Engineering of flavonoid O-methyltransferase for a novel regioselectivity
Joe, E.J.; Kim, B.G.; An, B.C.; Chong, Y.; Ahn, J.H.; Mol. Cells 30, 137-141 (2010) 
Data extracted from this reference:
Cloned(Commentary)
Cloned (Commentary)
Organism
gene POMT7, error-prone polymerase chain reaction, expression of GST-tagged wild-type POMT7 and mutant POMT7-M1 in Escherichia coli
Populus sp.
Engineering
Protein Variants
Commentary
Organism
D257G
random mutagenesis, the mutant of POMT-7, POMT7-M1, has a novel regioselectivity, from large scale mutant screening, mutant D257G methylates the 3-hydroxyl group of flavonols in addition to 7-hydrdoxyl group
Populus sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0298
-
kaempferol
pH 7.5, 37°C, recombinant wild-type POMT7
Populus sp.
0.0324
-
kaempferol
pH 7.5, 37°C, recombinant mutant POMT7-M1
Populus sp.
0.0324
-
quercetin
pH 7.5, 37°C, recombinant wild-type POMT7
Populus sp.
0.0378
-
quercetin
pH 7.5, 37°C, recombinant mutant POMT7-M1
Populus sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
S-adenosyl-L-methionine + kaempferol
Populus sp.
-
S-adenosyl-L-homocysteine + 7-O-methylkaempferol
-
-
?
S-adenosyl-L-methionine + quercetin
Populus sp.
-
S-adenosyl-L-homocysteine + 7-O-methylquercetin
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Populus sp.
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
recombinant GST-tagged wild-type POMT7 and mutant POMT7-M1 fromEscherichia coli by glutathione affinity chromatography
Populus sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2 S-adenosyl-L-methionine + kaempferol
activity only of enzyme mutant POMT7-M1, D257G, not of wild-type POMT7
713037
Populus sp.
2 S-adenosyl-L-homocysteine + 3,7-O-dimethylkaempferol
-
-
-
?
2 S-adenosyl-L-methionine + quercetin
activity only of enzyme mutant POMT7-M1, D257G, not of wild-type POMT7, molecular docking of quercetin into the modeled structure of Asp257Gly
713037
Populus sp.
2 S-adenosyl-L-homocysteine + 3,7-O-dimethylquercetin
-
-
-
?
S-adenosyl-L-methionine + kaempferol
-
713037
Populus sp.
S-adenosyl-L-homocysteine + 7-O-methylkaempferol
-
-
-
?
S-adenosyl-L-methionine + quercetin
-
713037
Populus sp.
S-adenosyl-L-homocysteine + 7-O-methylquercetin
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
flavonoid O-methyltransferase
-
Populus sp.
POMT7
-
Populus sp.
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Populus sp.
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.064
-
kaempferol
pH 7.5, 37°C, recombinant mutant POMT7-M1
Populus sp.
0.064
-
quercetin
pH 7.5, 37°C, recombinant wild-type POMT7
Populus sp.
0.07
-
quercetin
pH 7.5, 37°C, recombinant mutant POMT7-M1
Populus sp.
0.071
-
kaempferol
pH 7.5, 37°C, recombinant wild-type POMT7
Populus sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Populus sp.
Cofactor
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Populus sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
gene POMT7, error-prone polymerase chain reaction, expression of GST-tagged wild-type POMT7 and mutant POMT7-M1 in Escherichia coli
Populus sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Populus sp.
Engineering (protein specific)
Protein Variants
Commentary
Organism
D257G
random mutagenesis, the mutant of POMT-7, POMT7-M1, has a novel regioselectivity, from large scale mutant screening, mutant D257G methylates the 3-hydroxyl group of flavonols in addition to 7-hydrdoxyl group
Populus sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0298
-
kaempferol
pH 7.5, 37°C, recombinant wild-type POMT7
Populus sp.
0.0324
-
kaempferol
pH 7.5, 37°C, recombinant mutant POMT7-M1
Populus sp.
0.0324
-
quercetin
pH 7.5, 37°C, recombinant wild-type POMT7
Populus sp.
0.0378
-
quercetin
pH 7.5, 37°C, recombinant mutant POMT7-M1
Populus sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
S-adenosyl-L-methionine + kaempferol
Populus sp.
-
S-adenosyl-L-homocysteine + 7-O-methylkaempferol
-
-
?
S-adenosyl-L-methionine + quercetin
Populus sp.
-
S-adenosyl-L-homocysteine + 7-O-methylquercetin
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant GST-tagged wild-type POMT7 and mutant POMT7-M1 fromEscherichia coli by glutathione affinity chromatography
Populus sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2 S-adenosyl-L-methionine + kaempferol
activity only of enzyme mutant POMT7-M1, D257G, not of wild-type POMT7
713037
Populus sp.
2 S-adenosyl-L-homocysteine + 3,7-O-dimethylkaempferol
-
-
-
?
2 S-adenosyl-L-methionine + quercetin
activity only of enzyme mutant POMT7-M1, D257G, not of wild-type POMT7, molecular docking of quercetin into the modeled structure of Asp257Gly
713037
Populus sp.
2 S-adenosyl-L-homocysteine + 3,7-O-dimethylquercetin
-
-
-
?
S-adenosyl-L-methionine + kaempferol
-
713037
Populus sp.
S-adenosyl-L-homocysteine + 7-O-methylkaempferol
-
-
-
?
S-adenosyl-L-methionine + quercetin
-
713037
Populus sp.
S-adenosyl-L-homocysteine + 7-O-methylquercetin
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Populus sp.
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.064
-
kaempferol
pH 7.5, 37°C, recombinant mutant POMT7-M1
Populus sp.
0.064
-
quercetin
pH 7.5, 37°C, recombinant wild-type POMT7
Populus sp.
0.07
-
quercetin
pH 7.5, 37°C, recombinant mutant POMT7-M1
Populus sp.
0.071
-
kaempferol
pH 7.5, 37°C, recombinant wild-type POMT7
Populus sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Populus sp.
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.7
-
quercetin
pH 7.5, 37°C, recombinant mutant POMT7-M1
Populus sp.
1.9
-
kaempferol
pH 7.5, 37°C, recombinant mutant POMT7-M1
Populus sp.
1.9
-
quercetin
pH 7.5, 37°C, recombinant wild-type POMT7
Populus sp.
2.4
-
kaempferol
pH 7.5, 37°C, recombinant wild-type POMT7
Populus sp.
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.7
-
quercetin
pH 7.5, 37°C, recombinant mutant POMT7-M1
Populus sp.
1.9
-
kaempferol
pH 7.5, 37°C, recombinant mutant POMT7-M1
Populus sp.
1.9
-
quercetin
pH 7.5, 37°C, recombinant wild-type POMT7
Populus sp.
2.4
-
kaempferol
pH 7.5, 37°C, recombinant wild-type POMT7
Populus sp.
Other publictions for EC 2.1.1.B75
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
718652
Malla
Production of 7-O-methyl aroma ...
Streptomyces avermitilis
Appl. Environ. Microbiol.
78
684-694
2012
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
713037
Joe
Engineering of flavonoid O-met ...
Populus sp.
Mol. Cells
30
137-141
2010
-
-
1
-
1
-
-
4
-
-
-
2
-
1
-
-
1
-
-
-
-
-
4
-
2
1
-
-
4
1
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
4
-
-
-
2
-
-
-
1
-
-
-
-
4
-
1
-
-
4
1
-
-
-
-
-
-
-
4
4
674128
Kim
Regiospecific flavonoid 7-O-me ...
Streptomyces avermitilis, Streptomyces avermitilis MA-4680
J. Agric. Food Chem.
54
823-828
2006
-
-
1
-
-
-
-
-
-
-
1
-
-
4
-
-
1
-
-
-
-
-
12
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
12
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-