Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
refolded pyridoxal-5'-phosphate-binding domain of SHMT, gel filtration | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + tetrahydrofolate | Bacillus subtilis | - |
glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
the pyridoxal-5'-phosphate-binding domain of SHMT is purified from inclusion bodies by rapid mixing followed by MonoQ column chromatography | Bacillus subtilis |
Renatured (Comment) | Organism |
---|---|
5 ml of supernatant containing the solubilized domain is added drop by drop to 100 ml of stirring potassium phosphate buffer (50 mM, pH 7.4 containing 1 mM EDTA, 2 mM beta-mercaptoethanol, 0.1 mM pyridoxal 5'-phosphate, and 0.1 M NaCl) | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + tetrahydrofolate | - |
Bacillus subtilis | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | native SHMT | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
serine hydroxymethyltransferase | - |
Bacillus subtilis |
SHMT | - |
Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 65 | a broad sigmoidal transition between 30°C and 65°C having an apparent Tm of about 48°C is observed for the native dimeric SHMT molecule, SHMT is a noncooperative molecule which starts losing the structure from very low temperature (30°C) and loses most of ist secondary structure at relatively high temperature | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Bacillus subtilis |