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Literature summary for 2.1.2.8 extracted from
- On the inhibition of deoxycytidylate hydroxymethylase by 5-fluoro-2-deoxycytidine 5-monophosphate (1989), Arch. Biochem. Biophys., 275, 11-15.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-Fluoro-2'-deoxycytidine 5'-monophosphate | competitive inhibitor with respect to dCMP, not mechanism-based inhibitor | Tequatrovirus T4 |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.36 | - |
deoxycytidylate | - |
Tequatrovirus T4 | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate | Tequatrovirus T4 | - |
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | enzyme is responsible for production of 5-hydroxymethyl-dCMP found in the DNA of the phage | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tequatrovirus T4 | - |
Escherichia coli infected with bacteriophage T4 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Tequatrovirus T4 |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate = tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | mechanism | Tequatrovirus T4 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1 | - |
- |
Tequatrovirus T4 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate | - |
Tequatrovirus T4 | tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | - |
? | |
| 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate | - |
Tequatrovirus T4 | tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | enzyme is responsible for production of 5-hydroxymethyl-dCMP found in the DNA of the phage | ? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Tequatrovirus T4 |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 5,10-methylenetetrahydrofolate | as cofactor | Tequatrovirus T4 | |
| tetrahydrofolate | - |
Tequatrovirus T4 | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.025 | - |
5-Fluoro-2'-deoxycytidine 5'-monophosphate | - |
Tequatrovirus T4 | |
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