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Literature summary for 2.1.2.2 extracted from

  • Caperelli, C.A.; Giroux, E.L.
    The human glycinamide ribonucleotide transformylase domain: purification, characterization, and kinetic mechanism (1997), Arch. Biochem. Biophys., 341, 98-103.
    View publication on PubMed

Application

Application Comment Organism
medicine pharmacological importance, target enzyme for chemotherapy, development of novel antifolate drugs, to be used as anti-cancer drugs Escherichia coli
medicine pharmacological importance, target enzyme for chemotherapy, development of novel antifolate drugs, to be used as anti-cancer drugs Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
cloned and expressed in Escherichia coli BL21(DE3)pLysS Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
(alpha,beta)-hydroxyacetamide ribonucleotide
-
Homo sapiens
10-Acetyl-5,8-dideazafolate
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0011
-
beta-glycinamide ribonucleotide
-
Homo sapiens
0.0015
-
10-formyl-5,8-dideazafolate recombinant enzyme, expressed in E.coli Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21800
-
recombinant enzyme, predicted from cDNA Homo sapiens
21840
-
recombinant enzyme, MALDI-TOF mass spectral analysis Homo sapiens
25700
-
SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Bacillus subtilis
-
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Saccharomyces cerevisiae
-
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Gallus gallus essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Escherichia coli essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Homo sapiens essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Escherichia coli
-
-
-
Gallus gallus
-
chicken
-
Homo sapiens
-
human
-
Saccharomyces cerevisiae
-
yeast
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, expressed in E. coli Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Gallus gallus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
Gallus gallus tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
Bacillus subtilis tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
Escherichia coli tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
Homo sapiens tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
Saccharomyces cerevisiae tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway Gallus gallus tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway Escherichia coli tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway Homo sapiens tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
?
additional information monofunctional enzyme Bacillus subtilis ?
-
?
additional information monofunctional enzyme Escherichia coli ?
-
?
additional information trifunctional enzyme, GAR synthetase, aminoimidazole ribonucleotide synthetase and GAR transformylase Gallus gallus ?
-
?
additional information trifunctional enzyme, GAR synthetase, aminoimidazole ribonucleotide synthetase and GAR transformylase Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
monomer
-
Gallus gallus
monomer
-
Bacillus subtilis
monomer
-
Escherichia coli
monomer
-
Saccharomyces cerevisiae
monomer 1 * 25700, SDS-PAGE Homo sapiens