Any feedback?
Literature summary for 2.1.2.9 extracted from
- Structural and sequence elements important for recognition of Escherichia coli formylmethionine tRNA by methionyl-tRNA transformylase are clustered in the acceptor stem (1991), J. Biol. Chem., 266, 18012-18017.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic parameters of the formylation of various mutant methionyl-tRNAs | Escherichia coli | |
| 0.0017 | - |
L-methionyl-tRNAfMet | - |
Escherichia coli |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 10-formyltetrahydrofolate + L-methionyl-tRNAfMet | - |
Escherichia coli | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
r | |
| 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | specificity determinants at the end of the acceptor stem of tRNAfMet | Escherichia coli | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
| 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | formylates methionyl-tRNAfMet from E. coli | Escherichia coli | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
| 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | specificity is restricted to methionyl-tRNAfMet | Escherichia coli | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
| 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | nucleotide-mutants of tRNAfMet as substrates | Escherichia coli | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 10-formyltetrahydrofolate | - |
Escherichia coli | |
| tetrahydrofolate | - |
Escherichia coli | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
