Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bombyx mori |
Protein Variants | Comment | Organism |
---|---|---|
H119A | the mutant shows reduced activity compared to the wild type enzyme | Bombyx mori |
H132A | the mutant shows reduced activity compared to the wild type enzyme | Bombyx mori |
H135A | the mutant shows reduced activity compared to the wild type enzyme | Bombyx mori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
tetrahydrofolate | mutant enzyme H135A, in the presence of NADP+, at pH 3.0 and 30°C | Bombyx mori | |
0.0031 | - |
tetrahydrofolate | mutant enzyme H119A, in the presence of NADP+, at pH 3.0 and 30°C | Bombyx mori | |
0.014 | - |
tetrahydrofolate | mutant enzyme H132A, in the presence of NADP+, at pH 3.0 and 30°C | Bombyx mori | |
0.055 | - |
tetrahydrofolate | wild type enzyme, in the presence of NADP+, at pH 3.0 and 30°C | Bombyx mori | |
1.8 | - |
L-serine | wild type enzyme, in the presence of NADP+, at pH 3.0 and 30°C | Bombyx mori | |
3.3 | - |
L-serine | mutant enzyme H135A, in the presence of NADP+, at pH 3.0 and 30°C | Bombyx mori | |
4.2 | - |
L-serine | mutant enzyme H119A, in the presence of NADP+, at pH 3.0 and 30°C | Bombyx mori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + glycine + H2O | Bombyx mori | - |
tetrahydrofolate + L-serine | - |
r | |
5,10-methylenetetrahydrofolate + glycine + H2O | Bombyx mori p50T | - |
tetrahydrofolate + L-serine | - |
r | |
tetrahydrofolate + L-serine | Bombyx mori | - |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
r | |
tetrahydrofolate + L-serine | Bombyx mori p50T | - |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bombyx mori | - |
- |
- |
Bombyx mori p50T | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-affinity column chromatography and Superdex S200 gel filtration | Bombyx mori |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fat body | - |
Bombyx mori | - |
hemocyte | - |
Bombyx mori | - |
larva | - |
Bombyx mori | - |
midgut | - |
Bombyx mori | - |
ovary | highest expression | Bombyx mori | - |
silk gland | - |
Bombyx mori | - |
testis | high expression | Bombyx mori | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + glycine + H2O | - |
Bombyx mori | tetrahydrofolate + L-serine | - |
r | |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
Bombyx mori p50T | tetrahydrofolate + L-serine | - |
r | |
tetrahydrofolate + L-serine | - |
Bombyx mori | 5,10-methylenetetrahydrofolate + glycine + H2O | - |
r | |
tetrahydrofolate + L-serine | - |
Bombyx mori p50T | 5,10-methylenetetrahydrofolate + glycine + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE | Bombyx mori |
Synonyms | Comment | Organism |
---|---|---|
serine hydroxymethyltransferase | - |
Bombyx mori |
SHMT | - |
Bombyx mori |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Bombyx mori |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 35 | the enzyme shows about 35% activity at 25 or 35°C | Bombyx mori |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 40 | the enzyme retains more than 80% of its activity after 30 min at 20-40°C. After 30 min at 45-50°C, the enzyme shows about 30% residual activity. The enzyme shows about 30% activity after 30 min at pH 10-12 | Bombyx mori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
- |
Bombyx mori |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 4 | about 60% activity at pH 2.0, 100% activity at pH 3.0, about 20% activity at pH 4.0 | Bombyx mori |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | 7 | the enzyme retains more than 50% of its activity after 30 min at pH 2.0-7.0 | Bombyx mori |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Bombyx mori |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00065 | - |
L-serine | mutant enzyme H119A, in the presence of NADP+, at pH 3.0 and 30°C | Bombyx mori | |
0.00071 | - |
L-serine | mutant enzyme H135A, in the presence of NADP+, at pH 3.0 and 30°C | Bombyx mori | |
0.0022 | - |
L-serine | wild type enzyme, in the presence of NADP+, at pH 3.0 and 30°C | Bombyx mori | |
0.011 | - |
tetrahydrofolate | mutant enzyme H132A, in the presence of NADP+, at pH 3.0 and 30°C | Bombyx mori | |
0.047 | - |
tetrahydrofolate | mutant enzyme H135A, in the presence of NADP+, at pH 3.0 and 30°C | Bombyx mori | |
0.074 | - |
tetrahydrofolate | mutant enzyme H119A, in the presence of NADP+, at pH 3.0 and 30°C | Bombyx mori | |
0.081 | - |
tetrahydrofolate | wild type enzyme, in the presence of NADP+, at pH 3.0 and 30°C | Bombyx mori |