Data extracted from this reference:
Cloned(Commentary)
gene POMT7, error-prone polymerase chain reaction, expression of GST-tagged wild-type POMT7 and mutant POMT7-M1 in Escherichia coli
Populus sp.
Engineering
D257G
random mutagenesis, the mutant of POMT-7, POMT7-M1, has a novel regioselectivity, from large scale mutant screening, mutant D257G methylates the 3-hydroxyl group of flavonols in addition to 7-hydrdoxyl group
Populus sp.
KM Value [mM]
0.0298
kaempferol
pH 7.5, 37°C, recombinant wild-type POMT7
Populus sp.
0.0324
kaempferol
pH 7.5, 37°C, recombinant mutant POMT7-M1
Populus sp.
0.0324
quercetin
pH 7.5, 37°C, recombinant wild-type POMT7
Populus sp.
0.0378
quercetin
pH 7.5, 37°C, recombinant mutant POMT7-M1
Populus sp.
Natural Substrates/ Products (Substrates)
S-adenosyl-L-methionine + kaempferol
Populus sp.
S-adenosyl-L-homocysteine + 7-O-methylkaempferol
?
S-adenosyl-L-methionine + quercetin
Populus sp.
S-adenosyl-L-homocysteine + 7-O-methylquercetin
?
Organism
Purification (Commentary)
recombinant GST-tagged wild-type POMT7 and mutant POMT7-M1 fromEscherichia coli by glutathione affinity chromatography
Populus sp.
Substrates and Products (Substrate)
2 S-adenosyl-L-methionine + kaempferol
activity only of enzyme mutant POMT7-M1, D257G, not of wild-type POMT7
713037
Populus sp.
2 S-adenosyl-L-homocysteine + 3,7-O-dimethylkaempferol
?
2 S-adenosyl-L-methionine + quercetin
activity only of enzyme mutant POMT7-M1, D257G, not of wild-type POMT7, molecular docking of quercetin into the modeled structure of Asp257Gly
713037
Populus sp.
2 S-adenosyl-L-homocysteine + 3,7-O-dimethylquercetin
?
S-adenosyl-L-methionine + kaempferol
713037
Populus sp.
S-adenosyl-L-homocysteine + 7-O-methylkaempferol
?
S-adenosyl-L-methionine + quercetin
713037
Populus sp.
S-adenosyl-L-homocysteine + 7-O-methylquercetin
?
Synonyms
flavonoid O-methyltransferase
Populus sp.
Temperature Optimum [°C]
Turnover Number [1/s]
0.064
kaempferol
pH 7.5, 37°C, recombinant mutant POMT7-M1
Populus sp.
0.064
quercetin
pH 7.5, 37°C, recombinant wild-type POMT7
Populus sp.
0.07
quercetin
pH 7.5, 37°C, recombinant mutant POMT7-M1
Populus sp.
0.071
kaempferol
pH 7.5, 37°C, recombinant wild-type POMT7
Populus sp.
pH Optimum
Cofactor
S-adenosyl-L-methionine
Populus sp.
Cloned(Commentary) (protein specific)
gene POMT7, error-prone polymerase chain reaction, expression of GST-tagged wild-type POMT7 and mutant POMT7-M1 in Escherichia coli
Populus sp.
Cofactor (protein specific)
S-adenosyl-L-methionine
Populus sp.
Engineering (protein specific)
D257G
random mutagenesis, the mutant of POMT-7, POMT7-M1, has a novel regioselectivity, from large scale mutant screening, mutant D257G methylates the 3-hydroxyl group of flavonols in addition to 7-hydrdoxyl group
Populus sp.
KM Value [mM] (protein specific)
0.0298
kaempferol
pH 7.5, 37°C, recombinant wild-type POMT7
Populus sp.
0.0324
kaempferol
pH 7.5, 37°C, recombinant mutant POMT7-M1
Populus sp.
0.0324
quercetin
pH 7.5, 37°C, recombinant wild-type POMT7
Populus sp.
0.0378
quercetin
pH 7.5, 37°C, recombinant mutant POMT7-M1
Populus sp.
Natural Substrates/ Products (Substrates) (protein specific)
S-adenosyl-L-methionine + kaempferol
Populus sp.
S-adenosyl-L-homocysteine + 7-O-methylkaempferol
?
S-adenosyl-L-methionine + quercetin
Populus sp.
S-adenosyl-L-homocysteine + 7-O-methylquercetin
?
Purification (Commentary) (protein specific)
recombinant GST-tagged wild-type POMT7 and mutant POMT7-M1 fromEscherichia coli by glutathione affinity chromatography
Populus sp.
Substrates and Products (Substrate) (protein specific)
2 S-adenosyl-L-methionine + kaempferol
activity only of enzyme mutant POMT7-M1, D257G, not of wild-type POMT7
713037
Populus sp.
2 S-adenosyl-L-homocysteine + 3,7-O-dimethylkaempferol
?
2 S-adenosyl-L-methionine + quercetin
activity only of enzyme mutant POMT7-M1, D257G, not of wild-type POMT7, molecular docking of quercetin into the modeled structure of Asp257Gly
713037
Populus sp.
2 S-adenosyl-L-homocysteine + 3,7-O-dimethylquercetin
?
S-adenosyl-L-methionine + kaempferol
713037
Populus sp.
S-adenosyl-L-homocysteine + 7-O-methylkaempferol
?
S-adenosyl-L-methionine + quercetin
713037
Populus sp.
S-adenosyl-L-homocysteine + 7-O-methylquercetin
?
Temperature Optimum [°C] (protein specific)
Turnover Number [1/s] (protein specific)
0.064
kaempferol
pH 7.5, 37°C, recombinant mutant POMT7-M1
Populus sp.
0.064
quercetin
pH 7.5, 37°C, recombinant wild-type POMT7
Populus sp.
0.07
quercetin
pH 7.5, 37°C, recombinant mutant POMT7-M1
Populus sp.
0.071
kaempferol
pH 7.5, 37°C, recombinant wild-type POMT7
Populus sp.
pH Optimum (protein specific)
KCat/KM [mM/s]
1.7
quercetin
pH 7.5, 37°C, recombinant mutant POMT7-M1
Populus sp.
1.9
kaempferol
pH 7.5, 37°C, recombinant mutant POMT7-M1
Populus sp.
1.9
quercetin
pH 7.5, 37°C, recombinant wild-type POMT7
Populus sp.
2.4
kaempferol
pH 7.5, 37°C, recombinant wild-type POMT7
Populus sp.
KCat/KM [mM/s] (protein specific)
1.7
quercetin
pH 7.5, 37°C, recombinant mutant POMT7-M1
Populus sp.
1.9
kaempferol
pH 7.5, 37°C, recombinant mutant POMT7-M1
Populus sp.
1.9
quercetin
pH 7.5, 37°C, recombinant wild-type POMT7
Populus sp.
2.4
kaempferol
pH 7.5, 37°C, recombinant wild-type POMT7
Populus sp.
Other publictions for EC 2.1.1.B75
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Production of 7-O-methyl aroma ...
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Engineering of flavonoid O-met ...
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