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Literature summary for 2.1.2.2 extracted from
- Purification of a complex catalizing folate cofactor synthesis and transformylation in de novo purine biosynthesis (1980), J. Biol. Chem., 255, 1885-1890.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 61000 | - |
2 * 61000, GAR transformylase, SDS-PAGE | Gallus gallus |
| 95000 | - |
2 * 95000, trifunctional protein, SDS-PAGE | Gallus gallus |
| 110000 | - |
GAR transformylase, sucrose density gradient ultracentrifugation | Gallus gallus |
| 150000 | - |
- |
Saccharomyces cerevisiae |
| 190000 | - |
trifunctional protein, sucrose density gradient ultracentrifugation | Gallus gallus |
| 201000 | - |
- |
Ovis aries |
| 215000 | - |
- |
Oryctolagus cuniculus |
| 218000 | - |
- |
Sus scrofa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | Sus scrofa | - |
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? |  |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | Saccharomyces cerevisiae | - |
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | Ovis aries | - |
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | Columba sp. | - |
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | Gallus gallus | essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Columba sp. | - |
pigeon | - |
| Gallus gallus | - |
chicken | - |
| Oryctolagus cuniculus | - |
rabbit | - |
| Ovis aries | - |
sheep | - |
| Saccharomyces cerevisiae | - |
yeast | - |
| Sus scrofa | - |
pig | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Gallus gallus |
- |
Sus scrofa |
- |
Saccharomyces cerevisiae |
- |
Oryctolagus cuniculus |
- |
Ovis aries |
- |
Columba sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Gallus gallus | - |
| liver | - |
Sus scrofa | - |
| liver | - |
Oryctolagus cuniculus | - |
| liver | - |
Ovis aries | - |
| liver | - |
Columba sp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.651 | - |
- |
Gallus gallus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | - |
Gallus gallus | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | - |
Sus scrofa | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | - |
Saccharomyces cerevisiae | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | - |
Ovis aries | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | - |
Columba sp. | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway | Gallus gallus | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| additional information | purified along with 10-formyltetrahydrofolate synthetase | Gallus gallus | ? | - |
? | |
| additional information | uses both diastereomers of 5,10-methenyltetrahydrofolate, (+)-L-diastereomer is used 120 times faster than the (-)-L-diastereomer | Gallus gallus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Saccharomyces cerevisiae |
| dimer | - |
Ovis aries |
| dimer | 2 * 61000, GAR transformylase, SDS-PAGE | Gallus gallus |
| dimer | 2 * 95000, trifunctional protein, SDS-PAGE | Gallus gallus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 5,10-methenyltetrahydrofolate | - |
Gallus gallus | |
| N10-formyltetrahydrofolate | - |
Gallus gallus | |
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