Cloned (Comment) | Organism |
---|---|
phylogenetic analysis, expression in Escherichia coli strain Rosetta | Hordeum vulgare |
phylogenetic analysis, expression in Escherichia coli strain Rosetta, transient expression of EGFP-tagged full-length AtSHMT3 in Arabidopsis thaliana leaf protoplasts | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | model of uncompetitive substrate inhibition using tetrahydropteroylglutamates with different numbers of glutamate residues, overview | Arabidopsis thaliana | |
tetrahydropteroylglutamate | - |
Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2177 | - |
tetrahydrofolate | pH 8.5, temperature not specified in the publication, recombinant AtSHMT3 | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Hordeum vulgare | 9507 | - |
chloroplast | - |
Pisum sativum | 9507 | - |
chloroplast | AtSHMT3 | Arabidopsis thaliana | 9507 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + glycine + H2O | Hordeum vulgare | - |
tetrahydrofolate + L-serine | - |
r | |
5,10-methylenetetrahydrofolate + glycine + H2O | Pisum sativum | - |
tetrahydrofolate + L-serine | - |
r | |
5,10-methylenetetrahydrofolate + glycine + H2O | Arabidopsis thaliana | - |
tetrahydrofolate + L-serine | - |
r | |
5,10-methylenetetrahydrofolate + glycine + H2O | Hordeum vulgare Bob | - |
tetrahydrofolate + L-serine | - |
r | |
5,10-methylenetetrahydrofolate + glycine + H2O | Pisum sativum Progress 9 | - |
tetrahydrofolate + L-serine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q94JQ3 | ecotype Columbia | - |
Hordeum vulgare | - |
- |
- |
Hordeum vulgare Bob | - |
- |
- |
Pisum sativum | - |
- |
- |
Pisum sativum Progress 9 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial by chloroplast preparation | Hordeum vulgare |
partial by chloroplast preparation | Pisum sativum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Arabidopsis thaliana | - |
leaf | - |
Hordeum vulgare | - |
leaf | - |
Pisum sativum | - |
rosette leaf | - |
Arabidopsis thaliana | - |
seed | germinated, high expression level of AtSHMT3 | Arabidopsis thaliana | - |
seedling | - |
Arabidopsis thaliana | - |
silique | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + glycine + H2O | - |
Hordeum vulgare | tetrahydrofolate + L-serine | - |
r | |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
Pisum sativum | tetrahydrofolate + L-serine | - |
r | |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
Arabidopsis thaliana | tetrahydrofolate + L-serine | - |
r | |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
Arabidopsis thaliana | tetrahydrofolate + L-serine | i.e. tetrahydropteroylglutamate, tetrahydropteroylglutamates with more than one glutamate residue are poor substrates and competitive inhibitors, overview | r | |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
Hordeum vulgare Bob | tetrahydrofolate + L-serine | - |
r | |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
Pisum sativum Progress 9 | tetrahydrofolate + L-serine | - |
r |
Synonyms | Comment | Organism |
---|---|---|
AtSHMT3 | - |
Arabidopsis thaliana |
serine hydroxymethyltransferase | - |
Hordeum vulgare |
serine hydroxymethyltransferase | - |
Pisum sativum |
serine hydroxymethyltransferase | - |
Arabidopsis thaliana |
SHMT | - |
Hordeum vulgare |
SHMT | - |
Pisum sativum |
SHMT | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15.8 | - |
tetrahydrofolate | pH 8.5, temperature not specified in the publication, recombinant AtSHMT3 | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Hordeum vulgare |
8.5 | - |
assay at | Pisum sativum |
8.5 | - |
assay at | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on | Hordeum vulgare | |
pyridoxal 5'-phosphate | dependent on | Pisum sativum | |
pyridoxal 5'-phosphate | dependent on | Arabidopsis thaliana |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.085 | - |
tetrahydropteroylglutamate | pH 8.5, temperature not specified in the publication, recombinant AtSHMT3 | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
physiological function | in plastids, SHMTs are thought to catalytically direct the hydroxymethyl moiety of serine into the metabolic network of H4PteGlun-bound one-carbon units | Hordeum vulgare |
physiological function | in plastids, SHMTs are thought to catalytically direct the hydroxymethyl moiety of serine into the metabolic network of H4PteGlun-bound one-carbon units | Pisum sativum |
physiological function | in plastids, SHMTs are thought to catalytically direct the hydroxymethyl moiety of serine into the metabolic network of H4PteGlun-bound one-carbon units | Arabidopsis thaliana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
70 | - |
tetrahydrofolate | pH 8.5, temperature not specified in the publication, recombinant AtSHMT3 | Arabidopsis thaliana |