Cloned (Comment) | Organism |
---|---|
gene for the 88000 Mr PHS subunit cloned | Streptomyces antibioticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
HEPES buffer | - |
Streptomyces antibioticus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | intracellular membrane associated | Streptomyces antibioticus | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
1 * 36000, SDS-PAGE | Streptomyces antibioticus |
37000 | - |
gel filtration, chromatography in 0.5 M NaCl | Streptomyces antibioticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 3-hydroxyanthranilate | Streptomyces sp. | - |
S-adenosyl-L-homocysteine + 3-hydroxy-4-methylanthranilate | - |
? | |
S-adenosyl-L-methionine + 3-hydroxyanthranilate | Streptomyces anulatus | - |
S-adenosyl-L-homocysteine + 3-hydroxy-4-methylanthranilate | - |
? | |
S-adenosyl-L-methionine + 3-hydroxyanthranilate | Streptomyces antibioticus | involved in the biosynthesis of the antibiotic actinomycin | S-adenosyl-L-homocysteine + 3-hydroxy-4-methylanthranilate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces antibioticus | - |
- |
- |
Streptomyces anulatus | - |
- |
- |
Streptomyces sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces antibioticus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Streptomyces antibioticus | - |
Storage Stability | Organism |
---|---|
-70°C, 5% glycerol, 50 mM sodium phosphate, pH 7.0, 10 mM Na2EDTA, 5 mM 2-mercaptoethanol, 1 mM phenylmethylsulfonyl fluoride, quite stable, can be frozen and thawed several times without appreciable loss of activity | Streptomyces antibioticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | does not catalyze the methylation of 3-hydroxy-DL-kynurenine | Streptomyces antibioticus | ? | - |
? | |
S-adenosyl-L-methionine + 3-hydroxyanthranilate | - |
Streptomyces sp. | S-adenosyl-L-homocysteine + 3-hydroxy-4-methylanthranilate | - |
? | |
S-adenosyl-L-methionine + 3-hydroxyanthranilate | - |
Streptomyces anulatus | S-adenosyl-L-homocysteine + 3-hydroxy-4-methylanthranilate | - |
? | |
S-adenosyl-L-methionine + 3-hydroxyanthranilate | involved in the biosynthesis of the antibiotic actinomycin | Streptomyces antibioticus | S-adenosyl-L-homocysteine + 3-hydroxy-4-methylanthranilate | - |
? | |
S-adenosyl-L-methionine + 3-hydroxyanthranilic acid | - |
Streptomyces sp. | S-adenosyl-L-homocysteine + 4-methyl-3-hydroxyanthranilic acid | - |
? | |
S-adenosyl-L-methionine + 3-hydroxyanthranilic acid | - |
Streptomyces antibioticus | S-adenosyl-L-homocysteine + 4-methyl-3-hydroxyanthranilic acid | - |
? | |
S-adenosyl-L-methionine + 3-hydroxyanthranilic acid | - |
Streptomyces anulatus | S-adenosyl-L-homocysteine + 4-methyl-3-hydroxyanthranilic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 36000, SDS-PAGE | Streptomyces antibioticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | sodium phosphate buffer | Streptomyces antibioticus |
7 | 8 | Tris buffer | Streptomyces antibioticus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Streptomyces antibioticus |