Crystallization (Comment) | Organism |
---|---|
in complex with carbamyl phosphate and N-succinyl-L-norvaline, as well as sulfate and N-succinyl-L-norvaline, diffraction to 2.9 and 2.8 A resolution, respectively | Bacteroides fragilis |
Protein Variants | Comment | Organism |
---|---|---|
P90E/T242L | contrary to wild-type, mutant accepts N-acetyl-L-ornithine as a substrate. Decrease in Km value for N-succinyl-L-ornithine | Bacteroides fragilis |
T242L | slight decrease in Km value | Bacteroides fragilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
N-succinyl-L-ornithine | mutant P90E/T242L, pH 8.3, 25°C | Bacteroides fragilis | |
0.0119 | - |
N-acetyl-L-ornithine | mutant P90E/T242L, pH 8.3, 25°C | Bacteroides fragilis | |
0.195 | - |
N-succinyl-L-ornithine | mutant T242L, pH 8.3, 25°C | Bacteroides fragilis | |
0.238 | - |
N-succinyl-L-ornithine | wild-type, pH 8.3, 25°C | Bacteroides fragilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bacteroides fragilis | Bacteroides fragilis uses N-succinyl-L-ornithine rather than N-acetyl-L-ornithine for de novo arginine biosynthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacteroides fragilis | Q8A1E9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bacteroides fragilis uses N-succinyl-L-ornithine rather than N-acetyl-L-ornithine for de novo arginine biosynthesis | Bacteroides fragilis | ? | - |
? | |
additional information | no substrate: L-ornithine | Bacteroides fragilis | ? | - |
? | |
N-acetyl-L-ornithine + carbamoyl phosphate | no substrate for wild-type, but for mutant P90E/T242L | Bacteroides fragilis | N-acetyl-L-citrulline + phosphate | - |
? | |
N-succinyl-L-ornithine + carbamoyl phosphate | - |
Bacteroides fragilis | N-succinyl-L-citrulline + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ArgF | - |
Bacteroides fragilis |