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Literature summary for 2.1.1.90 extracted from

  • Dong, M.; Gonzalez, T.; Klems, M.; Steinberg, L.; Chen, W.; Papoutsakis, E.; Bahnson, B.
    In vitro methanol production from methyl coenzyme M using the Methanosarcina barkeri MtaABC protein complex (2017), Biotechnol. Prog., 33, 1243-1249 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methanol + cob(I)alamin Methanosarcina barkeri
-
methyl-cob(III)alamin + H2O
-
r
methanol + cob(I)alamin Methanosarcina barkeri DSM 804
-
methyl-cob(III)alamin + H2O
-
r

Organism

Organism UniProt Comment Textmining
Methanosarcina barkeri Q48949 and Q46EH3 and Q46EH4 subunits MtaA and MtaB and MtaC
-
Methanosarcina barkeri DSM 804 Q48949 and Q46EH3 and Q46EH4 subunits MtaA and MtaB and MtaC
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Q Sepharose column chromatography Methanosarcina barkeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methanol + cob(I)alamin
-
Methanosarcina barkeri methyl-cob(III)alamin + H2O
-
r
methanol + cob(I)alamin
-
Methanosarcina barkeri DSM 804 methyl-cob(III)alamin + H2O
-
r

Subunits

Subunits Comment Organism
? x * 49000 + x * 36000 + x * 24000, SDS-PAGE Methanosarcina barkeri

Synonyms

Synonyms Comment Organism
methanol:coenzyme M methyltransferase
-
Methanosarcina barkeri
methanol:CoM methyltransferase complex
-
Methanosarcina barkeri
MtaABC
-
Methanosarcina barkeri