Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Carapichea ipecacuanha |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Carapichea ipecacuanha | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38700 | - |
x * 38700, calculated from sequence | Carapichea ipecacuanha |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + cephaeline | Carapichea ipecacuanha | the enzyme is involved in emetine biosynthesis. IpeOMT2 does not catalyze the 6'-O-methylation of cephaeline | S-adenosyl-L-homocysteine + emetine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Carapichea ipecacuanha | D3KY99 | i.e. Psychotria ipecacuanha | - |
Purification (Comment) | Organism |
---|---|
N-terminal His-tagged IpeOMT2 enzyme | Carapichea ipecacuanha |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Carapichea ipecacuanha | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | IpeOMT2 does not catalyze the 6'-O-methylation of cephaeline. IpeOMT2 shows broad substrate specificity towards the intermediates in emetine biosynthesis. The regional substrate specificity for 7'-O-demethylcephaeline (the penultimate step of emetine biosynthesis) is restricted to 7'-O-methylation | Carapichea ipecacuanha | ? | - |
? | |
S-adenosyl-L-methionine + 7'-O-demethylcephaeline | - |
Carapichea ipecacuanha | S-adenosyl-L-homocysteine + cephaeline | - |
? | |
S-adenosyl-L-methionine + cephaeline | the enzyme is involved in emetine biosynthesis. IpeOMT2 does not catalyze the 6'-O-methylation of cephaeline | Carapichea ipecacuanha | S-adenosyl-L-homocysteine + emetine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38700, calculated from sequence | Carapichea ipecacuanha |
Synonyms | Comment | Organism |
---|---|---|
IpeOMT2 | - |
Carapichea ipecacuanha |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Carapichea ipecacuanha |
45 | - |
- |
Carapichea ipecacuanha |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Carapichea ipecacuanha |
7.5 | - |
assay at | Carapichea ipecacuanha |