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Literature summary for 2.1.2.1 extracted from

  • Mukherjee, M.; Sievers, S.A.; Brown, M.T.; Johnson, P.J.
    Identification and biochemical characterization of serine hydroxymethyl transferase in the hydrogenosome of Trichomonas vaginalis (2006), Eukaryot. Cell, 5, 2072-2078.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.2.1

Activating Compound

Activating Compound Comment Organism Structure
pyridoxal phosphate
-
 Trichomonas vaginalis

Application

Application Comment Organism
medicine enzyme is distinct from its prokaryotic and eukaryotic counterparts, thus is a potential drug target Trichomonas vaginalis

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutants cloned into vector pET-29B and expressed in Escherichia coli BL21(DE3) cells, moreover expressed in Trichomonas vaginalis with a C-terminal HA tag Trichomonas vaginalis

Protein Variants

Protein Variants Comment Organism
K251Q oligomeric structure not affected, but inactive in both the presence and absence of pyridoxal phosphate Trichomonas vaginalis
K251R oligomeric structure not affected, catalytic activity in the presence of pyridoxal phosphate also unaffected Trichomonas vaginalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 tetrahydrofolate mutant K251R, without pyridoxal phosphate Trichomonas vaginalis
0.89
-
 tetrahydrofolate wild-type, in the presence of 0.25 mM pyridoxal phosphate Trichomonas vaginalis
1
-
 tetrahydrofolate wild-type, without pyridoxal phosphate Trichomonas vaginalis
1.16
-
 tetrahydrofolate mutant K251R, in the presence of 0.25 mM pyridoxal phosphate Trichomonas vaginalis

Localization

Localization Comment Organism GeneOntology No. Textmining
hydrogenosome enzyme possesses a putative N-terminal hydrogenosomal presequence Trichomonas vaginalis 42566
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 sequence analysis Trichomonas vaginalis

Organism

Organism UniProt Comment Textmining
Trichomonas vaginalis
-
 strains T1 and G3
-

Purification (Commentary)

Purification (Comment) Organism
by Ni affinity chromatography, more than 98% pure Trichomonas vaginalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine + tetrahydrofolate
-
 Trichomonas vaginalis glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?

Subunits

Subunits Comment Organism
homodimer immunoblotting Trichomonas vaginalis

Synonyms

Synonyms Comment Organism
serine hydroxymethyl transferase
-
 Trichomonas vaginalis
SHMT
-
 Trichomonas vaginalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.005
-
 tetrahydrofolate mutant K251R, without pyridoxal phosphate Trichomonas vaginalis
1.13
-
 tetrahydrofolate mutant K251R, in the presence of 0.25 mM pyridoxal phosphate Trichomonas vaginalis
1.29
-
 tetrahydrofolate wild-type, without pyridoxal phosphate Trichomonas vaginalis
1.3
-
 tetrahydrofolate wild-type, in the presence of 0.25 mM pyridoxal phosphate Trichomonas vaginalis