Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Ovis aries |
Protein Variants | Comment | Organism |
---|---|---|
P297R | 85-90% loss of enzyme activity | Ovis aries |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
tetrahydrofolate | - |
Ovis aries | |
1 | - |
L-serine | - |
Ovis aries | |
2.1 | - |
tetrahydrofolate | mutant enzyme | Ovis aries | |
4 | - |
L-serine | mutant enzyme | Ovis aries |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
210000 | - |
wild-type and mutant enzyme, gelfiltation | Ovis aries |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ovis aries | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.6 | - |
mutant enzyme | Ovis aries |
4.3 | - |
- |
Ovis aries |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + tetrahydrofolate | - |
Ovis aries | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
additional information | enzyme catalyzes the pyridoxal 5'-phosphate dependent reversible cleavage of 3-hydroxy-alpha-amino acids | Ovis aries | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | removal of the bound pyridoxal 5'-phosphate from the mutant tetrameric enzyme leads to dissociation to a dimer | Ovis aries |
homotetramer | - |
Ovis aries |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
Tm of wild-type and mutant enzyme: 54°C, in the presence of serine Tm of wild-type enzyme: 63°C | Ovis aries |
additional information | - |
L-serine increases the thermal stability | Ovis aries |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
L-serine | mutant enzyme | Ovis aries | |
4.2 | - |
L-serine | - |
Ovis aries |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | one mol per subunit | Ovis aries | |
pyridoxal 5'-phosphate | important role in maintaining the structural integrity of the enzyme by preventing the dissociation of the enzyme into subunits, in addition to its function in catalysis | Ovis aries |