Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | requires thiol-protecting agents for activity | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag2+ | 100% inhibition at 1 mM | Pseudomonas sp. | |
Hg2+ | 100% inhibition at 0.05 mM | Pseudomonas sp. | |
iodoacetamide | 55% inhibition at 0.5 mM | Pseudomonas sp. | |
additional information | EDTA and Mg2+ are not inhibitory | Pseudomonas sp. | |
N-ethylmaleimide | 56% inhibition at 0.05 mM | Pseudomonas sp. | |
Phenylmercuriacetate | 95% inhibition at 0.025 mM | Pseudomonas sp. | |
S-adenosyl-L-homocysteine | competitive inhibition | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
2-Methylbutyraldoxime | - |
Pseudomonas sp. | |
0.15 | - |
S-adenosyl-L-methionine | - |
Pseudomonas sp. | |
0.16 | - |
3-Methylbutyraldoxime | - |
Pseudomonas sp. | |
0.24 | 0.27 | Isobutyraldoxime | - |
Pseudomonas sp. | |
0.67 | - |
n-Pentanaldoxime | - |
Pseudomonas sp. | |
0.98 | - |
n-Butyraldoxime | - |
Pseudomonas sp. | |
1.11 | - |
Methacrylaldoxime | - |
Pseudomonas sp. | |
1.54 | - |
n-Propionaldoxime | - |
Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Mg2+ has no significant effect | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37500 | - |
2 * 37500, SDS-PAGE | Pseudomonas sp. |
84000 | - |
gel filtration | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
N.C.I.B. 11652 | - |
Pseudomonas sp. NCIB 11652 | - |
N.C.I.B. 11652 | - |
Oxidation Stability | Organism |
---|---|
labile to O2, thiol-protecting agents are necessary | Pseudomonas sp. |
Purification (Comment) | Organism |
---|---|
220fold | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
247.4 | - |
- |
Pseudomonas sp. |
Storage Stability | Organism |
---|---|
-15°C, 100 mM potassium phosphate buffer, pH 7.5, 5 mM DTT, several months | Pseudomonas sp. |
0°C, (NH4)2SO4-paste has a half life of 10 weeks | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methylbutyraldoxime + S-adenosyl-L-methionine | highest activity | Pseudomonas sp. | 2-methylbutyraldoxime methyl ether + S-adenosyl-L-homocysteine | - |
? | |
2-methylbutyraldoxime + S-adenosyl-L-methionine | highest activity | Pseudomonas sp. NCIB 11652 | 2-methylbutyraldoxime methyl ether + S-adenosyl-L-homocysteine | - |
? | |
3-methylbutyraldoxime + S-adenosyl-L-methionine | - |
Pseudomonas sp. | 3-methylbutyraldoxime methyl ether + S-adenosyl-L-homocysteine | - |
? | |
3-methylbutyraldoxime + S-adenosyl-L-methionine | - |
Pseudomonas sp. NCIB 11652 | 3-methylbutyraldoxime methyl ether + S-adenosyl-L-homocysteine | - |
? | |
isobutyraldoxime + S-adenosyl-L-methionine | - |
Pseudomonas sp. | isobutyraldoxime methyl ether + S-adenosyl-L-homocysteine | - |
? | |
isobutyraldoxime + S-adenosyl-L-methionine | - |
Pseudomonas sp. NCIB 11652 | isobutyraldoxime methyl ether + S-adenosyl-L-homocysteine | - |
? | |
methacrylaldoxime + S-adenosyl-L-methionine | - |
Pseudomonas sp. | methacrylaldoxime methyl ether + S-adenosyl-L-homocysteine | - |
? | |
additional information | no reaction with n-hexanaldoxime, benzaldoxime, 2-butoxime | Pseudomonas sp. | ? | - |
? | |
additional information | no reaction with n-hexanaldoxime, benzaldoxime, 2-butoxime | Pseudomonas sp. NCIB 11652 | ? | - |
? | |
n-butyraldoxime + S-adenosyl-L-methionine | - |
Pseudomonas sp. | n-butyraldoxime methyl ether + S-adenosyl-L-homocysteine | - |
? | |
n-pentanaldoxime + S-adenosyl-L-methionine | - |
Pseudomonas sp. | n-pentanaldoxime methyl ether + S-adenosyl-L-homocysteine | - |
? | |
n-propionaldoxime + S-adenosyl-L-methionine | - |
Pseudomonas sp. | n-propionaldoxime methyl ether + S-adenosyl-L-homocysteine | - |
? | |
n-propionaldoxime + S-adenosyl-L-methionine | - |
Pseudomonas sp. NCIB 11652 | n-propionaldoxime methyl ether + S-adenosyl-L-homocysteine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 37500, SDS-PAGE | Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
temperature sensitive: 0-3°C are necessary during purification | Pseudomonas sp. |
35 | - |
rapid inactivation above | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10.25 | - |
- |
Pseudomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
9 | 10.4 | less than 50% of maximal activity below pH 9.0, almost no activity above pH 10.4 | Pseudomonas sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
S-adenosyl-L-homocysteine | - |
Pseudomonas sp. |