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Literature summary for 2.1.2.5 extracted from

  • Miller, A.; Waelsch, H.
    Formimino transfer from formamidinoglutaric acid to tetrahydrofolic acid (1957), J. Biol. Chem., 228, 397-417.
    View publication on PubMed

General Stability

General Stability Organism
no loss of activity after dialysis against phosphate buffer or 0.05 M EDTA, pH 7.4 Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
acetate inhibition in decreasing order: fumarate, succinate, citrate, acetate Bos taurus
acetyl-L-glutamate 0.015 mM, 30% inhibition Bos taurus
arsenate weak Bos taurus
Ca2+ 0.1 M, strong inhibition in decreasing order: La3+, Ca2+, Mg2+, Li+, Na+, K+ Bos taurus
citrate inhibition in decreasing order: fumarate, succinate, citrate, acetate Bos taurus
D-glutamate 0.015 mM, 47% inhibition Bos taurus
folic acid 0.3 mM, 20% inhibition Bos taurus
formyl-L-glutamate 0.015 mM, 53% inhibition Bos taurus
fumarate inhibition in decreasing order: fumarate, succinate, citrate, acetate Bos taurus
K+ 0.1 M, strong inhibition in decreasing order: La3+, Ca2+, Mg2+, Li+, Na+, K+ Bos taurus
K2SO4 0.1 M, 30% inhibition Bos taurus
KCl 0.1 M, 30% inhibition Bos taurus
KNO3 0.1 M, 65% inhibition Bos taurus
L-aspartate 0.015 mM, 26% inhibition Bos taurus
L-glutamate 0.015 mM, 93% inhibition Bos taurus
La3+ 0.1 M, strong inhibition in decreasing order: La3+, Ca2+, Mg2+, Li+, Na+, K+ Bos taurus
Li+ 0.1 M, strong inhibition in decreasing order: La3+, Ca2+, Mg2+, Li+, Na+, K+ Bos taurus
Mg2+ 0.1 M, strong inhibition in decreasing order: La3+, Ca2+, Mg2+, Li+, Na+, K+ Bos taurus
additional information not inhibited by formamidinoacetic acid, gamma-benzyl-formamidinoglutaric acid, formic acid, formamide, formylglutamine, formylisoglutamine Bos taurus
Na+ 0.1 M, strong inhibition in decreasing order: La3+, Ca2+, Mg2+, Li+, Na+, K+ Bos taurus
p-acetylaminobenzoylglutamate 0.3 mM, 50% inhibition Bos taurus
p-aminobenzoylglutamate 2.5 mM, 50% inhibition Bos taurus
p-formylaminobenzoylglutamate 0.3 mM, 50% inhibition Bos taurus
p-iodobenzoylglutamate 1.5 mM, 50% inhibition Bos taurus
phosphate weak Bos taurus
succinate inhibition in decreasing order: fumarate, succinate, citrate, acetate Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Rattus norvegicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
NH4+ 0.1 M, 10% stimulation Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
calf
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
60-80fold purification Bos taurus

Reaction

Reaction Comment Organism Reaction ID
5-formimidoyltetrahydrofolate + L-glutamate = tetrahydrofolate + N-formimidoyl-L-glutamate mechanism Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
kidney low activity Rattus norvegicus
-
kidney low activity Bos taurus
-
liver
-
Rattus norvegicus
-
liver
-
Bos taurus
-
additional information no activity in the soluble proteins of brain, heart, lung, testis, intestinal mucosa, diaphragm muscle or spleen Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Bos taurus

Storage Stability

Storage Stability Organism
-15°C, crude extract, 1 year, stable Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-formyltetrahydrofolate + L-glutamate formation of N5-formyltetrahydrofolate + L-glutamate Bos taurus N-formyl-L-glutamate + tetrahydrofolate
-
?
additional information
-
Rattus norvegicus ?
-
?
additional information primary reaction of the over-all reaction formimino-L-glutamate + tetrahydrofolate = N10-formyltetrahydrofolate + glutamate + NH3 Bos taurus ?
-
?
additional information not: formamidinoacetic acid, formylisoglutamine, formylglutamine, acetylglutamic acid Bos taurus ?
-
?
additional information formation of glutamic acid is reversible while formation of ammonia is not Bos taurus ?
-
?
tetrahydrofolate + N-formimino-L-glutamate
-
Rattus norvegicus 5-formiminotetrahydrofolate + L-glutamate
-
?
tetrahydrofolate + N-formimino-L-glutamate alpha-L-formamidinoglutaric acid, absolute specificity with regard to the isomers of tetrahydrofolate at position 6, reduced pteroylaspartic acid and reduced pteroyltriglutamic acid can replace tetrahydrofolate Bos taurus 5-formiminotetrahydrofolate + L-glutamate
-
r
tetrahydrofolate + N-formimino-L-glutamate specificity, equilibrium lies far on formation of formiminotetrahydrofolate and glutamate Bos taurus 5-formiminotetrahydrofolate + L-glutamate
-
r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 9 unbuffered solution Bos taurus
6.5 7.6 phosphate buffer Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
6 9.8 about 50% of activity maximum at pH 6 and 9.8 Bos taurus

Cofactor

Cofactor Comment Organism Structure
tetrahydrofolate
-
Rattus norvegicus
tetrahydrofolate
-
Bos taurus