi-Trap column chromatography, and Superdex 200 gel filtration
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
[biotin carboxyl-carrier protein]-N6-carboxybiotinyl-L-lysine + acetyl-CoA
-
744305
Staphylococcus aureus
[biotin carboxyl-carrier protein]-N6-biotinyl-L-lysine + malonyl-CoA
-
-
-
?
[biotin carboxyl-carrier protein]-N6-carboxybiotinyl-L-lysine + acetyl-CoA
-
744305
Escherichia coli
[biotin carboxyl-carrier protein]-N6-biotinyl-L-lysine + malonyl-CoA
-
-
-
?
Other publictions for EC 2.1.3.15
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
758056
Shivaiah
Non-catalytic subunits facili ...
Arabidopsis thaliana
Plant Physiol.
182
756-775
2020
1
-
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1
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1
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1
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758050
Keereetaweep
Biotin attachment domain-cont ...
Arabidopsis thaliana
Plant Physiol.
177
208-215
2018
-
-
-
-
-
-
3
-
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1
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1
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1
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744173
Evans
Acetyl-CoA carboxylase from E ...
Escherichia coli
Arch. Biochem. Biophys.
636
100-109
2017
-
-
1
-
-
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3
-
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1
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1
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1
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2
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1
1
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3
2
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1
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1
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1
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758409
Pawelczyk
The influence of AccD5 on Acc ...
Mycobacterium tuberculosis, Mycolicibacterium smegmatis, Mycobacterium tuberculosis H37Rv, Mycolicibacterium smegmatis mc(2)155
Sci. Rep.
7
42692
2017
-
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4
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194
-
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4
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5
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4
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756040
Silvers
Crystal structure of carboxyl ...
Staphylococcus aureus
Biochemistry
55
4666-4674
2016
-
-
1
1
-
-
5
-
-
-
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2
-
1
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1
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3
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1
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1
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1
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5
1
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1
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2
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744336
Broussard
Structural analysis of substr ...
Haemophilus influenzae
Biochemistry
54
3860-3870
2015
-
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1
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1
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1
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744496
Raymer
Synthesis and characterizatio ...
Homo sapiens
Bioorg. Med. Chem. Lett.
19
2804-2807
2009
-
-
-
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-
1
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1
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1
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1
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744305
Bilder
The structure of the carboxyl ...
Staphylococcus aureus, Escherichia coli
Biochemistry
45
1712-1722
2006
-
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2
2
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2
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2
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2
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745266
Blanchard
The biotin domain peptide fro ...
Escherichia coli
J. Biol. Chem.
274
31767-31769
1999
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2
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