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Literature summary for 2.1.1.B114 extracted from
- Hyperthermophilic DNA methyltransferase M.PabI from the archaeon Pyrococcus abyssi (2006), Appl. Environ. Microbiol., 72, 5367-5375.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pyrococcus abyssi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | 50% inhibition observed at 0.3 mM | Pyrococcus abyssi |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000159 | - |
oligoduplex 5'-GGACGCTTCACCGGATGTm6ACAGGCATGCGACGACCCCTAG-3'/3'-CCTGCGAAGTGGCCTACATGTCCGTACGCTGCTGGGGATC-5' | pH 7.5, 75°C | Pyrococcus abyssi | |
| 0.00128 | - |
S-adenosyl-L-methionine | pH 7.5, 75°C | Pyrococcus abyssi | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no inhibition by Mg2+, Ca2+, or Mn2+ | Pyrococcus abyssi | |
| NaCl | activity is optimal at an NaCl concentration of 200 to 500 mM | Pyrococcus abyssi | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
gel filtration | Pyrococcus abyssi |
| 52455 | - |
1 * 52455, calculated from sequence | Pyrococcus abyssi |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + DNA containing the recognition sequence 5'-GTAC-3' | Pyrococcus abyssi | - |
S-adenosyl-L-homocysteine + DNA containing the methylated recognition sequence 5'-GTm6AC-3' | - |
? | |
| S-adenosyl-L-methionine + DNA containing the recognition sequence 5'-GTAC-3' | Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 | - |
S-adenosyl-L-homocysteine + DNA containing the methylated recognition sequence 5'-GTm6AC-3' | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus abyssi | Q9V2B5 | - |
- |
| Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 | Q9V2B5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pyrococcus abyssi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + DNA containing the recognition sequence 5'-GTAC-3' | - |
Pyrococcus abyssi | S-adenosyl-L-homocysteine + DNA containing the methylated recognition sequence 5'-GTm6AC-3' | - |
? | |
| S-adenosyl-L-methionine + DNA containing the recognition sequence 5'-GTAC-3' | - |
Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 | S-adenosyl-L-homocysteine + DNA containing the methylated recognition sequence 5'-GTm6AC-3' | - |
? | |
| S-adenosyl-L-methionine + oligoduplex 5'-GGACGCTTCACCGGATGTm6ACAGGCATGCGACGACCCCTAG-3'/3'-CCTGCGAAGTGGCCTACATGTCCGTACGCTGCTGGGGATC-5' | - |
Pyrococcus abyssi | S-adenosyl-L-homocysteine + oligoduplex 5'-GGACGCTTCACCGGATGTACAGGCATGCGACGACCCCTAG-3'/3'-CCTGCGAAGTGGCCTACATGTCCGTACGCTGCTGGGGATC-5' | - |
? | |
| S-adenosyl-L-methionine + oligoduplex 5'-GGACGCTTCACCGGATGTm6ACAGGCATGCGACGACCCCTAG-3'/3'-CCTGCGAAGTGGCCTACATGTCCGTACGCTGCTGGGGATC-5' | - |
Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 | S-adenosyl-L-homocysteine + oligoduplex 5'-GGACGCTTCACCGGATGTACAGGCATGCGACGACCCCTAG-3'/3'-CCTGCGAAGTGGCCTACATGTCCGTACGCTGCTGGGGATC-5' | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 52455, calculated from sequence | Pyrococcus abyssi |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 5'-GTAC-3'N6-adenine methyltransferase | - |
Pyrococcus abyssi |
| M.PabI | - |
Pyrococcus abyssi |
| M.PabI | PabI constitutes a type II restriction-modification gene complex | Pyrococcus abyssi |
| PAB2246 | locus name | Pyrococcus abyssi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
- |
Pyrococcus abyssi |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | 95 | 65°C: about 40% of maximal activity, 95°C: about 40% of maximal activity | Pyrococcus abyssi |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
9 min, 50% of the activity is retained | Pyrococcus abyssi |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.041 | - |
oligoduplex 5'-GGACGCTTCACCGGATGTm6ACAGGCATGCGACGACCCCTAG-3'/3'-CCTGCGAAGTGGCCTACATGTCCGTACGCTGCTGGGGATC-5' | pH 7.5, 75°C | Pyrococcus abyssi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | 6.7 | at 75°C | Pyrococcus abyssi |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7.5 | pH 3.0: about 55% of maximal activity, pH 7.5: about 50% of maximal activity | Pyrococcus abyssi |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 257 | - |
oligoduplex 5'-GGACGCTTCACCGGATGTm6ACAGGCATGCGACGACCCCTAG-3'/3'-CCTGCGAAGTGGCCTACATGTCCGTACGCTGCTGGGGATC-5' | pH 7.5, 75°C | Pyrococcus abyssi |
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