Cloned (Comment) | Organism |
---|---|
Escherichia coli DH5alpha-PUC19 and Escherichia coli BL21 (DE3) used as host-vector system | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutant strain 1D19 from first round of DNA-shuffling, shows a 1.7fold increase in SHMT activity, mutant strain 2G31 from the second round of shuffling shows a 2.8fold increase in SHMT activity, mutant strain 3E7 from third round of shuffling, approximately 8fold increased enzyme activity and 41fold increased enzyme productivity as compared with its wild-type parent | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 45000, SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli AB90054 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
gel filtration | Escherichia coli |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
serine hydroxymethyltransferase | - |
Escherichia coli |
SHMT | - |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
for 10 min, the native enzyme conserves about 40% of its activity, while the mutant 3E7 retains about 56% of its activity | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
mutant 3E7 | Escherichia coli |
8 | - |
wild-type | Escherichia coli |