Cloned (Comment) | Organism |
---|---|
OMT2, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression in Escherichia coli strain BL21(DE3) | Carapichea ipecacuanha |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 53% inhibition at 5 mM | Carapichea ipecacuanha | |
Mn2+ | 31% inhibition at 5 mM | Carapichea ipecacuanha | |
additional information | poor inhibition by iodoacetamide | Carapichea ipecacuanha | |
Ni2+ | 83% inhibition at 5 mM | Carapichea ipecacuanha | |
Zn2+ | 90% inhibition at 5 mM | Carapichea ipecacuanha |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.068 | - |
cephaeline | pH 6.8, 30°C, recombinant CiOMT1 | Carapichea ipecacuanha | |
0.097 | - |
S-adenosyl-L-methionine | pH 6.8, 30°C, recombinant CiOMT1 | Carapichea ipecacuanha |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect by EDTA, Ca2+, and Mg2+ | Carapichea ipecacuanha |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Carapichea ipecacuanha | the enzyme is also active on 7'-O-demethylcephaeline forming cephaeline, overview | ? | - |
? | |
S-adenosyl-L-methionine + cephaeline | Carapichea ipecacuanha | - |
S-adenosyl-L-homocysteine + emetine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Carapichea ipecacuanha | E7FL15 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Carapichea ipecacuanha | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is also active on 7'-O-demethylcephaeline forming cephaeline, overview | Carapichea ipecacuanha | ? | - |
? | |
additional information | substrate specificity of CiOMT2, overview | Carapichea ipecacuanha | ? | - |
? | |
S-adenosyl-L-methionine + cephaeline | - |
Carapichea ipecacuanha | S-adenosyl-L-homocysteine + emetine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CiOMT1 | - |
Carapichea ipecacuanha |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Carapichea ipecacuanha |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Carapichea ipecacuanha |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Carapichea ipecacuanha |
General Information | Comment | Organism |
---|---|---|
metabolism | CiOMT2 catalyzes the penultimate step, 7-O'-methylation, in emetine biosynthesis, the recombinant CiOMT2 also the last step, synthesis of emetine by 6'-O-methylation, pathway overview | Carapichea ipecacuanha |