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Literature summary for 2.1.1.B109 extracted from

  • Noma, A.; Yi, S.; Katoh, T.; Takai, Y.; Suzuki, T.; Suzuki, T.
    Actin-binding protein ABP140 is a methyltransferase for 3-methylcytidine at position 32 of tRNAs in Saccharomyces cerevisiae (2011), RNA, 17, 1111-1119.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.B109

Cloned(Commentary)

Cloned (Comment) Organism
gene ABP140 or TRM140, DNA and amino acid sequence determination and analysis, expression of His6-tagged wild-type in Saccharomyces cerevisiae, expression of deletion mutant delD602-Q621 in the abp140DELTA null mutant strain Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
D466A site-directed mutagenesis, inactive mutant Saccharomyces cerevisiae
D547A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type Saccharomyces cerevisiae
F481A site-directed mutagenesis, the mutant shows unaltered activity compared to the wild-type Saccharomyces cerevisiae
K559A site-directed mutagenesis, the mutant shows unaltered activity compared to the wild-type Saccharomyces cerevisiae
additional information deletion of the conserved region near the C-terminus, i.e. delD602-Q621 and construction of the abp140DELTA knockout strain, expression of delD602-Q621 in the null mutant strain. Complementation of the yor239wD strain with plasmid-encoded ABP140 containing various mutations, in vitro reconstitution of m3C32 by recombinant Trm140p Saccharomyces cerevisiae
additional information short-hairpin(sh)RNA knockdown of METTL2B mRNA in HeLa cells Homo sapiens
Y569A site-directed mutagenesis, the mutant shows unaltered activity compared to the wild-type Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
actin filament subcellular localization of Abp140p to actin filaments Saccharomyces cerevisiae 5884
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71500
-
 2 * 71500, about, sequence calculation Saccharomyces cerevisiae
150000
-
 about, recombinant His6-tagged wild-type Abp140p, native PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + cytosine32 in tRNASer/Thr Saccharomyces cerevisiae
-
 S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNASer/Thr
-
 ?
S-adenosyl-L-methionine + cytosine32 in tRNASer/Thr Homo sapiens
-
 S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNASer/Thr
-
 ?
S-adenosyl-L-methionine + cytosine32 in tRNASer/Thr Saccharomyces cerevisiae BY4742
-
 S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNASer/Thr
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q6P1Q9 gene TRM140
-
Saccharomyces cerevisiae
-
 gene ABP140 or TRM140
-
Saccharomyces cerevisiae BY4742
-
 gene ABP140 or TRM140
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Abp140p from Saccharoymces cerevisiae by nickel affinity chromatography and dialysis Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + cytosine32 in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytosine32 in tRNA the actin-binding protein ABP140 is responsible for m3C formation in both tRNAThr1 Homo sapiens
a
S-adenosyl-L-methionine + cytosine32 in tRNA = S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNA the actin-binding protein ABP140 is responsible for m3C formation in both tRNAThr1, and is also active with tRNASer1. In Saccharomyces cerevisiae, 3-methylcytidine is found at position 32 of the tRNAs for Thr and Ser Saccharomyces cerevisiae
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information secondary structures of Saccharomyces cerevisiae tRNAThr1 and tRNASer1 with modified nucleosides, including 3-methylcytidine, overview Saccharomyces cerevisiae ?
-
 ?
additional information secondary structures of Saccharomyces cerevisiae tRNAThr1 and tRNASer1 with modified nucleosides, including 3-methylcytidine, overview Saccharomyces cerevisiae BY4742 ?
-
 ?
S-adenosyl-L-methionine + cytosine32 in tRNASer/Thr
-
 Saccharomyces cerevisiae S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNASer/Thr
-
 ?
S-adenosyl-L-methionine + cytosine32 in tRNASer/Thr
-
 Homo sapiens S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNASer/Thr
-
 ?
S-adenosyl-L-methionine + cytosine32 in tRNASer/Thr tRNASer1 is not methylated by Abp140p under the same condition as the methylation of tRNAThr1 in vitro, overview. Some essential factor(s) is required for m3C formation in tRNASer1 Saccharomyces cerevisiae S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNASer/Thr mass spectrometric analysis of tRNA products ?
S-adenosyl-L-methionine + cytosine32 in tRNASer/Thr
-
 Saccharomyces cerevisiae BY4742 S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNASer/Thr
-
 ?
S-adenosyl-L-methionine + cytosine32 in tRNASer/Thr tRNASer1 is not methylated by Abp140p under the same condition as the methylation of tRNAThr1 in vitro, overview. Some essential factor(s) is required for m3C formation in tRNASer1 Saccharomyces cerevisiae BY4742 S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNASer/Thr mass spectrometric analysis of tRNA products ?

Subunits

Subunits Comment Organism
homodimer 2 * 71500, about, sequence calculation Saccharomyces cerevisiae
More ABP140 consists of an N-terminal actin-binding motif, a +1 frameshift site, and the C-terminal Ado-Met binding motif. The homodimer is formed through covalent linkage(s) other than disulfide bond Saccharomyces cerevisiae
More ABP140 consists of an N-terminal actin-binding sequence and a C-terminal S-adenosylmethionine binding motif Homo sapiens

Synonyms

Synonyms Comment Organism
Abp140p
-
 Saccharomyces cerevisiae
Abp140p
-
 Homo sapiens
methyltransferase-like protein 2B
-
 Homo sapiens
METTL2B
-
 Homo sapiens
Trm140p
-
 Saccharomyces cerevisiae
Trm140p
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Homo sapiens
S-adenosyl-L-methionine the conserved S-adenosyl-L-methionine binding motif in ABP140 is required for m3C formation Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction deletion of the actin-binding sequence in ABP140 does not affect m3C formation. The yor240wDELTA strain, lacking the half of gene ABP140, hatbors tRNAs Ser and Thr that are not m3C methylated, mass spectrometric aanalysis Saccharomyces cerevisiae
malfunction specific reduction of m3C formation in HeLa cells by siRNA-mediated knockdown of the human orthologue of TRM14 Homo sapiens
additional information some partner proteins of METTL2B might be required to reconstitute m3C formation in tRNAs Homo sapiens
additional information subcellular localization of ABP140 to actin filaments is not involved in tRNA modification Saccharomyces cerevisiae
physiological function methyl modification of m3C32 might modulate accurate codon recognition at the A-site and the proper ribosomal positioning at the P-site. Abp140p interacts with filamentous actin and has an activity to cross-link F-actin into a bundle in vitro. In the cell, Abp140p colocalizes with cortical actin patches and cytoplasmic actin filaments, but it is not required for cell growth or organization of F-actin Saccharomyces cerevisiae