Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Geobacillus stearothermophilus |
Crystallization (Comment) | Organism |
---|---|
crystals of Y51F and Y61A SHMT mutants are obtained by hanging drop vapour diffusion using 50% (v/v) 2-methyl 2,4-pentanediol as the precipitant | Geobacillus stearothermophilus |
Protein Variants | Comment | Organism |
---|---|---|
Y51F | the mutation results in a complete loss of tetrahydrofolate-dependent and tetrahydrofolate-independent activities | Geobacillus stearothermophilus |
Y61A | the mutation results in a complete loss of tetrahydrofolate-dependent and tetrahydrofolate independent activities | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and DEAE-cellulose column chromatography | Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.05 | - |
mutant enzyme Y61A, using L-serine as substrate, at 37°C | Geobacillus stearothermophilus |
5 | - |
wild type enzyme, using L-serine as substrate, at 37°C | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + tetrahydrofolate | - |
Geobacillus stearothermophilus | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
serine hydroxymethyltransferase | - |
Geobacillus stearothermophilus |
SHMT | - |
Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | wild type enzyme contains 1 mol per mole of subunit | Geobacillus stearothermophilus |