Application | Comment | Organism |
---|---|---|
medicine | pharmacological importance, target enzyme for chemotherapy, development of novel antifolate drugs, to be used as anti-cancer drugs | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00025 | - |
10-formyl-5,8-dideazafolate | - |
Mus musculus | |
0.0008 | - |
L-(-)-10-formyltetrahydrofolate | - |
Mus musculus | |
0.0019 | - |
10-formyl-5,8-dideazafolate | - |
Gallus gallus | |
0.4 | - |
glycinamide ribonucleotide | - |
Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61000 | - |
2 * 61000, SDS-PAGE | Gallus gallus |
102000 | - |
1 * 102000, SDS-PAGE | Mus musculus |
110000 | - |
1 * 110000, SDS-PAGE | Mus musculus |
125000 | - |
sucrose density gradient ultracentrifugation | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | Mus musculus | in ureotelic animals such as mammals the role of the far less active pathway is to provide purine nucleotides for further anabolic sequences | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | Gallus gallus | in uricotelic organisms such as birds, the major role of this active pathway is elimination of waste nitrogen | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | Gallus gallus | essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | Mus musculus | essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
chicken | - |
Mus musculus | - |
mouse | - |
Purification (Comment) | Organism |
---|---|
- |
Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
L-5178-Y cell | murine lymphoma cell line | Mus musculus | - |
liver | - |
Gallus gallus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.3 | - |
- |
Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyl-5,8-dideazafolate + carbocyclic beta-glycinamide ribonucleotide | - |
Mus musculus | 5,8-dideazafolate + N-formyl carbocyclic beta-glycinamide ribonucleotide | - |
? | |
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | - |
Gallus gallus | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | - |
Mus musculus | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | in ureotelic animals such as mammals the role of the far less active pathway is to provide purine nucleotides for further anabolic sequences | Mus musculus | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | in uricotelic organisms such as birds, the major role of this active pathway is elimination of waste nitrogen | Gallus gallus | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway | Gallus gallus | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway | Mus musculus | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
5'-phosphoribosylglycinamide + 10-formyl-5,8-dideazafolate | - |
Gallus gallus | 5'-phosphoribosyl-N-formylglycinamide + 5,8-dideazafolate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 61000, SDS-PAGE | Gallus gallus |
monomer | - |
Gallus gallus |
monomer | 1 * 110000, SDS-PAGE | Mus musculus |
monomer | 1 * 102000, SDS-PAGE | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
10-formyl-5,8-dideazafolate | - |
Mus musculus |