Cloned (Comment) | Organism |
---|---|
gene rrp8, sequence comparisons and phylogenetic analysis, quantitative enzyme expression analysis, recombinant expression in Saccharomyces cereviaie Rrp8 deficient mutant strain without complementation by the human Rrp8 enzyme | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | siRNA-mediated depletion of NML in HCT-116 cells, impact of an NML knockdown on m1A1322 methylation in HCT-116 cells | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleolus | NML is primarily localized in the nucleolus | Homo sapiens | 5730 | - |
nucleus | - |
Homo sapiens | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | 25S/28S rRNA of Homo sapiens contains a single m1A methylation in the helix 25.1. Human enzyme NML is able to methylate yeast 25S rRNA in vivo, cf. Ec 2.1.1.287 | ? | - |
- |
|
S-adenosyl-L-methionine + adenine1322 in 28S rRNA | Homo sapiens | - |
S-adenosyl-L-homocysteine + N1-methyladenine1322 in 28S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O43159 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HCT-116 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | 25S/28S rRNA of Homo sapiens contains a single m1A methylation in the helix 25.1. Human enzyme NML is able to methylate yeast 25S rRNA in vivo, cf. Ec 2.1.1.287 | Homo sapiens | ? | - |
- |
|
S-adenosyl-L-methionine + adenine1322 in 28S rRNA | - |
Homo sapiens | S-adenosyl-L-homocysteine + N1-methyladenine1322 in 28S rRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
m1A large subunit rRNA methyltransferase | - |
Homo sapiens |
More | cf. EC 2.1.1.287 | Homo sapiens |
NML | - |
Homo sapiens |
nucleomethylin | - |
Homo sapiens |
ribosomal RNA-processing protein 8 | UniProt | Homo sapiens |
Rrp8 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | the N1-methyladenosine in helix 25.1 of 25/28S rRNA seems to be highly conserved | Homo sapiens |
malfunction | siRNA-mediated depletion of NML is highly efficient at mRNA level, impact of an NML knockdown on m1A1322 methylation in HCT-116 cells. Depletion of NML leads to abnormal and altered nucleolar morphology | Homo sapiens |
physiological function | the human nucleomethylin (NML or RRP8) fails to complement the pre-rRNA processing phenotype and the growth cold sensitivity of Saccharomyces cerevisiae DELTArrp8 mutant strain | Homo sapiens |