General Stability | Organism |
---|---|
no loss of activity after dialysis against phosphate buffer or 0.05 M EDTA, pH 7.4 | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetate | inhibition in decreasing order: fumarate, succinate, citrate, acetate | Bos taurus | |
acetyl-L-glutamate | 0.015 mM, 30% inhibition | Bos taurus | |
arsenate | weak | Bos taurus | |
Ca2+ | 0.1 M, strong inhibition in decreasing order: La3+, Ca2+, Mg2+, Li+, Na+, K+ | Bos taurus | |
citrate | inhibition in decreasing order: fumarate, succinate, citrate, acetate | Bos taurus | |
D-glutamate | 0.015 mM, 47% inhibition | Bos taurus | |
folic acid | 0.3 mM, 20% inhibition | Bos taurus | |
formyl-L-glutamate | 0.015 mM, 53% inhibition | Bos taurus | |
fumarate | inhibition in decreasing order: fumarate, succinate, citrate, acetate | Bos taurus | |
K+ | 0.1 M, strong inhibition in decreasing order: La3+, Ca2+, Mg2+, Li+, Na+, K+ | Bos taurus | |
K2SO4 | 0.1 M, 30% inhibition | Bos taurus | |
KCl | 0.1 M, 30% inhibition | Bos taurus | |
KNO3 | 0.1 M, 65% inhibition | Bos taurus | |
L-aspartate | 0.015 mM, 26% inhibition | Bos taurus | |
L-glutamate | 0.015 mM, 93% inhibition | Bos taurus | |
La3+ | 0.1 M, strong inhibition in decreasing order: La3+, Ca2+, Mg2+, Li+, Na+, K+ | Bos taurus | |
Li+ | 0.1 M, strong inhibition in decreasing order: La3+, Ca2+, Mg2+, Li+, Na+, K+ | Bos taurus | |
Mg2+ | 0.1 M, strong inhibition in decreasing order: La3+, Ca2+, Mg2+, Li+, Na+, K+ | Bos taurus | |
additional information | not inhibited by formamidinoacetic acid, gamma-benzyl-formamidinoglutaric acid, formic acid, formamide, formylglutamine, formylisoglutamine | Bos taurus | |
Na+ | 0.1 M, strong inhibition in decreasing order: La3+, Ca2+, Mg2+, Li+, Na+, K+ | Bos taurus | |
p-acetylaminobenzoylglutamate | 0.3 mM, 50% inhibition | Bos taurus | |
p-aminobenzoylglutamate | 2.5 mM, 50% inhibition | Bos taurus | |
p-formylaminobenzoylglutamate | 0.3 mM, 50% inhibition | Bos taurus | |
p-iodobenzoylglutamate | 1.5 mM, 50% inhibition | Bos taurus | |
phosphate | weak | Bos taurus | |
succinate | inhibition in decreasing order: fumarate, succinate, citrate, acetate | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Rattus norvegicus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NH4+ | 0.1 M, 10% stimulation | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
calf | - |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
60-80fold purification | Bos taurus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
5-formimidoyltetrahydrofolate + L-glutamate = tetrahydrofolate + N-formimidoyl-L-glutamate | mechanism | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | low activity | Rattus norvegicus | - |
kidney | low activity | Bos taurus | - |
liver | - |
Rattus norvegicus | - |
liver | - |
Bos taurus | - |
additional information | no activity in the soluble proteins of brain, heart, lung, testis, intestinal mucosa, diaphragm muscle or spleen | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bos taurus |
Storage Stability | Organism |
---|---|
-15°C, crude extract, 1 year, stable | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-formyltetrahydrofolate + L-glutamate | formation of N5-formyltetrahydrofolate + L-glutamate | Bos taurus | N-formyl-L-glutamate + tetrahydrofolate | - |
? | |
additional information | - |
Rattus norvegicus | ? | - |
? | |
additional information | primary reaction of the over-all reaction formimino-L-glutamate + tetrahydrofolate = N10-formyltetrahydrofolate + glutamate + NH3 | Bos taurus | ? | - |
? | |
additional information | not: formamidinoacetic acid, formylisoglutamine, formylglutamine, acetylglutamic acid | Bos taurus | ? | - |
? | |
additional information | formation of glutamic acid is reversible while formation of ammonia is not | Bos taurus | ? | - |
? | |
tetrahydrofolate + N-formimino-L-glutamate | - |
Rattus norvegicus | 5-formiminotetrahydrofolate + L-glutamate | - |
? | |
tetrahydrofolate + N-formimino-L-glutamate | alpha-L-formamidinoglutaric acid, absolute specificity with regard to the isomers of tetrahydrofolate at position 6, reduced pteroylaspartic acid and reduced pteroyltriglutamic acid can replace tetrahydrofolate | Bos taurus | 5-formiminotetrahydrofolate + L-glutamate | - |
r | |
tetrahydrofolate + N-formimino-L-glutamate | specificity, equilibrium lies far on formation of formiminotetrahydrofolate and glutamate | Bos taurus | 5-formiminotetrahydrofolate + L-glutamate | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | unbuffered solution | Bos taurus |
6.5 | 7.6 | phosphate buffer | Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9.8 | about 50% of activity maximum at pH 6 and 9.8 | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
tetrahydrofolate | - |
Rattus norvegicus | |
tetrahydrofolate | - |
Bos taurus |