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Literature summary for 2.1.1.B74 extracted from

  • Christensen, A.B.; Gregersen, P.L.; Olsen, C.E.; Collinge, D.B.
    A flavonoid 7-O-methyltransferase is expressed in barley leaves in response to pathogen attack (1998), Plant Mol. Biol., 36, 219-227.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Hordeum vulgare

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0046
-
5,7,4'-trihydroxyflavone pH 7.8, 20°C Hordeum vulgare
0.0109
-
S-adenosyl-L-methionine pH 7.8, 20°C Hordeum vulgare

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42300
-
calculated from sequence Hordeum vulgare

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 5,7,4'-trihydroxyflavone Hordeum vulgare i.e. apigenin. The product genkwanin exhibits a stronger inhibitory effect on the germination of spores from pathogenic fungi as compared to the effect of apigenin S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyflavone i.e. genkwanin ?

Organism

Organism UniProt Comment Textmining
Hordeum vulgare Q43771 inoculated with the pathogenic fungus Blumeria graminis
-

Purification (Commentary)

Purification (Comment) Organism
-
Hordeum vulgare

Source Tissue

Source Tissue Comment Organism Textmining
kernel
-
Hordeum vulgare
-
leaf expressed in barley leaves in response to attack by the pathogenic fungus Blumeria graminis. NOt detected in healthy plants Hordeum vulgare
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 3',4',5,7-tetrahydroxyflavanone i.e. eriodictyol, about 10% of the activity with 5,7,4'-trihydroxyflavone (i.e. apigenin) Hordeum vulgare S-adenosyl-L-homocysteine + 3',4',5-trihydroxy-7-methoxyflavanone
-
?
S-adenosyl-L-methionine + 3,4',5,7-tetrahydroxyflavanone i.e. kaempferol, about 15% of the activity with 5,7,4'-trihydroxyflavone (i.e. apigenin) Hordeum vulgare S-adenosyl-L-homocysteine + 3,4',5-trihydroxy-7-methoxyflavanone
-
?
S-adenosyl-L-methionine + 5,7,3',4'-tetrahydroxyflavone i.e. luteolin, about 15% of the activity with 5,7,4'-trihydroxyflavone (i.e. apigenin) Hordeum vulgare S-adenosyl-L-homocysteine + 7-methoxy-5,3',4'-tetrahydroxyflavone
-
?
S-adenosyl-L-methionine + 5,7,4'-trihydroxyflavanone i.e. naringenin, about 30% of the activity with 5,7,4'-trihydroxyflavone (i.e. apigenin) Hordeum vulgare S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyflavanone i.e. sakuranetin ?
S-adenosyl-L-methionine + 5,7,4'-trihydroxyflavone i.e. apigenin. The product genkwanin exhibits a stronger inhibitory effect on the germination of spores from pathogenic fungi as compared to the effect of apigenin Hordeum vulgare S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyflavone i.e. genkwanin ?
S-adenosyl-L-methionine + 5,7,4'-trihydroxyflavone i.e. apigenin, sequential bi-bi mechanism Hordeum vulgare S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyflavone i.e. genkwanin ?

Subunits

Subunits Comment Organism
? x * 42300, calculated from sequence Hordeum vulgare

Synonyms

Synonyms Comment Organism
F1-OMT
-
Hordeum vulgare
flavonoid 7-O-methyltransferase ambiguous Hordeum vulgare
flavonoid 7-OMT ambiguous Hordeum vulgare

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
assay at Hordeum vulgare

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Hordeum vulgare