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Literature summary for 2.1.2.9 extracted from

  • Li, Y.; Ramesh, V.; Mangroo, D.; Taneja, C.; RajBhandary, U.L.
    Suppressor mutations in Escherichia coli methionyl-tRNA formyltransferase that compensate for the formylation defect of a mutant tRNA aminoacylated with lysine (2000), Biochemistry, 39, 8039-8046.
    View publication on PubMed
Search for more literature for 2.1.2.9

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant fmt gene encoding MTF is cloned, overexpression of wild-type enzyme in Escherichia coli Novablue Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of enzyme complexed with formylmethionyl-tRNAfMet Escherichia coli

Protein Variants

Protein Variants Comment Organism
A55T no suppressor mutation Escherichia coli
G178D suppressor mutation, compensates for the formylation defect of a mutant elongator lysine-tRNAmMet, it compensates for lysine that is attached to the tRNA, G178D, G178K and G178L are progressively weaker suppressors than G178E Escherichia coli
G178E suppressor mutation, compensates for the formylation defect of a mutant elongator lysine-tRNAmMet, G178D, G178K and G178L are progressively weaker suppressors Escherichia coli
G178K suppressor mutation, compensates for the formylation defect of a mutant elongator lysine-tRNAmMet, G178D, G178K and G178L are progressively weaker suppressors than G178E Escherichia coli
G178L suppressor mutation, compensates for the formylation defect of a mutant elongator lysine-tRNAmMet, G178D, G178K and G178L are progressively weaker suppressors than G178E Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00051
-
 L-methionyl-tRNAfMet wild-type enzyme Escherichia coli
0.00072
-
 L-methionyl-tRNAfMet G178E mutant enzyme Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O Escherichia coli involved in initiation of protein biosynthesis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O Escherichia coli JM109 involved in initiation of protein biosynthesis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O Escherichia coli CA274 involved in initiation of protein biosynthesis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli
-
 strains DH5Alpha, Novablue(DE3) pLysS
-
Escherichia coli CA274
-
-
-
Escherichia coli JM109
-
-
-

Purification (Commentary)

Purification (Comment) Organism
14fold purification of recombinant wild-type enzyme, overexpressed in Escherichia coli Novablue, purification of His-tagged G178E mutant enzyme Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-formyltetrahydrofolate + L-methionyl-tRNAfMet
-
 Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 r
10-formyltetrahydrofolate + L-methionyl-tRNAfMet
-
 Escherichia coli JM109 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 r
10-formyltetrahydrofolate + L-methionyl-tRNAfMet
-
 Escherichia coli CA274 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 r
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O specificity is restricted to methionyl-tRNAfMet Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O involved in initiation of protein biosynthesis Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O specificity is restricted to methionyl-tRNAfMet Escherichia coli JM109 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O involved in initiation of protein biosynthesis Escherichia coli JM109 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O specificity is restricted to methionyl-tRNAfMet Escherichia coli CA274 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O involved in initiation of protein biosynthesis Escherichia coli CA274 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
additional information mutant tRNAs as poor substrates Escherichia coli ?
-
 ?
additional information enzyme structure Escherichia coli ?
-
 ?
additional information mutant tRNAs as poor substrates Escherichia coli JM109 ?
-
 ?
additional information enzyme structure Escherichia coli JM109 ?
-
 ?
additional information mutant tRNAs as poor substrates Escherichia coli CA274 ?
-
 ?
additional information enzyme structure Escherichia coli CA274 ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.9
-
 L-methionyl-tRNAfMet G178E mutant enzyme Escherichia coli
13.9
-
 L-methionyl-tRNAfMet wild-type enzyme Escherichia coli