Literature summary for 2.1.2.9 extracted from

Gite, S.; Li, Y.; Ramesh, V.; RajBhandary, U.L.
Escherichia coli methionyl-tRNA formyltransferase: Role of amino acids conserved in the linker region and in the C-terminal domain on the specific recognition of the initiator tRNA (2000), Biochemistry, 39, 2218-2226.

Cloned(Commentary)

Cloned (Comment)	Organism
gene encoding MTF is cloned and sequenced, expression of wild-type and mutant enzymes in Escherichia coli	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structure of enzyme complexed with formylmethionyl-tRNAfMet	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
additional information	several C-terminal deletion mutants and substitution mutants in the linker region between the C- and N-terminal domain and the C-terminal domain	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
additional information	-	additional information	values for several mutant enzymes in the linker region and C-terminal domain	Escherichia coli
0.0005	-	E. coli L-methionyl-tRNAfMet	-	Escherichia coli
0.0037	-	L-methionyl-tRNAfMet	MTFDeltaC20	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O	Escherichia coli	involved in initiation of protein biosynthesis	tetrahydrofolate + N-formylmethionyl-tRNAfMet	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P23882	-	-
Escherichia coli JM109	P23882	-	-
Escherichia coli XL1-Blue	P23882	-	-

Purification (Commentary)

Purification (Comment)	Organism
purification of His-tagged wild-type and C-terminal deletion mutant recombinant enzymes, expressed in Escherichia coli	Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
10-formyltetrahydrofolate + E. coli L-methionyl-tRNAfMet	-	Escherichia coli	tetrahydrofolate + E. coli N-formylmethionyl-tRNAfMet	-	r
10-formyltetrahydrofolate + L-methionyl-tRNAfMet	-	Escherichia coli	tetrahydrofolate + N-formylmethionyl-tRNAfMet	-	r
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O	role of the linker region between N- and C-terminal domain	Escherichia coli	tetrahydrofolate + N-formylmethionyl-tRNAfMet	-	?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O	specificity determinants at the end of the acceptor stem of tRNAfMet	Escherichia coli	tetrahydrofolate + N-formylmethionyl-tRNAfMet	-	?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O	C-terminal region is very important for enzyme activity: basic amino acids contribute mostly toward the nonspecific binding and orientation of the tRNA 3'-end toward the catalytic site	Escherichia coli	tetrahydrofolate + N-formylmethionyl-tRNAfMet	-	?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O	specificity is restricted to methionyl-tRNAfMet	Escherichia coli	tetrahydrofolate + N-formylmethionyl-tRNAfMet	-	?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O	recognition mechanism of methionyl-tRNAfMet	Escherichia coli	tetrahydrofolate + N-formylmethionyl-tRNAfMet	-	?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O	important role of a 16-amino acid insertion loop in enzyme for tRNA recognition	Escherichia coli	tetrahydrofolate + N-formylmethionyl-tRNAfMet	-	?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O	involved in initiation of protein biosynthesis	Escherichia coli	tetrahydrofolate + N-formylmethionyl-tRNAfMet	-	?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O	role of the linker region between N- and C-terminal domain	Escherichia coli JM109	tetrahydrofolate + N-formylmethionyl-tRNAfMet	-	?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O	specificity determinants at the end of the acceptor stem of tRNAfMet	Escherichia coli JM109	tetrahydrofolate + N-formylmethionyl-tRNAfMet	-	?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O	C-terminal region is very important for enzyme activity: basic amino acids contribute mostly toward the nonspecific binding and orientation of the tRNA 3'-end toward the catalytic site	Escherichia coli JM109	tetrahydrofolate + N-formylmethionyl-tRNAfMet	-	?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O	role of the linker region between N- and C-terminal domain	Escherichia coli XL1-Blue	tetrahydrofolate + N-formylmethionyl-tRNAfMet	-	?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O	specificity determinants at the end of the acceptor stem of tRNAfMet	Escherichia coli XL1-Blue	tetrahydrofolate + N-formylmethionyl-tRNAfMet	-	?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O	C-terminal region is very important for enzyme activity: basic amino acids contribute mostly toward the nonspecific binding and orientation of the tRNA 3'-end toward the catalytic site	Escherichia coli XL1-Blue	tetrahydrofolate + N-formylmethionyl-tRNAfMet	-	?
additional information	no formylation of methionyl-tRNAmMet	Escherichia coli	?	-	?
additional information	enzyme structure	Escherichia coli	?	-	?
additional information	no formylation of methionyl-tRNAmMet	Escherichia coli JM109	?	-	?
additional information	enzyme structure	Escherichia coli JM109	?	-	?
additional information	no formylation of methionyl-tRNAmMet	Escherichia coli XL1-Blue	?	-	?
additional information	enzyme structure	Escherichia coli XL1-Blue	?	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
additional information	-	additional information	values for several mutant enzymes in the linker region and C-terminal domain	Escherichia coli
0.2	-	L-methionyl-tRNAfMet	MTFDeltaC20	Escherichia coli
37.3	-	L-methionyl-tRNAfMet	wild-type enzyme	Escherichia coli