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Literature summary for 2.1.2.3 extracted from

  • Axelrod, H.L.; McMullan, D.; Krishna, S.S.; Miller, M.D.; Elsliger, M.; Abdubek, P.; Ambing, E.; Astakhova, T.; Carlton, D.; Chiu, H.; Clayton, T.; Duan, L.; Feuerhelm, J.; Grzechnik, S.K.; Hale, J.; Han, G.W.; Haugen, J.; Jaroszewski, L.; Jin, K.K.; Klock, H.E.; Knuth, M.W.; Koesema, E.; Morse, A.T.; Nigoghossian, E.; Okach, L.; Oommachen, S.; Paulsen, J.; Quijano, K.; Reyes, R.; Rife, C.L.; van den Bedem, H.; Weekes, D.; White, A.; Wolf, G.; Xu, Q.; Hodgson, K.O.; Wooley, J.; Deacon. A.M.; Godzik, A.; Lesley, S.A.; Wilson, I.A.
    Crystal structure of AICAR transformylase IMP cyclohydrolase (TM1249) from Thermotoga maritima at 1.88 ANG. resolution (2008), Proteins Struct. Funct. Bioinform., 71, 1042-1049.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
into the plasmid pMH4 for expression in the Escherichia coli strain GeneHogs Thermotoga maritima

Crystallization (Commentary)

Crystallization (Comment) Organism
the three-dimensional structure of TM1249 is determined to 1.88 A resolution by molecular replacement Thermotoga maritima

Metals/Ions

Metals/Ions Comment Organism Structure
K+ the refined structure includes two K+ ions in the asymmetric unit Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49700
-
monomer, residues 1-452 Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide Thermotoga maritima
-
tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
?

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using a nickel-chelating resin and a Resource Q column Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
Thermotoga maritima tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
?

Subunits

Subunits Comment Organism
dimer
-
Thermotoga maritima

Synonyms

Synonyms Comment Organism
5-aminoimidazole-4-carboxamide ribonucleotide transformylase
-
Thermotoga maritima
AICAR TFase
-
Thermotoga maritima
AICAR transformylase
-
Thermotoga maritima
PurH
-
Thermotoga maritima
TM1249
-
Thermotoga maritima

Cofactor

Cofactor Comment Organism Structure
10-formyltetrahydrofolate
-
Thermotoga maritima

pI Value

Organism Comment pI Value Maximum pI Value
Thermotoga maritima calculated isoelectric point
-
5.49