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Literature summary for 2.1.2.9 extracted from

  • Giege, R.; Ebel, J.P.; Clarke, B.F.C.
    Formylation of mischarged E. coli tRNA Met f (1973), FEBS Lett., 30, 291-295.
    View publication on PubMed
Search for more literature for 2.1.2.9

Inhibitors

Inhibitors Comment Organism Structure
tRNAfMet non-aminoacylated tRNAfMet is a competitive inhibitor Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0006
-
 L-methionyl-tRNAfMet
-
 Escherichia coli
0.0013
-
 phenylalanyl-tRNAfMet
-
 Escherichia coli
0.0014
-
 valyl-tRNAfMet
-
 Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Escherichia coli 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O Escherichia coli involved in initiation of protein biosynthesis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O Escherichia coli K12 MO involved in initiation of protein biosynthesis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 lot 15-290
-
Escherichia coli K12 MO
-
 lot 15-290
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-formyltetrahydrofolate + L-methionyl-tRNAfMet
-
 Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 r
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O formylates methionyl-tRNAfMet from E. coli Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O specific for methionyl-tRNAfMet, specificity depends exclusively on the nature of tRNA moiety of aminoacylated tRNAfMet Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O involved in initiation of protein biosynthesis Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O formylates methionyl-tRNAfMet from E. coli Escherichia coli K12 MO tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O specific for methionyl-tRNAfMet, specificity depends exclusively on the nature of tRNA moiety of aminoacylated tRNAfMet Escherichia coli K12 MO tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O involved in initiation of protein biosynthesis Escherichia coli K12 MO tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + phenylalanyl-tRNAfMet + H2O mischarged E. coli tRNAfMet, reduced velocity compared to the formylation of methionyl-tRNAfMet Escherichia coli tetrahydrofolate + N-formylphenylalanyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + phenylalanyl-tRNAfMet + H2O mischarged E. coli tRNAfMet, reduced velocity compared to the formylation of methionyl-tRNAfMet Escherichia coli K12 MO tetrahydrofolate + N-formylphenylalanyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + valyl-tRNAfMet + H2O mischarged E. coli tRNAfMet Escherichia coli tetrahydrofolate + N-formylvalyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + valyl-tRNAfMet + H2O reduced velocity compared to the formylation of methionyl-tRNAfMet Escherichia coli tetrahydrofolate + N-formylvalyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + valyl-tRNAfMet + H2O mischarged E. coli tRNAfMet Escherichia coli K12 MO tetrahydrofolate + N-formylvalyl-tRNAfMet
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
10-formyltetrahydrofolate
-
 Escherichia coli
tetrahydrofolate
-
 Escherichia coli