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Literature summary for 2.1.3.11 extracted from

  • Shi, D.; Yu, X.; Cabrera-Luque, J.; Chen, T.Y.; Roth, L.; Morizono, H.; Allewell, N.M.; Tuchman, M.
    A single mutation in the active site swaps the substrate specificity of N-acetyl-L-ornithine transcarbamylase and N-succinyl-L-ornithine transcarbamylase (2007), Protein Sci., 16, 1689-1699.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.3.11

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant P90E, in complex with carbamoyl phosphate and N-acetyl-L-norvaline or N-succinyl-L-norvaline Bacteroides fragilis
mutants E92P, E92S, E92V, E92A, in complex with carbamoyl phosphate and N-acetyl-L-norvaline or N-succinyl-L-norvaline Xanthomonas campestris

Protein Variants

Protein Variants Comment Organism
E92A site-directed mutagenesi of N-acetyl-L-ornithine carbamoyltransferase, conversion to N-succinly-L-ornithine carbamoyltransferase Xanthomonas campestris
E92P site-directed mutagenesi of N-acetyl-L-ornithine carbamoyltransferase, conversion to N-succinly-L-ornithine carbamoyltransferase Xanthomonas campestris
E92S site-directed mutagenesi of N-acetyl-L-ornithine carbamoyltransferase, conversion to N-succinly-L-ornithine carbamoyltransferase Xanthomonas campestris
E92V site-directed mutagenesi of N-acetyl-L-ornithine carbamoyltransferase, conversion to N-succinly-L-ornithine carbamoyltransferase Xanthomonas campestris
P90E conversion of N-succinly-L-ornithine carbamoyltransferase to N-acetyl-L-ornithine carbamoyltransferase Bacteroides fragilis

Organism

Organism UniProt Comment Textmining
Bacteroides fragilis
-
-
-
Xanthomonas campestris
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.21
-
 mutant E92P of N-acetyl-L-ornithine carbamoyltransferase, pH 8.3, 25°C Xanthomonas campestris
29.8
-
 mutant E92A of N-acetyl-L-ornithine carbamoyltransferase, pH 8.3, 25°C Xanthomonas campestris
40.5
-
 mutant E92V of N-acetyl-L-ornithine carbamoyltransferase, pH 8.3, 25°C Xanthomonas campestris
66.9
-
 mutant E92S, pH 8.3, 25°C Xanthomonas campestris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-L-ornithine + carbamoyl phosphate no substrate for wild-type, but for mutant P90E/T242L Xanthomonas campestris N-acetyl-L-citrulline + phosphate
-
 ?
N-succinyl-L-ornithine + carbamoyl phosphate
-
 Xanthomonas campestris N-succinyl-L-citrulline + phosphate
-
 ?