Cloned (Comment) | Organism |
---|---|
overexpression in Streptomyces lividans as an N-terminal His6 fusion protein | Streptomyces niveus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0019 | - |
novclobiocin 104 | pH 8.0, 30°C | Streptomyces niveus | |
0.0024 | - |
decarbamoylnovobiocin | pH 8.0, 30°C | Streptomyces niveus | |
0.0143 | - |
novclobiocin 105 | pH 8.0, 30°C | Streptomyces niveus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the enzyme is strictly dependent on the presence ATP and divalent cations such as Mg2+ or Mn2+. Optimal concentrations of both Mg2+ and ATP is 2 mM | Streptomyces niveus | |
Mn2+ | the enzyme is strictly dependent on the presence ATP and divalent cations such as Mg2+ or Mn2+. Optimal concentrations of both Mg2+ and ATP is 2 mM | Streptomyces niveus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78500 | - |
1 * 78500, calculated from sequence | Streptomyces niveus |
79000 | - |
gel filtration | Streptomyces niveus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbamoyl phosphate + decarbamoylnovobiocin | Streptomyces niveus | the enzyme is involved in the biosynthesis of the aminocoumarin antibiotics, novobiocin | phosphate + novobiocin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces niveus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
N-terminal His6 fusion protein | Streptomyces niveus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbamoyl phosphate + decarbamoylnovobiocin | the enzyme is involved in the biosynthesis of the aminocoumarin antibiotics, novobiocin | Streptomyces niveus | phosphate + novobiocin | - |
? | |
carbamoyl phosphate + decarbamoylnovobiocin | NovN is specific for carbamoyl phosphate as substrate. With acetyl phosphate, no product formation is observed | Streptomyces niveus | phosphate + novobiocin | - |
? | |
carbamoyl phosphate + novclobiocin 104 | 87% of novclobiocin 104 is converted to the carbamoylated derivative after overnight incubation | Streptomyces niveus | phosphate + ? | - |
? | |
carbamoyl phosphate + novclobiocin 105 | - |
Streptomyces niveus | phosphate + novclobiocin 115 | - |
? | |
carbamoyl phosphate + novclobiocin 107 | - |
Streptomyces niveus | phosphate + novclobiocin 117 | - |
? | |
carbamoyl phosphate + novclobiocin 108 | - |
Streptomyces niveus | phosphate + novclobiocin 118 | - |
? | |
carbamoyl phosphate + novclobiocin 283 | - |
Streptomyces niveus | phosphate + novclobiocin 284 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 78500, calculated from sequence | Streptomyces niveus |
Synonyms | Comment | Organism |
---|---|---|
NovN | - |
Streptomyces niveus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Streptomyces niveus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.32 | - |
novclobiocin 104 | pH 8.0, 30°C | Streptomyces niveus | |
1.53 | - |
novclobiocin 105 | pH 8.0, 30°C | Streptomyces niveus | |
2.4 | - |
decarbamoylnovobiocin | pH 8.0, 30°C | Streptomyces niveus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Streptomyces niveus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | the enzyme is strictly dependent on the presence ATP and divalent cations such as Mg2+ or Mn2+. Optimal concentrations of both Mg2+ and ATP is 2 mM | Streptomyces niveus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the biosynthesis of the aminocoumarin antibiotics, novobiocin | Streptomyces niveus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
107 | - |
novclobiocin 105 | pH 8.0, 30°C | Streptomyces niveus | |
695 | - |
novclobiocin 104 | pH 8.0, 30°C | Streptomyces niveus | |
1000 | - |
decarbamoylnovobiocin | pH 8.0, 30°C | Streptomyces niveus |