Crystallization (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
General Stability | Organism |
---|---|
tetrahydrofolate plus pyridoxal 5-phosphate plus hydroxymethylserine increase temperature-stability | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | at higher concentrations | Pseudomonas sp. | |
DL-cycloserine | strong, competitive | Pseudomonas sp. | |
Fe2+ | at higher concentrations | Pseudomonas sp. | |
Fe3+ | at higher concentrations | Pseudomonas sp. | |
L-Cycloserine | - |
Pseudomonas sp. | |
L-serine | weak, competitive | Pseudomonas sp. | |
additional information | iodoacetate, GSH; no inhibition by Mg2+, Mn2+, Al3+, 2-mercaptoethanol | Pseudomonas sp. | |
p-chloromercuribenzoate | - |
Pseudomonas sp. | |
Zn2+ | at higher concentrations | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
L-tetrahydrofolate | pH 7.5, calculated on the basis of one isomer | Pseudomonas sp. | |
0.8 | - |
hydroxymethylserine | pH 7.5 | Pseudomonas sp. | |
1.5 | - |
2-methyl-DL-serine | pH 7.5 | Pseudomonas sp. | |
6.2 | - |
2-ethyl-DL-serine | pH 7.5 | Pseudomonas sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Pseudomonas sp. | 5737 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
inducible enzyme | - |
Pseudomonas sp. MS | - |
inducible enzyme | - |
Purification (Comment) | Organism |
---|---|
partial | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
17.4 | - |
- |
Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tetrahydrofolate + (+)-2-methylserine | stereospecific, dimedon cannot replace tetrahydrofolate | Pseudomonas sp. | 5,10-methylenetetrahydrofolate + D-alanine + H2O | 5,10-methylenetetrahydrofolate is non-enzymatically cleaved to tetrahydrofolate and formaldehyde | r | |
tetrahydrofolate + (+)-2-methylserine | stereospecific, dimedon cannot replace tetrahydrofolate | Pseudomonas sp. MS | 5,10-methylenetetrahydrofolate + D-alanine + H2O | 5,10-methylenetetrahydrofolate is non-enzymatically cleaved to tetrahydrofolate and formaldehyde | r | |
tetrahydrofolate + (-)-ethylserine | - |
Pseudomonas sp. | 5,10-methylenetetrahydrofolate + D-2-aminobutyrate + H2O | - |
r | |
tetrahydrofolate + (-)-ethylserine | - |
Pseudomonas sp. MS | 5,10-methylenetetrahydrofolate + D-2-aminobutyrate + H2O | - |
r | |
tetrahydrofolate + 2-ethyl-DL-serine | - |
Pseudomonas sp. | 5,10-methylenetetrahydrofolate + D-2-aminobutyrate + H2O | - |
r | |
tetrahydrofolate + 2-ethyl-DL-serine | - |
Pseudomonas sp. MS | 5,10-methylenetetrahydrofolate + D-2-aminobutyrate + H2O | - |
r | |
tetrahydrofolate + 2-hydroxymethylserine | - |
Pseudomonas sp. | 5,10-methylenetetrahydrofolate + D-serine + H2O | - |
? | |
tetrahydrofolate + 2-hydroxymethylserine | - |
Pseudomonas sp. MS | 5,10-methylenetetrahydrofolate + D-serine + H2O | - |
? | |
tetrahydrofolate + 2-methyl-DL-serine | - |
Pseudomonas sp. | 5,10-methylenetetrahydrofolate + D-alanine + H2O | - |
r | |
tetrahydrofolate + 2-methyl-DL-serine | - |
Pseudomonas sp. MS | 5,10-methylenetetrahydrofolate + D-alanine + H2O | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Pseudomonas sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
33 | 48 | about half-maximal activity at 33°C and 90% activity at 48°C | Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
96% loss of activity after 10 min, hydroxymethylserine plus tetrahydrofolate and pyridoxal 5'-phosphate prevent | Pseudomonas sp. |
50 | - |
complete inactivation after 10 min, 55% loss of activity in the presence of tetrahydrofolate, pyridoxal 5'-phosphate plus hydroxymethylserine or D-serine | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Pseudomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10.5 | about half-maximal activity at pH 6.0 and 10.5 | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | requirement | Pseudomonas sp. | |
pyridoxal 5'-phosphate | Km: 0.01 mM at pH 7.5 | Pseudomonas sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0022 | - |
D-cycloserine | - |
Pseudomonas sp. | |
0.005 | - |
L-Cycloserine | - |
Pseudomonas sp. | |
5.6 | - |
L-serine | - |
Pseudomonas sp. |