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Literature summary for 2.1.2.9 extracted from

  • Dickerman, H.W.; Steers, E.; Redfield, B.G.; Weissbach, H.
    Methionyl soluble ribonucleic acid transformylase. I. Purification and partial characterization (1967), J. Biol. Chem., 242, 1522-1525.
    View publication on PubMed
Search for more literature for 2.1.2.9

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 L-methionyl-tRNAMet
-
 Escherichia coli
0.0133
-
 10-formyltetrahydrofolate
-
 Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Escherichia coli 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ can partially replace Mg2+ Escherichia coli
K+ can partially replace Mg2+ Escherichia coli
Mg2+ requirement Escherichia coli
Mg2+ optimal concentration: 30 mM Escherichia coli
Na+ activates, can partially replace Mg2+ Escherichia coli
NH4+ activates, can partially replace Mg2+ Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 high speed equilibrium sedimentation Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli B / ATCC 11303
-
-
-

Purification (Commentary)

Purification (Comment) Organism
over 1500fold purification Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14
-
-
 Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, 1 month, 30% loss of activity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O formylates methionyl-tRNAfMet from E. coli Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O formylates methionyl-tRNAfMet from E. coli Escherichia coli B / ATCC 11303 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
10-formyltetrahydrofolate + L-methionyl-tRNAMet
-
 Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAMet
-
 r
10-formyltetrahydrofolate + L-methionyl-tRNAMet
-
 Escherichia coli B / ATCC 11303 tetrahydrofolate + N-formylmethionyl-tRNAMet
-
 r
additional information no formylation of deacylated heterogenous or deacylated partially purified methionyl-tRNA Escherichia coli ?
-
 ?
additional information no formyl group donor: 5,10-methenyltetrahydrofolate Escherichia coli ?
-
 ?
additional information no formylation of deacylated heterogenous or deacylated partially purified methionyl-tRNA Escherichia coli B / ATCC 11303 ?
-
 ?
additional information no formyl group donor: 5,10-methenyltetrahydrofolate Escherichia coli B / ATCC 11303 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 with abrupt decrease of reaction rate below pH 7.0 Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
6.8 8.4 about 60% of maximal activity at pH 6.8 and 8.4 Escherichia coli
7
-
 below: rapid decrease in reaction rate Escherichia coli

Cofactor

Cofactor Comment Organism Structure
10-formyltetrahydrofolate
-
 Escherichia coli
additional information no cofactors: ribonucleotide mono- or triphosphates Escherichia coli
additional information no cofactors: ATP, GTP Escherichia coli
tetrahydrofolate
-
 Escherichia coli