Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 (DE3) | Pyrococcus horikoshii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 30000, SDS-PAGE | Pyrococcus horikoshii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] | Pyrococcus horikoshii | the enzyme acts on His600 of elongation factor 2. It is involved in diphthamide biosynthesis | 3 S-adenosyl-L-homocysteine + diphthine-[translation elongation factor 2] | - |
? | |
3 S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] | Pyrococcus horikoshii DSM 12428 | the enzyme acts on His600 of elongation factor 2. It is involved in diphthamide biosynthesis | 3 S-adenosyl-L-homocysteine + diphthine-[translation elongation factor 2] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | O58456 | - |
- |
Pyrococcus horikoshii DSM 12428 | O58456 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] | the enzyme acts on His600 of elongation factor 2. It is involved in diphthamide biosynthesis | Pyrococcus horikoshii | 3 S-adenosyl-L-homocysteine + diphthine-[translation elongation factor 2] | - |
? | |
3 S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] | the enzyme catalyzes the trimethylation in a highly processive manner. It acts on His600 of elongation factor 2. Mono- and dimethylated PhEF2 are detected, while the trimethylated product cannot be detected due to the facile elimination of the trimethylamino group | Pyrococcus horikoshii | 3 S-adenosyl-L-homocysteine + diphthine-[translation elongation factor 2] | - |
? | |
3 S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] | the enzyme acts on His600 of elongation factor 2. It is involved in diphthamide biosynthesis | Pyrococcus horikoshii DSM 12428 | 3 S-adenosyl-L-homocysteine + diphthine-[translation elongation factor 2] | - |
? | |
3 S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] | the enzyme catalyzes the trimethylation in a highly processive manner. It acts on His600 of elongation factor 2. Mono- and dimethylated PhEF2 are detected, while the trimethylated product cannot be detected due to the facile elimination of the trimethylamino group | Pyrococcus horikoshii DSM 12428 | 3 S-adenosyl-L-homocysteine + diphthine-[translation elongation factor 2] | - |
? | |
S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-(dimethylamino)propyl]-L-histidine-[translation elongation factor 2] | the enzyme catalyzes the trimethylation in a highly processive manner. It acts on His600 of elongation factor 2. Mono- and dimethylated PhEF2 are detected, while the trimethylated product cannot be detected due to the facile elimination of the trimethylamino group | Pyrococcus horikoshii | S-adenosyl-L-homocysteine + diphthine-[translation elongation factor 2] | - |
? | |
S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-(dimethylamino)propyl]-L-histidine-[translation elongation factor 2] | the enzyme catalyzes the trimethylation in a highly processive manner. It acts on His600 of elongation factor 2. Mono- and dimethylated PhEF2 are detected, while the trimethylated product cannot be detected due to the facile elimination of the trimethylamino group | Pyrococcus horikoshii DSM 12428 | S-adenosyl-L-homocysteine + diphthine-[translation elongation factor 2] | - |
? | |
S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-(methylamino)propyl]-L-histidine-[translation elongation factor 2] | the enzyme catalyzes the trimethylation in a highly processive manner. It acts on His600 of elongation factor 2. Mono- and dimethylated PhEF2 are detected, while the trimethylated product cannot be detected due to the facile elimination of the trimethylamino group | Pyrococcus horikoshii | S-adenosyl-L-homocysteine + 2-[(3S)-3-carboxy-3-(dimethylamino)propyl]-L-histidine-[translation elongation factor 2] | - |
? | |
S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-(methylamino)propyl]-L-histidine-[translation elongation factor 2] | the enzyme catalyzes the trimethylation in a highly processive manner. It acts on His600 of elongation factor 2. Mono- and dimethylated PhEF2 are detected, while the trimethylated product cannot be detected due to the facile elimination of the trimethylamino group | Pyrococcus horikoshii DSM 12428 | S-adenosyl-L-homocysteine + 2-[(3S)-3-carboxy-3-(dimethylamino)propyl]-L-histidine-[translation elongation factor 2] | - |
? | |
S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] | the enzyme catalyzes the trimethylation in a highly processive manner. It acts on His600 of elongation factor 2. Mono- and dimethylated PhEF2 are detected, while the trimethylated product cannot be detected due to the facile elimination of the trimethylamino group | Pyrococcus horikoshii | S-adenosyl-L-homocysteine + 2-[(3S)-3-carboxy-3-(methylamino)propyl]-L-histidine-[translation elongation factor 2] | - |
? | |
S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] | the enzyme catalyzes the trimethylation in a highly processive manner. It acts on His600 of elongation factor 2. Mono- and dimethylated PhEF2 are detected, while the trimethylated product cannot be detected due to the facile elimination of the trimethylamino group | Pyrococcus horikoshii DSM 12428 | S-adenosyl-L-homocysteine + 2-[(3S)-3-carboxy-3-(methylamino)propyl]-L-histidine-[translation elongation factor 2] | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, SDS-PAGE | Pyrococcus horikoshii |
Synonyms | Comment | Organism |
---|---|---|
Dph5 | - |
Pyrococcus horikoshii |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in diphthamide biosynthesis | Pyrococcus horikoshii |