Cloned (Comment) | Organism |
---|---|
gene glyA, DNa and amino acid sequence determination and analysis, subcloning in Escherichia coli strain DH5alpha, sequence comparison, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21 (DE3) | Alcanivorax sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | strong inhibition | Alcanivorax sp. | |
SDS | strong inhibition | Alcanivorax sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Mg2+, K+, Na+and EDTA have no significant effect on the enzyme activity | Alcanivorax sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45200 | - |
x * 45200, about, sequence calculation, recombinant enzyme, SDS-PAGE | Alcanivorax sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + glycine + H2O | Alcanivorax sp. | - |
tetrahydrofolate + L-serine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alcanivorax sp. | - |
gene glyA | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + glycine + H2O | - |
Alcanivorax sp. | tetrahydrofolate + L-serine | - |
r | |
additional information | the enzyme is also active with DL-threo-3-phenylserine | Alcanivorax sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45200, about, sequence calculation, recombinant enzyme, SDS-PAGE | Alcanivorax sp. |
Synonyms | Comment | Organism |
---|---|---|
serine hydroxymethyltransferase | - |
Alcanivorax sp. |
SHMT | - |
Alcanivorax sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Alcanivorax sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | activity range, profile overview | Alcanivorax sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Alcanivorax sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.5 | activity range, profile overview | Alcanivorax sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | purified recombinant enzyme, over 60% maximal activity remains after 24 h at pH 6.0-7.5, 4°C, less than 20% of maximal activity at pH 8.0 | Alcanivorax sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5,10-methylenetetrahydrofolate | - |
Alcanivorax sp. | |
pyridoxal 5'-phosphate | dependent on | Alcanivorax sp. | |
tetrahydrofolate | - |
Alcanivorax sp. |