Substrate scope of O-methyltransferase from Streptomyces peucetius for biosynthesis of diverse natural products methoxides

Parajuli, P.; Pandey, R.P.; Nguyen, T.H.T.; Dhakal, D.; Sohng, J.K.; Appl. Biochem. Biotechnol. 184, 1404-1420 (2018)

Data extracted from this reference:

Inhibitors

Commentary

Organism

Structure

Co2+

complete loss of activity

Streptomyces peucetius subsp. caesius

Cu2+

complete loss of activity

Streptomyces peucetius subsp. caesius

Fe2+

complete loss of activity

Streptomyces peucetius subsp. caesius

Mn2+

complete loss of activity

Streptomyces peucetius subsp. caesius

additional information

no stimulation or inhibition by Ca2+, Ni2+

Streptomyces peucetius subsp. caesius

Zn2+

complete loss of activity

Streptomyces peucetius subsp. caesius

Metals/Ions

Commentary

Organism

Structure

Mg2+

about 4fold stimulation in presence of MgSO4, about 2.5fold stimulation in presence of MgCl2

Streptomyces peucetius subsp. caesius

additional information

no stimulation or inhibition by Ca2+, Ni2+

Streptomyces peucetius subsp. caesius

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

2 S-adenosyl-L-methionine + 3-hydroxyflavone

-

755826

Streptomyces peucetius subsp. caesius

2 S-adenosyl-L-homocysteine + 3-methoxyflavone

-

-

-

?

2 S-adenosyl-L-methionine + 7,8-dihydroxyflavone

-

755826

Streptomyces peucetius subsp. caesius

2 S-adenosyl-L-homocysteine + 7-hydroxy-8-methoxyflavone

-

-

-

?

2 S-adenosyl-L-methionine + aloe emodin

-

755826

Streptomyces peucetius subsp. caesius

2 S-adenosyl-L-homocysteine + O-methylaloe emodin

-

-

-

?

2 S-adenosyl-L-methionine + daunorubicin

-

755826

Streptomyces peucetius subsp. caesius

2 S-adenosyl-L-homocysteine + O-methyldaunorubicin

-

-

-

?

2 S-adenosyl-L-methionine + emodin

-

755826

Streptomyces peucetius subsp. caesius

2 S-adenosyl-L-homocysteine + O-methylemodin

-

-

-

?

2 S-adenosyl-L-methionine + genistein

-

755826

Streptomyces peucetius subsp. caesius

2 S-adenosyl-L-homocysteine + O-methylgenistein + O,O'-dimethylgenistein + O,O',O''-trimethylgenistein

-

-

-

?

2 S-adenosyl-L-methionine + luteolin

-

755826

Streptomyces peucetius subsp. caesius

2 S-adenosyl-L-homocysteine + methoxyluteolin

-

-

-

?

2 S-adenosyl-L-methionine + phoretin

-

755826

Streptomyces peucetius subsp. caesius

2 S-adenosyl-L-homocysteine + methoxyphloretin

-

-

-

?

S: 2 S-adenosyl-L-methionine + beta-sitosterol

-

755826

Streptomyces peucetius subsp. caesius

2 S-adenosyl-L-homocysteine + O-methyl-beta-sitosterol

-

-

-

?

Inhibitors

Commentary

Organism

Structure

Co2+

complete loss of activity

Streptomyces peucetius subsp. caesius

Cu2+

complete loss of activity

Streptomyces peucetius subsp. caesius

Fe2+

complete loss of activity

Streptomyces peucetius subsp. caesius

Mn2+

complete loss of activity

Streptomyces peucetius subsp. caesius

additional information

no stimulation or inhibition by Ca2+, Ni2+

Streptomyces peucetius subsp. caesius

Zn2+

complete loss of activity

Streptomyces peucetius subsp. caesius

Metals/Ions

Commentary

Organism

Structure

Mg2+

about 4fold stimulation in presence of MgSO4, about 2.5fold stimulation in presence of MgCl2

Streptomyces peucetius subsp. caesius

additional information

no stimulation or inhibition by Ca2+, Ni2+

Streptomyces peucetius subsp. caesius

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

2 S-adenosyl-L-methionine + 3-hydroxyflavone

-

755826

Streptomyces peucetius subsp. caesius

2 S-adenosyl-L-homocysteine + 3-methoxyflavone

-

-

-

?

2 S-adenosyl-L-methionine + 7,8-dihydroxyflavone

-

755826

Streptomyces peucetius subsp. caesius

2 S-adenosyl-L-homocysteine + 7-hydroxy-8-methoxyflavone

-

-

-

?

2 S-adenosyl-L-methionine + aloe emodin

-

755826

Streptomyces peucetius subsp. caesius

2 S-adenosyl-L-homocysteine + O-methylaloe emodin

-

-

-

?

2 S-adenosyl-L-methionine + daunorubicin

-

755826

Streptomyces peucetius subsp. caesius

2 S-adenosyl-L-homocysteine + O-methyldaunorubicin

-

-

-

?

2 S-adenosyl-L-methionine + emodin

-

755826

Streptomyces peucetius subsp. caesius

2 S-adenosyl-L-homocysteine + O-methylemodin

-

-

-

?

2 S-adenosyl-L-methionine + genistein

-

755826

Streptomyces peucetius subsp. caesius

2 S-adenosyl-L-homocysteine + O-methylgenistein + O,O'-dimethylgenistein + O,O',O''-trimethylgenistein

-

-

-

?

2 S-adenosyl-L-methionine + luteolin

-

755826

Streptomyces peucetius subsp. caesius

2 S-adenosyl-L-homocysteine + methoxyluteolin

-

-

-

?

2 S-adenosyl-L-methionine + phoretin

-

755826

Streptomyces peucetius subsp. caesius

2 S-adenosyl-L-homocysteine + methoxyphloretin

-

-

-

?

S: 2 S-adenosyl-L-methionine + beta-sitosterol

-

755826

Streptomyces peucetius subsp. caesius

2 S-adenosyl-L-homocysteine + O-methyl-beta-sitosterol

-

-

-

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

757603

Cui

High-yield production of mult ...

Streptomyces avermitilis, Streptomyces avermitilis DSM 46492

Microb. Cell Fact.

18

67

2019

-

1

1

-

-

-

-

-

-

-

-

-

-

2

-

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6

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2

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1

1

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-

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-

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-

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6

-

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-

-

-

-

-

1

1

-

-

-

755826

Parajuli

Substrate scope of O-methyltr ...

Streptomyces peucetius subsp. caesius, Streptomyces peucetius subsp. caesius ATCC 27952

Appl. Biochem. Biotechnol.

184

1404-1420

2018

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1

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6

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2

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9

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2

1

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1

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6

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9

-

1

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1

-

-

-

-

-

-

-

-

-

718652

Malla

Production of 7-O-methyl aroma ...

Streptomyces avermitilis

Appl. Environ. Microbiol.

78

684-694

2012

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1

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1

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1

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4

-

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-

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1

1

-

-

-

713037

Joe

Engineering of flavonoid O-met ...

Populus sp.

Mol. Cells

30

137-141

2010

-

-

1

-

1

-

-

4

-

-

-

2

-

1

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1

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4

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2

1

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1

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1

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4

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1

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4

1

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-

-

-

-

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4

4

674128

Kim

Regiospecific flavonoid 7-O-me ...

Streptomyces avermitilis, Streptomyces avermitilis MA-4680

J. Agric. Food Chem.

54

823-828

2006

-

-

1

-

-

-

-

-

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1

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4

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1

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12

1

1

1

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1

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1

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1

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12

1

1

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