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Literature summary for 2.1.2.1 extracted from

  • Kumar, A.; Wu, G.; Wu, Z.; Kumar, N.; Liu, Z.
    Improved catalytic properties of a serine hydroxymethyl transferase from Idiomarina loihiensis by site directed mutagenesis (2018), Int. J. Biol. Macromol., 117, 1216-1223 .
    View publication on PubMed
Search for more literature for 2.1.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Idiomarina loihiensis

Protein Variants

Protein Variants Comment Organism
G132P the mutant shows 301% activity compared to the wild type enzyme Idiomarina loihiensis
H61G the mutant shows 283% activity compared to the wild type enzyme Idiomarina loihiensis
H61G/G132P the mutant shows 356% activity compared to the wild type enzyme Idiomarina loihiensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
 DL-phenylserine wild type enzyme, at pH 7.0 and 37°C Idiomarina loihiensis
81.9
-
 DL-phenylserine mutant enzyme G132P, at pH 7.0 and 37°C Idiomarina loihiensis
93.2
-
 DL-phenylserine mutant enzyme H61G/G132P, at pH 7.0 and 37°C Idiomarina loihiensis
131.6
-
 DL-phenylserine mutant enzyme H61G, at pH 7.0 and 37°C Idiomarina loihiensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + glycine + H2O Idiomarina loihiensis
-
 tetrahydrofolate + L-serine
-
 r
tetrahydrofolate + L-serine Idiomarina loihiensis
-
 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r

Organism

Organism UniProt Comment Textmining
Idiomarina loihiensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione-Sepharose resin column chromatography and Superdex S200 gel filtration Idiomarina loihiensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + glycine + H2O
-
 Idiomarina loihiensis tetrahydrofolate + L-serine
-
 r
DL-phenylserine + ?
-
 Idiomarina loihiensis benzaldehyde + ?
-
 ?
tetrahydrofolate + L-serine
-
 Idiomarina loihiensis 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r

Subunits

Subunits Comment Organism
? x * 45400, SDS-PAGE and calculated from amino acid sequence Idiomarina loihiensis

Synonyms

Synonyms Comment Organism
GlyA
-
 Idiomarina loihiensis
serine hydroxymethyl transferase
-
 Idiomarina loihiensis
SHMT
-
 Idiomarina loihiensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Idiomarina loihiensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 60 more than 45% activity between 35 and 60°C Idiomarina loihiensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45 65 the wild type enzyme shows a rapid decrease (to less than 20%) in activity after 30 min at 55-65°C, and shows about 35% activity after 30 min at 45°C Idiomarina loihiensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.51
-
 DL-phenylserine wild type enzyme, at pH 7.0 and 37°C Idiomarina loihiensis
2.34
-
 DL-phenylserine mutant enzyme H61G, at pH 7.0 and 37°C Idiomarina loihiensis
3.67
-
 DL-phenylserine mutant enzyme G132P, at pH 7.0 and 37°C Idiomarina loihiensis
6.89
-
 DL-phenylserine mutant enzyme H61G/G132P, at pH 7.0 and 37°C Idiomarina loihiensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Idiomarina loihiensis

pH Range

pH Minimum pH Maximum Comment Organism
7 10 about 20% activity at pH 6.5, about 85% activity at pH 7.0, about 95% activity at pH 8.0, about 75% activity at pH 9.0, about 45% activity at pH 10.0 Idiomarina loihiensis

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Idiomarina loihiensis