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Literature summary for 2.1.2.2 extracted from

  • Manieri, W.; Moore, M.E.; Soellner, M.B.; Tsang, P.; Caperelli, C.A.
    Human glycinamide ribonucleotide transformylase: active site mutants as mechanistic probes (2007), Biochemistry, 46, 156-163.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.2.2

Application

Application Comment Organism
medicine glycinamide ribonucleotide transformylase is a validated target for cancer chemotherapy Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
into the pET17b vector and into the pMC8HdeltaT vector for expression in Escherichia coli BL21Star(DE3)pRARE cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
D144E catalytically inactive mutant Homo sapiens
D144H catalytically inactive mutant Homo sapiens
H108K catalytically inactive mutant Homo sapiens
H108R catalytically inactive mutant Homo sapiens
K170R catalytically active mutant Homo sapiens
N106D catalytically inactive mutant Homo sapiens
N106H catalytically inactive mutant Homo sapiens
N106Q catalytically active mutant Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0009
-
 10-formyl-5,8-dideazafolate wild type enzyme Homo sapiens
0.0011
-
 beta-glycinamide ribonucleotide wild type enzyme Homo sapiens
0.0015
-
 10-formyl-5,8-dideazafolate mutant K170R Homo sapiens
0.0018
-
 beta-glycinamide ribonucleotide mutant K170R Homo sapiens
0.0196
-
 10-formyl-5,8-dideazafolate mutant N106Q Homo sapiens
0.0295
-
 beta-glycinamide ribonucleotide mutant N106Q Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
108000
-
 human GART comprises the C-terminal domain of a 108 kDa, trifunctional enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using a NiHisTrap HP column, the affinity tag is removed by cleavage with TEV protease Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-formyl-5,8-dideazafolate + N1-(5-phospho-D-ribosyl)glycinamide
-
 Homo sapiens ?
-
 ?
10-formyltetrahydrofolate + N1-(5-phospho-D-ribosyl)glycinamide
-
 Homo sapiens tetrahydrofolate + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide
-
 ?
beta-glycinamide ribonucleotide + 10-formyltetrahydrofolate
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
GART
-
 Homo sapiens
glycinamide ribonucleotide transformylase
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.5
-
 beta-glycinamide ribonucleotide wild type enzyme Homo sapiens
7.7
-
 beta-glycinamide ribonucleotide mutant K170R Homo sapiens
14
-
 beta-glycinamide ribonucleotide mutant N106Q Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
5 11
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
folate
-
 Homo sapiens