Cloned (Comment) | Organism |
---|---|
genes pfshmt and PF14_ 0534, encoding isozymes PfSHMTc and PfSHMTm, respectively, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged full-length isozymes | Plasmodium falciparum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
apicoplast | isozyme PfSHMTc in a stage-specific manner, isozyme PfSHMTc localization in this organelle is less restricted than for the mitochondrion and persists from the late trophozoite to the post-mitotic stages | Plasmodium falciparum | 20011 | - |
cytoplasm | isozyme PfSHMTc during all stages | Plasmodium falciparum | 5737 | - |
mitochondrion | isozyme PfSHMTc in a stage-specific manner. Isozyme PfSHMTm showa a distinctly more pronounced mitochondrial location through most of the erythrocytic cycle, presence of a mitochondrial signal sequence | Plasmodium falciparum | 5739 | - |
additional information | patterns of localization of the two isoforms during the parasite erythrocytic cycle, overview. Both PfSHMTc and PfSHMTm are concentrated in the central region of the parasite that becomes the residual body on erythrocyte lysis and merozoite release | Plasmodium falciparum | - |
- |
nucleus | isozyme PfSHMTc during all stages | Plasmodium falciparum | 5634 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49800 | - |
x * 49800, recombinant His-tagged PfSHMTc, SDS-PAGE | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + glycine + H2O | Plasmodium falciparum | - |
tetrahydrofolate + L-serine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
one enzymatically functional SHMT isozyme, PfSHMTc, and one related, apparently inactive isoform, PfSHMTm, encoded by different genes pfshmt and PF14_ 0534, respectively | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
merozoite | both PfSHMTc and PfSHMTm are concentrated in the central region of the parasite that becomes the residual body on erythrocyte lysis and merozoite release | Plasmodium falciparum | - |
trophozoite | early trophozoites lack visible organellar isozyme PfSHMTc, while it occurs during the mid-to-late trophozoite and schizont stages | Plasmodium falciparum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + glycine + H2O | - |
Plasmodium falciparum | tetrahydrofolate + L-serine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 49800, recombinant His-tagged PfSHMTc, SDS-PAGE | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
serine hydroxymethyltransferase | - |
Plasmodium falciparum |
SHMT | - |
Plasmodium falciparum |
General Information | Comment | Organism |
---|---|---|
additional information | both PfSHMTc and PfSHMTm show dynamic, stage-dependent localization among the different compartments of the parasite and sequence analysis suggests they may also reversibly associate with each other, a factor that may be critical to folate cofactor function, given the apparent lack of enzymic activity of PfSHMTm | Plasmodium falciparum |
physiological function | the enzyme is essential for the acquisition of one-carbon units for subsequent transfer reactions | Plasmodium falciparum |