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Literature summary for 2.1.1.B75 extracted from

  • Parajuli, P.; Pandey, R.P.; Nguyen, T.H.T.; Dhakal, D.; Sohng, J.K.
    Substrate scope of O-methyltransferase from Streptomyces peucetius for biosynthesis of diverse natural products methoxides (2018), Appl. Biochem. Biotechnol., 184, 1404-1420 .
    View publication on PubMed
Search for more literature for 2.1.1.B75

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces peucetius subsp. caesius

Inhibitors

Inhibitors Comment Organism Structure
Co2+ complete loss of activity Streptomyces peucetius subsp. caesius
Cu2+ complete loss of activity Streptomyces peucetius subsp. caesius
Fe2+ complete loss of activity Streptomyces peucetius subsp. caesius
Mn2+ complete loss of activity Streptomyces peucetius subsp. caesius
additional information no stimulation or inhibition by Ca2+, Ni2+ Streptomyces peucetius subsp. caesius
Zn2+ complete loss of activity Streptomyces peucetius subsp. caesius

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ about 4fold stimulation in presence of MgSO4, about 2.5fold stimulation in presence of MgCl2 Streptomyces peucetius subsp. caesius
additional information no stimulation or inhibition by Ca2+, Ni2+ Streptomyces peucetius subsp. caesius

Organism

Organism UniProt Comment Textmining
Streptomyces peucetius subsp. caesius A0A1B1W6Z7
-
-
Streptomyces peucetius subsp. caesius ATCC 27952 A0A1B1W6Z7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-adenosyl-L-methionine + aloe emodin
-
 Streptomyces peucetius subsp. caesius 2 S-adenosyl-L-homocysteine + O-methylaloe emodin
-
 ?
2 S-adenosyl-L-methionine + beta-sitosterol
-
 Streptomyces peucetius subsp. caesius 2 S-adenosyl-L-homocysteine + O-methyl-beta-sitosterol
-
 ?
2 S-adenosyl-L-methionine + daunorubicin
-
 Streptomyces peucetius subsp. caesius 2 S-adenosyl-L-homocysteine + O-methyldaunorubicin
-
 ?
2 S-adenosyl-L-methionine + emodin
-
 Streptomyces peucetius subsp. caesius 2 S-adenosyl-L-homocysteine + O-methylemodin
-
 ?
2 S-adenosyl-L-methionine + genistein
-
 Streptomyces peucetius subsp. caesius 2 S-adenosyl-L-homocysteine + O-methylgenistein + O,O'-dimethylgenistein + O,O',O''-trimethylgenistein
-
 ?
2 S-adenosyl-L-methionine + luteolin
-
 Streptomyces peucetius subsp. caesius 2 S-adenosyl-L-homocysteine + methoxyluteolin
-
 ?
2 S-adenosyl-L-methionine + phloretin
-
 Streptomyces peucetius subsp. caesius 2 S-adenosyl-L-homocysteine + methoxyphloretin
-
 ?
S-adenosyl-L-methionine + 3-hydroxyflavone
-
 Streptomyces peucetius subsp. caesius S-adenosyl-L-homocysteine + 3-methoxyflavone
-
 ?
S-adenosyl-L-methionine + 7,8-dihydroxyflavone
-
 Streptomyces peucetius subsp. caesius S-adenosyl-L-homocysteine + 7-hydroxy-8-methoxyflavone
-
 ?

Synonyms

Synonyms Comment Organism
CGZ69_28180
-
 Streptomyces peucetius subsp. caesius
SpOMT7740
-
 Streptomyces peucetius subsp. caesius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Streptomyces peucetius subsp. caesius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Streptomyces peucetius subsp. caesius