Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DNA | increased activity on Sul7d, but not Cren7, in the presence of DNA | Sulfolobus islandicus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Sulfolobus islandicus |
Protein Variants | Comment | Organism |
---|---|---|
G38A | methyltransferase activity is completely abolished | Sulfolobus islandicus |
additional information | aKMT4-8A mutant (a mutant with all eight lysine residues mutated to alanine): automethylation is almost completely removed in the presence of substrates, such as Cren7 and Sul7d. Retains significant automethylation activity in the absence of substrates. Mutant with abrogated automethylation shows a more than 150% increase in methylation of substrates | Sulfolobus islandicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00112 | - |
[Sul7d-chromatin-protein] L-lysine | pH 8.0, 50°C, in presence of DNA | Sulfolobus islandicus | |
0.00397 | - |
[Sul7d-chromatin-protein] L-lysine | pH 8.0, 50°C, in absence of DNA | Sulfolobus islandicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + [Sul7d-chromatin-protein] L-lysine | Sulfolobus islandicus | the enzyme methylates Sul7d in the chromatin context | S-adenosyl-L-homocysteine + [Sul7d-chromatin-protein] N6-methyl-L-lysine | - |
? | |
S-adenosyl-L-methionine + [Sul7d-chromatin-protein] L-lysine | Sulfolobus islandicus Rey15A | the enzyme methylates Sul7d in the chromatin context | S-adenosyl-L-homocysteine + [Sul7d-chromatin-protein] N6-methyl-L-lysine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfolobus islandicus | F0NBH8 | - |
- |
Sulfolobus islandicus Rey15A | F0NBH8 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Sulfolobus islandicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme can self-methylate intramolecularly. The substrates Sul7d, RPL11, and Cren7 inhibit automethylation in a concentration-dependent manner. This suggests that the enzyme transfers a methyl group from incoming S-adenosyl-L-methionine to itself in the absence of substrates. When the enzyme associates with the proper substrates, however, the enzyme prefers to transfer the methyl group to the substrates rather than to itself | Sulfolobus islandicus | ? | - |
? | |
additional information | the enzyme can self-methylate intramolecularly. The substrates Sul7d, RPL11, and Cren7 inhibit automethylation in a concentration-dependent manner. This suggests that the enzyme transfers a methyl group from incoming S-adenosyl-L-methionine to itself in the absence of substrates. When the enzyme associates with the proper substrates, however, the enzyme prefers to transfer the methyl group to the substrates rather than to itself | Sulfolobus islandicus Rey15A | ? | - |
? | |
S-adenosyl-L-methionine + [Cren7-chromatin-protein] L-lysine | - |
Sulfolobus islandicus | S-adenosyl-L-homocysteine + [Cren7-chromatin-protein] N6-methyl-L-lysine | - |
? | |
S-adenosyl-L-methionine + [Cren7-chromatin-protein] L-lysine | - |
Sulfolobus islandicus Rey15A | S-adenosyl-L-homocysteine + [Cren7-chromatin-protein] N6-methyl-L-lysine | - |
? | |
S-adenosyl-L-methionine + [Csl4-protein] L-lysine | - |
Sulfolobus islandicus | S-adenosyl-L-homocysteine + [Csl4-protein] N6-methyl-L-lysine | - |
? | |
S-adenosyl-L-methionine + [Csl4-protein] L-lysine | - |
Sulfolobus islandicus Rey15A | S-adenosyl-L-homocysteine + [Csl4-protein] N6-methyl-L-lysine | - |
? | |
S-adenosyl-L-methionine + [Rrp4-protein] L-lysine | - |
Sulfolobus islandicus | S-adenosyl-L-homocysteine + [Rrp4-protein] N6-methyl-L-lysine | - |
? | |
S-adenosyl-L-methionine + [Rrp42-protein] L-lysine | - |
Sulfolobus islandicus | S-adenosyl-L-homocysteine + [Rrp42-protein] N6-methyl-L-lysine | - |
? | |
S-adenosyl-L-methionine + [Sul7d-chromatin-protein] L-lysine | - |
Sulfolobus islandicus | S-adenosyl-L-homocysteine + [Sul7d-chromatin-protein] N6-methyl-L-lysine | - |
? | |
S-adenosyl-L-methionine + [Sul7d-chromatin-protein] L-lysine | the enzyme methylates Sul7d in the chromatin context | Sulfolobus islandicus | S-adenosyl-L-homocysteine + [Sul7d-chromatin-protein] N6-methyl-L-lysine | - |
? | |
S-adenosyl-L-methionine + [Sul7d-chromatin-protein] L-lysine | - |
Sulfolobus islandicus Rey15A | S-adenosyl-L-homocysteine + [Sul7d-chromatin-protein] N6-methyl-L-lysine | - |
? | |
S-adenosyl-L-methionine + [Sul7d-chromatin-protein] L-lysine | the enzyme methylates Sul7d in the chromatin context | Sulfolobus islandicus Rey15A | S-adenosyl-L-homocysteine + [Sul7d-chromatin-protein] N6-methyl-L-lysine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aKMT4 | - |
Sulfolobus islandicus |
SiRe_1449 | - |
Sulfolobus islandicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | - |
Sulfolobus islandicus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 80 | 30°C: about 40% of maximal activity, 80°C: about 40% of maximal activity | Sulfolobus islandicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Sulfolobus islandicus |