BRENDA - Enzyme Database
show all sequences of 2.1.1.B75

High-yield production of multiple O-methylated phenylpropanoids by the engineered Escherichia coli-Streptomyces cocultivation system

Cui, H.; Song, M.; Ban, Y.; Jun, S.; Kwon, A.; Lee, J.; Yoon, Y.; Microb. Cell Fact. 18, 67 (2019)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
OMT2 exhibits more diverse regiospecificity and catalyzes mono-, di-, and tri-methylation of stilbene, flavanone, and flavone when it is expressed in Streptomyces venezuelae compared to expression in Escherichia coli. For the efficient production of multi-methylated phenylpropanoids, a cocultivation system uses engineered Escherichia coli strains producing pterostilbene, naringenin, and apigenin, respectively, along with a OMT2-expressing Streptomyces venezuelae mutant
Streptomyces avermitilis
Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Streptomyces venezuelae
Streptomyces avermitilis
Organism
Organism
UniProt
Commentary
Textmining
Streptomyces avermitilis
Q79ZE8
-
-
Streptomyces avermitilis DSM 46492
Q79ZE8
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
S-adenosyl-L-methionine + apigenin
-
757603
Streptomyces avermitilis
S-adenosyl-L-homocysteine + genkwanin + 5-hydroxy-7,4'-dimethoxyflavone
-
-
-
?
S-adenosyl-L-methionine + apigenin
-
757603
Streptomyces avermitilis DSM 46492
S-adenosyl-L-homocysteine + genkwanin + 5-hydroxy-7,4'-dimethoxyflavone
-
-
-
?
S-adenosyl-L-methionine + naringenin
-
757603
Streptomyces avermitilis
S-adenosyl-L-homocysteine + sakuranetin + 5-hydroxy-7,4'-dimethoxyflavanone + 4'-hydroxy-5,7-dimethoxyflavanone
-
-
-
?
S-adenosyl-L-methionine + naringenin
-
757603
Streptomyces avermitilis DSM 46492
S-adenosyl-L-homocysteine + sakuranetin + 5-hydroxy-7,4'-dimethoxyflavanone + 4'-hydroxy-5,7-dimethoxyflavanone
-
-
-
?
S-adenosyl-L-methionine + resveratrol
-
757603
Streptomyces avermitilis
S-adenosyl-L-homocysteine + desoxyrhapontigenin + 5-hydroxy-3,4'-dimethoxystilbene
-
-
-
?
S-adenosyl-L-methionine + resveratrol
-
757603
Streptomyces avermitilis DSM 46492
S-adenosyl-L-homocysteine + desoxyrhapontigenin + 5-hydroxy-3,4'-dimethoxystilbene
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
OMT2
-
Streptomyces avermitilis
SAVERM_2382
-
Streptomyces avermitilis
Application (protein specific)
Application
Commentary
Organism
synthesis
OMT2 exhibits more diverse regiospecificity and catalyzes mono-, di-, and tri-methylation of stilbene, flavanone, and flavone when it is expressed in Streptomyces venezuelae compared to expression in Escherichia coli. For the efficient production of multi-methylated phenylpropanoids, a cocultivation system uses engineered Escherichia coli strains producing pterostilbene, naringenin, and apigenin, respectively, along with a OMT2-expressing Streptomyces venezuelae mutant
Streptomyces avermitilis
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Streptomyces venezuelae
Streptomyces avermitilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
S-adenosyl-L-methionine + apigenin
-
757603
Streptomyces avermitilis
S-adenosyl-L-homocysteine + genkwanin + 5-hydroxy-7,4'-dimethoxyflavone
-
-
-
?
S-adenosyl-L-methionine + apigenin
-
757603
Streptomyces avermitilis DSM 46492
S-adenosyl-L-homocysteine + genkwanin + 5-hydroxy-7,4'-dimethoxyflavone
-
-
-
?
S-adenosyl-L-methionine + naringenin
-
757603
Streptomyces avermitilis
S-adenosyl-L-homocysteine + sakuranetin + 5-hydroxy-7,4'-dimethoxyflavanone + 4'-hydroxy-5,7-dimethoxyflavanone
-
-
-
?
S-adenosyl-L-methionine + naringenin
-
757603
Streptomyces avermitilis DSM 46492
S-adenosyl-L-homocysteine + sakuranetin + 5-hydroxy-7,4'-dimethoxyflavanone + 4'-hydroxy-5,7-dimethoxyflavanone
-
-
-
?
S-adenosyl-L-methionine + resveratrol
-
757603
Streptomyces avermitilis
S-adenosyl-L-homocysteine + desoxyrhapontigenin + 5-hydroxy-3,4'-dimethoxystilbene
-
-
-
?
S-adenosyl-L-methionine + resveratrol
-
757603
Streptomyces avermitilis DSM 46492
S-adenosyl-L-homocysteine + desoxyrhapontigenin + 5-hydroxy-3,4'-dimethoxystilbene
-
-
-
?
General Information
General Information
Commentary
Organism
physiological function
OMT2 exhibits more diverse regiospecificity and catalyzes mono-, di-, and tri-methylation of stilbene, flavanone, and flavone when it is expressed in Streptomyces venezuelae compared to expression in Escherichia coli. For the efficient production of multi-methylated phenylpropanoids, a cocultivation system uses engineered Escherichia coli strains producing pterostilbene, naringenin, and apigenin, respectively, along with a OMT2-expressing Streptomyces venezuelae mutant
Streptomyces avermitilis
General Information (protein specific)
General Information
Commentary
Organism
physiological function
OMT2 exhibits more diverse regiospecificity and catalyzes mono-, di-, and tri-methylation of stilbene, flavanone, and flavone when it is expressed in Streptomyces venezuelae compared to expression in Escherichia coli. For the efficient production of multi-methylated phenylpropanoids, a cocultivation system uses engineered Escherichia coli strains producing pterostilbene, naringenin, and apigenin, respectively, along with a OMT2-expressing Streptomyces venezuelae mutant
Streptomyces avermitilis
Other publictions for EC 2.1.1.B75
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
757603
Cui
High-yield production of mult ...
Streptomyces avermitilis, Streptomyces avermitilis DSM 46492
Microb. Cell Fact.
18
67
2019
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1
1
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6
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2
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1
1
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6
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1
1
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755826
Parajuli
Substrate scope of O-methyltr ...
Streptomyces peucetius subsp. caesius, Streptomyces peucetius subsp. caesius ATCC 27952
Appl. Biochem. Biotechnol.
184
1404-1420
2018
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1
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6
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2
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2
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9
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2
1
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1
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1
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6
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2
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9
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1
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1
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718652
Malla
Production of 7-O-methyl aroma ...
Streptomyces avermitilis
Appl. Environ. Microbiol.
78
684-694
2012
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1
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1
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1
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4
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2
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1
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1
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1
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1
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-
4
-
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1
1
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713037
Joe
Engineering of flavonoid O-met ...
Populus sp.
Mol. Cells
30
137-141
2010
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1
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1
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4
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2
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1
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1
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4
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2
1
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4
1
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1
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1
1
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1
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4
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2
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1
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4
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1
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4
1
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4
4
674128
Kim
Regiospecific flavonoid 7-O-me ...
Streptomyces avermitilis, Streptomyces avermitilis MA-4680
J. Agric. Food Chem.
54
823-828
2006
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1
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1
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4
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1
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12
1
1
1
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1
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1
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1
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12
1
1
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