Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.2.1 extracted from

  • Yuan, S.; Jiang, W.; Chen, L.; Guo, Y.; Liu, Z.
    A novel serine hydroxymethyltransferase from marine bacterium Alcanivorax sp. and its application on enzymatic synthesis of L-serine (2014), J. Mol. Catal. B, 109, 17-23.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
gene glyA, DNa and amino acid sequence determination and analysis, subcloning in Escherichia coli strain DH5alpha, sequence comparison, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21 (DE3) Alcanivorax sp.

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ strong inhibition Alcanivorax sp.
SDS strong inhibition Alcanivorax sp.

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Mg2+, K+, Na+and EDTA have no significant effect on the enzyme activity Alcanivorax sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45200
-
x * 45200, about, sequence calculation, recombinant enzyme, SDS-PAGE Alcanivorax sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + glycine + H2O Alcanivorax sp.
-
tetrahydrofolate + L-serine
-
r

Organism

Organism UniProt Comment Textmining
Alcanivorax sp.
-
gene glyA
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + glycine + H2O
-
Alcanivorax sp. tetrahydrofolate + L-serine
-
r
additional information the enzyme is also active with DL-threo-3-phenylserine Alcanivorax sp. ?
-
?

Subunits

Subunits Comment Organism
? x * 45200, about, sequence calculation, recombinant enzyme, SDS-PAGE Alcanivorax sp.

Synonyms

Synonyms Comment Organism
serine hydroxymethyltransferase
-
Alcanivorax sp.
SHMT
-
Alcanivorax sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Alcanivorax sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 activity range, profile overview Alcanivorax sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Alcanivorax sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9.5 activity range, profile overview Alcanivorax sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 purified recombinant enzyme, over 60% maximal activity remains after 24 h at pH 6.0-7.5, 4°C, less than 20% of maximal activity at pH 8.0 Alcanivorax sp.

Cofactor

Cofactor Comment Organism Structure
5,10-methylenetetrahydrofolate
-
Alcanivorax sp.
pyridoxal 5'-phosphate dependent on Alcanivorax sp.
tetrahydrofolate
-
Alcanivorax sp.