BRENDA - Enzyme Database
show all sequences of 2.1.1.B75

Substrate scope of O-methyltransferase from Streptomyces peucetius for biosynthesis of diverse natural products methoxides

Parajuli, P.; Pandey, R.P.; Nguyen, T.H.T.; Dhakal, D.; Sohng, J.K.; Appl. Biochem. Biotechnol. 184, 1404-1420 (2018)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Streptomyces peucetius subsp. caesius
Inhibitors
Inhibitors
Commentary
Organism
Structure
Co2+
complete loss of activity
Streptomyces peucetius subsp. caesius
Cu2+
complete loss of activity
Streptomyces peucetius subsp. caesius
Fe2+
complete loss of activity
Streptomyces peucetius subsp. caesius
Mn2+
complete loss of activity
Streptomyces peucetius subsp. caesius
additional information
no stimulation or inhibition by Ca2+, Ni2+
Streptomyces peucetius subsp. caesius
Zn2+
complete loss of activity
Streptomyces peucetius subsp. caesius
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
about 4fold stimulation in presence of MgSO4, about 2.5fold stimulation in presence of MgCl2
Streptomyces peucetius subsp. caesius
additional information
no stimulation or inhibition by Ca2+, Ni2+
Streptomyces peucetius subsp. caesius
Organism
Organism
UniProt
Commentary
Textmining
Streptomyces peucetius subsp. caesius
A0A1B1W6Z7
-
-
Streptomyces peucetius subsp. caesius ATCC 27952
A0A1B1W6Z7
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2 S-adenosyl-L-methionine + 3-hydroxyflavone
-
755826
Streptomyces peucetius subsp. caesius
2 S-adenosyl-L-homocysteine + 3-methoxyflavone
-
-
-
?
2 S-adenosyl-L-methionine + 7,8-dihydroxyflavone
-
755826
Streptomyces peucetius subsp. caesius
2 S-adenosyl-L-homocysteine + 7-hydroxy-8-methoxyflavone
-
-
-
?
2 S-adenosyl-L-methionine + aloe emodin
-
755826
Streptomyces peucetius subsp. caesius
2 S-adenosyl-L-homocysteine + O-methylaloe emodin
-
-
-
?
2 S-adenosyl-L-methionine + daunorubicin
-
755826
Streptomyces peucetius subsp. caesius
2 S-adenosyl-L-homocysteine + O-methyldaunorubicin
-
-
-
?
2 S-adenosyl-L-methionine + emodin
-
755826
Streptomyces peucetius subsp. caesius
2 S-adenosyl-L-homocysteine + O-methylemodin
-
-
-
?
2 S-adenosyl-L-methionine + genistein
-
755826
Streptomyces peucetius subsp. caesius
2 S-adenosyl-L-homocysteine + O-methylgenistein + O,O'-dimethylgenistein + O,O',O''-trimethylgenistein
-
-
-
?
2 S-adenosyl-L-methionine + luteolin
-
755826
Streptomyces peucetius subsp. caesius
2 S-adenosyl-L-homocysteine + methoxyluteolin
-
-
-
?
2 S-adenosyl-L-methionine + phoretin
-
755826
Streptomyces peucetius subsp. caesius
2 S-adenosyl-L-homocysteine + methoxyphloretin
-
-
-
?
S: 2 S-adenosyl-L-methionine + beta-sitosterol
-
755826
Streptomyces peucetius subsp. caesius
2 S-adenosyl-L-homocysteine + O-methyl-beta-sitosterol
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
CGZ69_28180
-
Streptomyces peucetius subsp. caesius
SpOMT7740
-
Streptomyces peucetius subsp. caesius
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Streptomyces peucetius subsp. caesius
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Streptomyces peucetius subsp. caesius
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Streptomyces peucetius subsp. caesius
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Co2+
complete loss of activity
Streptomyces peucetius subsp. caesius
Cu2+
complete loss of activity
Streptomyces peucetius subsp. caesius
Fe2+
complete loss of activity
Streptomyces peucetius subsp. caesius
Mn2+
complete loss of activity
Streptomyces peucetius subsp. caesius
additional information
no stimulation or inhibition by Ca2+, Ni2+
Streptomyces peucetius subsp. caesius
Zn2+
complete loss of activity
Streptomyces peucetius subsp. caesius
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
about 4fold stimulation in presence of MgSO4, about 2.5fold stimulation in presence of MgCl2
Streptomyces peucetius subsp. caesius
additional information
no stimulation or inhibition by Ca2+, Ni2+
Streptomyces peucetius subsp. caesius
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2 S-adenosyl-L-methionine + 3-hydroxyflavone
-
755826
Streptomyces peucetius subsp. caesius
2 S-adenosyl-L-homocysteine + 3-methoxyflavone
-
-
-
?
2 S-adenosyl-L-methionine + 7,8-dihydroxyflavone
-
755826
Streptomyces peucetius subsp. caesius
2 S-adenosyl-L-homocysteine + 7-hydroxy-8-methoxyflavone
-
-
-
?
2 S-adenosyl-L-methionine + aloe emodin
-
755826
Streptomyces peucetius subsp. caesius
2 S-adenosyl-L-homocysteine + O-methylaloe emodin
-
-
-
?
2 S-adenosyl-L-methionine + daunorubicin
-
755826
Streptomyces peucetius subsp. caesius
2 S-adenosyl-L-homocysteine + O-methyldaunorubicin
-
-
-
?
2 S-adenosyl-L-methionine + emodin
-
755826
Streptomyces peucetius subsp. caesius
2 S-adenosyl-L-homocysteine + O-methylemodin
-
-
-
?
2 S-adenosyl-L-methionine + genistein
-
755826
Streptomyces peucetius subsp. caesius
2 S-adenosyl-L-homocysteine + O-methylgenistein + O,O'-dimethylgenistein + O,O',O''-trimethylgenistein
-
-
-
?
2 S-adenosyl-L-methionine + luteolin
-
755826
Streptomyces peucetius subsp. caesius
2 S-adenosyl-L-homocysteine + methoxyluteolin
-
-
-
?
2 S-adenosyl-L-methionine + phoretin
-
755826
Streptomyces peucetius subsp. caesius
2 S-adenosyl-L-homocysteine + methoxyphloretin
-
-
-
?
S: 2 S-adenosyl-L-methionine + beta-sitosterol
-
755826
Streptomyces peucetius subsp. caesius
2 S-adenosyl-L-homocysteine + O-methyl-beta-sitosterol
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Streptomyces peucetius subsp. caesius
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Streptomyces peucetius subsp. caesius
Other publictions for EC 2.1.1.B75
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
757603
Cui
High-yield production of mult ...
Streptomyces avermitilis, Streptomyces avermitilis DSM 46492
Microb. Cell Fact.
18
67
2019
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
6
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
755826
Parajuli
Substrate scope of O-methyltr ...
Streptomyces peucetius subsp. caesius, Streptomyces peucetius subsp. caesius ATCC 27952
Appl. Biochem. Biotechnol.
184
1404-1420
2018
-
-
1
-
-
-
6
-
-
2
-
-
-
2
-
-
-
-
-
-
-
-
9
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
6
-
-
-
2
-
-
-
-
-
-
-
-
-
-
9
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
718652
Malla
Production of 7-O-methyl aroma ...
Streptomyces avermitilis
Appl. Environ. Microbiol.
78
684-694
2012
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
713037
Joe
Engineering of flavonoid O-met ...
Populus sp.
Mol. Cells
30
137-141
2010
-
-
1
-
1
-
-
4
-
-
-
2
-
1
-
-
1
-
-
-
-
-
4
-
2
1
-
-
4
1
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
4
-
-
-
2
-
-
-
1
-
-
-
-
4
-
1
-
-
4
1
-
-
-
-
-
-
-
4
4
674128
Kim
Regiospecific flavonoid 7-O-me ...
Streptomyces avermitilis, Streptomyces avermitilis MA-4680
J. Agric. Food Chem.
54
823-828
2006
-
-
1
-
-
-
-
-
-
-
1
-
-
4
-
-
1
-
-
-
-
-
12
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
12
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-