BRENDA - Enzyme Database show
show all sequences of 2.1.1.B84

Molecular cloning of an O-methyltransferase from adventitious roots of Carapichea ipecacuanha

Cheong, B.E.; Takemura, T.; Yoshimatsu, K.; Sato, F.; Biosci. Biotechnol. Biochem. 75, 107-113 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
OMT2, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression in Escherichia coli strain BL21(DE3)
Carapichea ipecacuanha
Inhibitors
Inhibitors
Commentary
Organism
Structure
Co2+
53% inhibition at 5 mM
Carapichea ipecacuanha
Mn2+
31% inhibition at 5 mM
Carapichea ipecacuanha
additional information
poor inhibition by iodoacetamide
Carapichea ipecacuanha
Ni2+
83% inhibition at 5 mM
Carapichea ipecacuanha
Zn2+
90% inhibition at 5 mM
Carapichea ipecacuanha
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.068
-
cephaeline
pH 6.8, 30°C, recombinant CiOMT1
Carapichea ipecacuanha
0.097
-
S-adenosyl-L-methionine
pH 6.8, 30°C, recombinant CiOMT1
Carapichea ipecacuanha
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no effect by EDTA, Ca2+, and Mg2+
Carapichea ipecacuanha
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Carapichea ipecacuanha
the enzyme is also active on 7'-O-demethylcephaeline forming cephaeline, overview
?
-
-
-
S-adenosyl-L-methionine + cephaeline
Carapichea ipecacuanha
-
S-adenosyl-L-homocysteine + emetine
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Carapichea ipecacuanha
E7FL15
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
root
-
Carapichea ipecacuanha
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the enzyme is also active on 7'-O-demethylcephaeline forming cephaeline, overview
714532
Carapichea ipecacuanha
?
-
-
-
-
additional information
substrate specificity of CiOMT2, overview
714532
Carapichea ipecacuanha
?
-
-
-
-
S-adenosyl-L-methionine + cephaeline
-
714532
Carapichea ipecacuanha
S-adenosyl-L-homocysteine + emetine
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Carapichea ipecacuanha
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
-
assay at
Carapichea ipecacuanha
Cofactor
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Carapichea ipecacuanha
Cloned(Commentary) (protein specific)
Commentary
Organism
OMT2, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression in Escherichia coli strain BL21(DE3)
Carapichea ipecacuanha
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Carapichea ipecacuanha
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Co2+
53% inhibition at 5 mM
Carapichea ipecacuanha
Mn2+
31% inhibition at 5 mM
Carapichea ipecacuanha
additional information
poor inhibition by iodoacetamide
Carapichea ipecacuanha
Ni2+
83% inhibition at 5 mM
Carapichea ipecacuanha
Zn2+
90% inhibition at 5 mM
Carapichea ipecacuanha
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.068
-
cephaeline
pH 6.8, 30°C, recombinant CiOMT1
Carapichea ipecacuanha
0.097
-
S-adenosyl-L-methionine
pH 6.8, 30°C, recombinant CiOMT1
Carapichea ipecacuanha
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no effect by EDTA, Ca2+, and Mg2+
Carapichea ipecacuanha
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Carapichea ipecacuanha
the enzyme is also active on 7'-O-demethylcephaeline forming cephaeline, overview
?
-
-
-
S-adenosyl-L-methionine + cephaeline
Carapichea ipecacuanha
-
S-adenosyl-L-homocysteine + emetine
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
root
-
Carapichea ipecacuanha
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the enzyme is also active on 7'-O-demethylcephaeline forming cephaeline, overview
714532
Carapichea ipecacuanha
?
-
-
-
-
additional information
substrate specificity of CiOMT2, overview
714532
Carapichea ipecacuanha
?
-
-
-
-
S-adenosyl-L-methionine + cephaeline
-
714532
Carapichea ipecacuanha
S-adenosyl-L-homocysteine + emetine
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Carapichea ipecacuanha
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
-
assay at
Carapichea ipecacuanha
General Information
General Information
Commentary
Organism
metabolism
CiOMT2 catalyzes the penultimate step, 7-O'-methylation, in emetine biosynthesis, the recombinant CiOMT2 also the last step, synthesis of emetine by 6'-O-methylation, pathway overview
Carapichea ipecacuanha
General Information (protein specific)
General Information
Commentary
Organism
metabolism
CiOMT2 catalyzes the penultimate step, 7-O'-methylation, in emetine biosynthesis, the recombinant CiOMT2 also the last step, synthesis of emetine by 6'-O-methylation, pathway overview
Carapichea ipecacuanha
Other publictions for EC 2.1.1.B84
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
714532
Cheong
Molecular cloning of an O-meth ...
Carapichea ipecacuanha
Biosci. Biotechnol. Biochem.
75
107-113
2011
-
-
1
-
-
-
5
2
-
1
-
2
-
1
-
-
-
-
-
1
-
-
3
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
5
-
2
-
1
-
2
-
-
-
-
-
1
-
-
3
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
715575
Nomura
Three new O-methyltransferases ...
Carapichea ipecacuanha
J. Biol. Chem.
285
7722-7738
2010
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-
1
-
-
-
-
4
1
-
1
1
-
3
-
-
1
-
-
1
-
-
12
1
2
-
-
4
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
1
-
1
1
-
-
-
1
-
1
-
-
12
1
2
-
-
4
2
-
-
-
-
-
-
-
4
4
720770
Nomura
Is a metabolic enzyme complex ...
Carapichea ipecacuanha
Plant Signal. Behav.
5
875-877
2010
-
-
-
-
-
-
-
-
1
-
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2
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-