Cloned (Comment) | Organism |
---|---|
expression of wild-type and chimeric mutant in Escherichia coli strain BL21 (DE3) | Bacillus subtilis |
expression of wild-type and chimeric mutant in Escherichia coli strain BL21 (DE3) | Geobacillus stearothermophilus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a chimera from Bacillus stearothermophilus and Bacillus subtilis SHMTs by domain swapping, quarternary structure analysis, overview | Bacillus subtilis |
additional information | generation of a chimera from Bacillus stearothermophilus and Bacillus subtilis SHMTs by domain swapping, quarternary structure analysis, overview | Geobacillus stearothermophilus |
General Stability | Organism |
---|---|
bsSHMT has six unconserved lysine residues in C-terminal domain which render it more resistant to alkaline denaturation. Chemical modification of lysine side chains results in stabilization of monomers | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Geobacillus stearothermophilus | - |
- |
- |
Subunits | Comment | Organism |
---|---|---|
dimer | homology modeling and comparison to SHMT from Bacillus stearothermophilus, using the crystal structure, PDB ID 1KKJ, overview | Bacillus subtilis |
dimer | homology modeling using the crystal structure, PDB ID 1KKJ, and comparison to SHMT from Bacillus subtilis, overview | Geobacillus stearothermophilus |
Synonyms | Comment | Organism |
---|---|---|
serine hydroxymethyltransferase | - |
Bacillus subtilis |
serine hydroxymethyltransferase | - |
Geobacillus stearothermophilus |
SHMT | - |
Bacillus subtilis |
SHMT | - |
Geobacillus stearothermophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
recombinant wild-type bsSHMT and chimeric mutant enzyme are significantly higher susceptible to proteolysis into smaller protein fragments at pH 11 as compared to neutral pH | Bacillus subtilis |
additional information | - |
recombinant wild-type bstSHMT and chimeric mutant enzyme are only merginally susceptible to proteolysis into smaller protein fragments at pH 11 and at neutral pH | Geobacillus stearothermophilus |