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Literature summary for 2.1.2.1 extracted from

  • Sakamoto, M.; Masuda, T.; Yanagimoto, Y.; Nakano, Y.; Kitaoka, S.; Tanigawa, Y.
    Purification and characterization of serine hydroxymethyltransferase from mitochondria of Euglena gracilis z (1996), Biosci. Biotechnol. Biochem., 60, 1941-1944.
No PubMed abstract available
Search for more literature for 2.1.2.1

Inhibitors

Inhibitors Comment Organism Structure
beta-Alanine
-
 Euglena gracilis
glycine
-
 Euglena gracilis
L-alanine
-
 Euglena gracilis
L-alpha,beta-diaminopropionic acid
-
 Euglena gracilis
L-amino acids
-
 Euglena gracilis
L-cysteine
-
 Euglena gracilis
L-methionine not Euglena gracilis
L-serine
-
 Euglena gracilis
additional information not: valine, leucine, glutamic acid, 3-hydroxybutyric acid Euglena gracilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15 0.9 L-serine
-
 Euglena gracilis
65.3
-
 L-serine
-
 Euglena gracilis

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Euglena gracilis 9507
-
cytoplasm
-
 Euglena gracilis 5737
-
mitochondrion
-
 Euglena gracilis 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 2 * 44000, SDS-PAGE Euglena gracilis
88000
-
 gel filtration Euglena gracilis

Organism

Organism UniProt Comment Textmining
Euglena gracilis
-
-
-
Euglena gracilis Z
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Euglena gracilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Euglena gracilis
0.048
-
 reaction of L-serine Euglena gracilis
0.15
-
 reaction of L-threonine Euglena gracilis
1.388
-
 reaction of L-allothreonine Euglena gracilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine + tetrahydrofolate
-
 Euglena gracilis glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?
L-serine + tetrahydrofolate
-
 Euglena gracilis Z glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?
additional information no: D-serine Euglena gracilis ?
-
 ?
additional information no: D-allothreonine Euglena gracilis ?
-
 ?
additional information no: D-threonine Euglena gracilis ?
-
 ?
additional information no: D-serine Euglena gracilis Z ?
-
 ?
additional information no: D-allothreonine Euglena gracilis Z ?
-
 ?
additional information no: D-threonine Euglena gracilis Z ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 44000, SDS-PAGE Euglena gracilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
 Euglena gracilis

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate 2 mol per mol enzyme Euglena gracilis
tetrahydrofolate requirement with L-serine or L-2-methylserine as substrate Euglena gracilis