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Literature summary for 2.1.2.1 extracted from

  • Zuo, Z.; Zheng, Z.; Liu, Z.; Yi, Q.; Zou, G.
    Cloning, DNA shuffling and expression of serine hydroxymethyltransferase gene from Escherichia coli strain AB90054 (2007), Enzyme Microb. Technol., 40, 569-577.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
Escherichia coli DH5alpha-PUC19 and Escherichia coli BL21 (DE3) used as host-vector system Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information mutant strain 1D19 from first round of DNA-shuffling, shows a 1.7fold increase in SHMT activity, mutant strain 2G31 from the second round of shuffling shows a 2.8fold increase in SHMT activity, mutant strain 3E7 from third round of shuffling, approximately 8fold increased enzyme activity and 41fold increased enzyme productivity as compared with its wild-type parent Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
x * 45000, SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli AB90054
-
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration Escherichia coli

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
serine hydroxymethyltransferase
-
Escherichia coli
SHMT
-
Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
for 10 min, the native enzyme conserves about 40% of its activity, while the mutant 3E7 retains about 56% of its activity Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
mutant 3E7 Escherichia coli
8
-
wild-type Escherichia coli