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Literature summary for 2.1.1.B84 extracted from
- Molecular cloning of an O-methyltransferase from adventitious roots of Carapichea ipecacuanha (2011), Biosci. Biotechnol. Biochem., 75, 107-113.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| OMT2, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression in Escherichia coli strain BL21(DE3) | Carapichea ipecacuanha |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 53% inhibition at 5 mM | Carapichea ipecacuanha |  |
| Mn2+ | 31% inhibition at 5 mM | Carapichea ipecacuanha | |
| additional information | poor inhibition by iodoacetamide | Carapichea ipecacuanha | |
| Ni2+ | 83% inhibition at 5 mM | Carapichea ipecacuanha | |
| Zn2+ | 90% inhibition at 5 mM | Carapichea ipecacuanha | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.068 | - |
cephaeline | pH 6.8, 30°C, recombinant CiOMT1 | Carapichea ipecacuanha | |
| 0.097 | - |
S-adenosyl-L-methionine | pH 6.8, 30°C, recombinant CiOMT1 | Carapichea ipecacuanha | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no effect by EDTA, Ca2+, and Mg2+ | Carapichea ipecacuanha |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Carapichea ipecacuanha | the enzyme is also active on 7'-O-demethylcephaeline forming cephaeline, overview | ? | - |
? | |
| S-adenosyl-L-methionine + cephaeline | Carapichea ipecacuanha | - |
S-adenosyl-L-homocysteine + emetine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Carapichea ipecacuanha | E7FL15 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | - |
Carapichea ipecacuanha | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme is also active on 7'-O-demethylcephaeline forming cephaeline, overview | Carapichea ipecacuanha | ? | - |
? | |
| additional information | substrate specificity of CiOMT2, overview | Carapichea ipecacuanha | ? | - |
? | |
| S-adenosyl-L-methionine + cephaeline | - |
Carapichea ipecacuanha | S-adenosyl-L-homocysteine + emetine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CiOMT1 | - |
Carapichea ipecacuanha |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Carapichea ipecacuanha |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
assay at | Carapichea ipecacuanha |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosyl-L-methionine | - |
Carapichea ipecacuanha | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | CiOMT2 catalyzes the penultimate step, 7-O'-methylation, in emetine biosynthesis, the recombinant CiOMT2 also the last step, synthesis of emetine by 6'-O-methylation, pathway overview | Carapichea ipecacuanha |
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